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MPP+ iodide

Synonyms: N-Methyl-4-Phenylpyridinium Iodide

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide), the metabolite of a neurotoxin MPTP, causes symptom of Parkinson's disease (PD) in animal models by selectively destroying dopaminergic neurons in substantia nigra. MPP+ induces autophagic cell death in SH-SY5Y cells. MPP+ induces dopamine transporter (DAT) externalization in dopaminergic (DA) neurons, but internalization of serotonin transporter (SERT) in serotonergic (5-HT) neurons.

MPP+ iodide Chemical Structure

MPP+ iodide Chemical Structure

CAS No. 36913-39-0

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Description MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide), the metabolite of a neurotoxin MPTP, causes symptom of Parkinson's disease (PD) in animal models by selectively destroying dopaminergic neurons in substantia nigra. MPP+ induces autophagic cell death in SH-SY5Y cells. MPP+ induces dopamine transporter (DAT) externalization in dopaminergic (DA) neurons, but internalization of serotonin transporter (SERT) in serotonergic (5-HT) neurons.
Targets
DAT [1] SERT [1]
In vitro
In vitro

Vacuolin-1 potently and reversibly inhibits the fusion between autophagosomes and lysosomes in mammalian cells, thereby inducing the accumulation of autophagosomes. Vacuolin-1 activates RAB5A to block autophagosome-lysosome fusion. [1]
Cell Research Cell lines HeLa cells
Concentrations 1 μM
Incubation Time 10 min, 2 h
Method

The active RAB5A in cells treated with vacuoin-1 or transfected with RAB5A-CA or RAB5A-DN are examined by a GST-tagged RABEP1 affinity isolation assay.
In Vivo
In vivo

MPP+ iodide decreases mitochondrial oxidation-reduction activity and membrane potential in rat striatum.
Animal Research Animal Models Male Wistar rats
Dosages 40 nM
Administration intrastriatal infusion

Chemical Information & Solubility

Molecular Weight 297.13 Formula

C12H12IN
CAS No. 36913-39-0 SDF --
Smiles [I-].C[N+]1=CC=C(C=C1)C2=CC=CC=C2
Storage (From the date of receipt) 3 years-20°C(in the dark) powder

In vitro
Batch:

DMSO : 72 mg/mL ( (242.31 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 72 mg/mL

Water : 35 mg/mL


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