QX77

Catalog No.S6797 Batch:S679701

Technical Data

Formula	C₁₆H₁₃ClN₂O₂
Molecular Weight	300.74	CAS No.	1798331-92-6
Solubility (25°C)*	In vitro	DMSO	15 mg/mL (49.87 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

QX77 is a novel activator of chaperone-mediated autophagy (CMA). QX77 induces the up-regulation of Rab11 expression and up-regulates LAMP2A expression.

Targets

CMA ^[1]

In vitro

QX77, an activator of CMA, eliminates the effects of milk fat globule-EGF factor 8 (MFG-E8) on TGF-β1-induced human pancreatic stellate cells (PSCs) activation. QX77 mitigates the suppressive effect of MFG-E8 on oxidative stress in activated PSCs.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Human pancreatic stellate cells Concentrations 5 ng/ml Incubation Time 24 h Method Human pancreatic stellate cells (1×10⁶/well) are treated with 5 ng/ml TGF-β1 with or without 20 ng/ml MFG-E8 for 24 h. 5 ng/mL QX77 is added simultaneously with 20 ng/mL MFG-E8 in TGF-β1-treated HPSCs. 24 h later, the cells are collected for various measurements. Western blot analyse the expression of GRP78, p-PERK, PERK, LAMP2A and MEF2D in the HPSCs .

Cell Assay:^{^[2]}

Cell lines
Human pancreatic stellate cells
Concentrations
5 ng/ml
Incubation Time
24 h
Method
Human pancreatic stellate cells (1×10⁶/well) are treated with 5 ng/ml TGF-β1 with or without 20 ng/ml MFG-E8 for 24 h. 5 ng/mL QX77 is added simultaneously with 20 ng/mL MFG-E8 in TGF-β1-treated HPSCs. 24 h later, the cells are collected for various measurements. Western blot analyse the expression of GRP78, p-PERK, PERK, LAMP2A and MEF2D in the HPSCs .

References

Selleck's QX77 has been cited by 2 publications

Impaired degradation of PLCG1 by chaperone-mediated autophagy promotes cellular senescence and intervertebral disc degeneration [ Autophagy, 2024, 10.1080/15548627.2024.2395797]	PubMed: 39212196
Milk Fat Globule-EGF Factor 8 Alleviates Pancreatic Fibrosis by Inhibiting ER Stress-Induced Chaperone-Mediated Autophagy in Mice [ Front Pharmacol, 2021, 12:707259]	PubMed: 34421598

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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