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Hematoxylin

Synonyms: Hydroxybrazilin

Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.

Hematoxylin Chemical Structure

Hematoxylin Chemical Structure

CAS No. 517-28-2

Purity & Quality Control

Hematoxylin Related Products

Biological Activity

Description Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.
In vitro
In vitro

1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change.
2. Hydrate the samples as follows.
i. Transfer the slides through three changes of 100% ethanol for 2 min per change.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer to 70% ethanol for 2 min.
iv. Rinse the slides in running tap water at room temperature for at least 2 min.
3. Stain the samples in Hematoxylin solution for 3 min.
4. Place the slides under running tap water at room temperature for at least 5 min.
5. Stain the samples in working eosin Y solution for 2 min.
6. Dehydrate the samples as follows.
i. Dip the slides in 95% ethanol about 20 times.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer through two changes of 100% ethanol for 2 min per change.
7. Clear the samples in three changes of xylene for 2 min per change.
8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT04793230 Completed
Dacryocystitis; Chronic
Al-Azhar University
 January 1 2019 --
NCT03483935 Completed
Actinic Keratoses|Precancerous Skin Lesion
University of Dundee|Emblation Limited
 March 7 2018 Not Applicable

Chemical Information & Solubility

Molecular Weight 302.28 Formula

C16H14O6
CAS No. 517-28-2 SDF Download Hematoxylin SDF
Smiles C1C2=CC(=C(C=C2C3C1(COC4=C3C=CC(=C4O)O)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 61 mg/mL ( (201.79 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 61 mg/mL

Ethanol : 61 mg/mL


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In vivo
Batch:

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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

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