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Biocytin

Synonyms: (+)-Biocytin, Biotinyl-L-lysine

Biocytin ((+)-Biocytin, Biotinyl-L-lysine) is a conjugate of D-biotin and L-lysine. Biocytin is an attractive neuronal tracer. It labels neurons fast over long distances, and it can be used in conjunction with calcium dyes to report on neuronal activity in retrogradely labeled live neurons.

Biocytin Chemical Structure

Biocytin Chemical Structure

CAS No. 576-19-2

Purity & Quality Control

Batch: S988601 DMSO]75 mg/mL]false]Water]75 mg/mL]false]Ethanol]2 mg/mL]false Purity: 99.43%
99.43

Biocytin Related Products

Biological Activity

Description Biocytin ((+)-Biocytin, Biotinyl-L-lysine) is a conjugate of D-biotin and L-lysine. Biocytin is an attractive neuronal tracer. It labels neurons fast over long distances, and it can be used in conjunction with calcium dyes to report on neuronal activity in retrogradely labeled live neurons.
In vitro
In vitro

Biocytin labels type II cells via ion channels activated by [Ca2+]out reduction, probably "CALHM-like" channels, on the basolateral membrane.[3]
Cell Research Cell lines Taste receptor cells (type II cells)
Concentrations 2 mg/ml
Incubation Time 5 min
Method

Peeled epithelia are mounted on a recording platform and then are examined under a microscope equipped with a 60× water-immersion objective. Recording electrodes are attached to the basolateral membranes of single TBCs. In these voltage-clamp experiments, voltage-gated currents are amplified and filtered at 10 kHz using a voltage-clamp amplifier. Data are digitized using an A/D converter and are then stored using pCLAMP data acquisition and analysis software on a personal computer. The recording electrodes are filled with an intracellular CsCl solution using a pipette with a resistance of approximately 5 MΩ in physiological saline. To immunohistochemically identify cell types, biocytin (2 mg/ml) is injected through the recording electrodes.
In Vivo
In vivo

Biocytin confers stability to the molecules and increases their applicability as neuronal tract tracer.[2]
Animal Research Animal Models Albino rats (Sprague−Dawley)
Dosages --
Administration ICV

Chemical Information & Solubility

Molecular Weight 372.48 Formula
C16H28N4O4S
CAS No. 576-19-2 SDF --
Smiles NC(CCCCNC(=O)CCCCC1SCC2NC(=O)NC12)C(O)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 75 mg/mL ( (201.35 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 75 mg/mL

Ethanol : 2 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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