Chloroquine

Catalog No.S6999 Batch:S699901

Technical Data

Formula	C₁₈H₂₆ClN₃
Molecular Weight	319.87	CAS No.	54-05-7
Solubility (25°C)*	In vitro	DMSO	64 mg/mL (200.08 mM)
		Ethanol	64 mg/mL (200.08 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Chloroquine is an antimalarial drug and autophagy/lysosome inhibitor. Chloroquine also suppresses Toll-like receptor-9 (TLR9) protein expression. Chloroquine is highly effective agianst SARS-CoV-2 (COVID-19) infection with EC50 of 1.13 μM in Vero E6 cells. Chloroquine has anti-HIV-1 activity.

Targets

Autophagy ^[1]	lysosome ^[1]	TLR9 ^[2]	HIV-1 ^[4]	SARS-CoV-2 ^[3] (Vero E6 cells based assay)
				1.13 μM(EC50)

In vitro

Chloroquine is a widely used autophagy inhibitor.^[5]

In vivo

The inhibition of autophagy by chloroquine potentiates the therapeutic effect of PI3K/AKT inhibitors in vitro and in vivo TNBC models, synergizing with taxane-based chemotherapy.^[6]

Protocol (from reference)

Cell Assay:^{^[5]}	Cell lines HL‐60 cells Concentrations 10 µM Incubation Time 48 h Method One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection. (Data sourced from selleck products)
Animal Study:^{^[6]}	Animal Models Female NOD/SCID athymic mice injected with MDAMB231 cells Dosages 30 mg/kg Administration o.g. (Data sourced from selleck products)

Cell Assay:^{^[5]}

Cell lines
HL‐60 cells
Concentrations
10 µM
Incubation Time
48 h
Method
One day before treatment, AML cells were seeded with a density of 2 × 10⁵ cells/mL. Fresh medium containing different concentrations of Ara‐C was added into the culture medium. Cells were further cultured for 24 or 48 h based on experimental purposes. For inhibition of autophagy, HL‐60 cells were treated with 200 nM bafilomycin A1 or 10 µM chloroquine. Overexpression and knockdown were performed by transfection.

(Data sourced from selleck products)

Animal Study:^{^[6]}

Animal Models
Female NOD/SCID athymic mice injected with MDAMB231 cells
Dosages
30 mg/kg
Administration
o.g.

(Data sourced from selleck products)

References

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Selleck's Chloroquine has been cited by 211 publications

VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment [ Signal Transduct Target Ther, 2025, 10(1):26]	PubMed: 39848960
MAPK13 phosphorylates PHGDH and promotes its degradation via chaperone-mediated autophagy during liver injury [ Cell Discov, 2025, 11(1):15]	PubMed: 39962071
S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism [ Nat Commun, 2025, 16(1):509]	PubMed: 39779666
OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model [ Adv Sci (Weinh), 2025, 12(7):e2406700]	PubMed: 39721018
Inhibition of Endoplasmic Reticulum Stress Cooperates with SLC7A11 to Promote Disulfidptosis and Suppress Tumor Growth upon Glucose Limitation [ Adv Sci (Weinh), 2025, 12(7):e2408789]	PubMed: 39739602
Disrupting AGR2/IGF1 paracrine and reciprocal signaling for pancreatic cancer therapy [ Cell Rep Med, 2025, 6(2):101927]	PubMed: 39914384
Inhibiting autophagy selectively prunes dysfunctional tumor vessels and optimizes the tumor immune microenvironment [ Theranostics, 2025, 15(1):258-276]	PubMed: 39744218
DLGAP5 enhances bladder cancer chemoresistance by regulating glycolysis through MYC stabilization [ Theranostics, 2025, 15(6):2375-2392]	PubMed: 39990228
Hypoxia-induced degradation of FTO promotes apoptosis by unmasking RACK1-mediated activation of MTK1-JNK1/2 pathway [ J Adv Res, 2025, S2090-1232(25)00038-4]	PubMed: 39805423
CHMP4C promotes pancreatic cancer progression by inhibiting necroptosis via the RIPK1/RIPK3/MLKL pathway [ J Adv Res, 2025, S2090-1232(25)00058-X]	PubMed: 39870301

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SHIPPING AND STORAGE
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