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DAPI Dihydrochloride

Synonyms: DAPI 2HCl

DAPI Dihydrochloride (DAPI 2HCl) is a fluorescent dye that binds strongly to adenine–thymine-rich regions in DNA.

DAPI Dihydrochloride Chemical Structure

DAPI Dihydrochloride Chemical Structure

CAS No. 28718-90-3

Purity & Quality Control

Batch: S998001 DMSO]70 mg/mL]false]Water]10 mg/mL]false]Ethanol]Insoluble]false Purity: 99.9%
99.9

DAPI Dihydrochloride Related Products

Biological Activity

Description DAPI Dihydrochloride (DAPI 2HCl) is a fluorescent dye that binds strongly to adenine–thymine-rich regions in DNA.
In vitro
In vitro

DAPI associates with the minor groove of double-stranded DNA, with a preference for the adenine-thymine clusters. Cells must be permeabilized and/or fixed for DAPI to enter the cell and to bind DNA. Fluorescence increases approximately 20-fold when DAPI is bound to double-stranded DNA. <sup><a class="sref" href="#s_ref">[1]</a></sup>

Chemical Information & Solubility

Molecular Weight 350.25 Formula

C16H15N5.2ClH
CAS No. 28718-90-3 SDF --
Smiles Cl.Cl.NC(=N)C1=CC=C(C=C1)C2=CC3=CC=C(C=C3[NH]2)C(N)=N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 70 mg/mL ( (199.85 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 10 mg/mL

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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