Home Autophagy Autophagy activator SMER28 For research use only.

SMER28

SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.

SMER28 Chemical Structure

SMER28 Chemical Structure

CAS No. 307538-42-7

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SMER28 Related Products

Signaling Pathway

Autophagy Signaling Pathways

Autophagy Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC12 Function assay 43 uM Enhancement of autophagy in rat PC12 cells assessed as decrease in alpha-synuclein A53T mutant level at 43 uM by immunoblotting 17486044
COS7 Function assay 47 uM 48 hrs Inhibition of EGFP-tagged huntingtin aggregation expressed in african green monkey COS7 cells at 47 uM after 48 hrs by Western blot analysis 17486044
COS7 Function assay 47 uM 48 hrs Inhibition of EGFP-tagged huntingtin expressed in african green monkey COS7 cells assessed as protection against protein-induced cell death at 47 uM after 48 hrs by Western blot analysis 17486044
HeLa Function assay 47 uM 24 hrs Induction of autophagy in human HeLa cells harboring MAP1LC3 gene at 47 uM after 24 hrs by DAPI staining-based fluorescence microscopy 17486044
HeLa Function assay 47 uM 24 hrs Increase in MAP1LC3 level in bafilomycin A1-treated human HeLa cells at 47 uM treated fro 24 hrs before bafilomycin A1 challenge by immunoblotting analysis 17486044
PC12 Function assay 47 uM 8 hrs Enhancement of rapamycin-induced clearance of alpha-synuclein A53T mutant protein in rat PC12 cells at 47 uM after 8 hrs by immunoblotting 17486044
COS7 Function assay 47 uM 24 hrs Inhibition of EGFP-tagged huntingtin aggregation expressed in african green monkey COS7 cells at 47 uM after 24 hrs by Western blot analysis in presence of 0.2 uM rapamycin 17486044
PC12 Function assay 5 mg/ml Enhancement of autophagy in rat PC12 cells assessed as decrease in alpha-synuclein A53T mutant level at 5 mg/ml by immunoblotting 17486044
PC12 Function assay 5 mg/ml Enhancement of autophagy in rat PC12 cells assessed as decrease in alpha-synuclein A53T mutant level at 5 mg/ml by densitometry 17486044
PC12 Function assay 47 uM Enhancement of autophagy in rat PC12 cells assessed as decrease in alpha-synuclein A53T mutant level at 47 uM by immunoblotting 17486044
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Biological Activity

Description SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.
Targets
Autophagy [1]
In vitro
In vitro SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment[1].
Cell Research Cell lines MEF cells overexpressing βCTF/ N2a-APP cells/ Rat primary neuronal cultures
Concentrations 10μM in MEF cells/0-50μM in N2a-APP cells and rat primary neuronal cultures
Incubation Time 16hrs
Method MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10 μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.

Chemical Information & Solubility

Molecular Weight 264.12 Formula

C11H10BrN3
CAS No. 307538-42-7 SDF Download SMER28 SDF
Smiles C=CCNC1=NC=NC2=C1C=C(C=C2)Br
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 53 mg/mL ( (200.66 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 53 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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