Hematoxylin

Catalog No.S2384 Batch:S238401

Technical Data

Formula	C₁₆H₁₄O₆
Molecular Weight	302.28	CAS No.	517-28-2
Solubility (25°C)*	In vitro	DMSO	61 mg/mL (201.79 mM)
		Water	61 mg/mL (201.79 mM)
		Ethanol	61 mg/mL (201.79 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.
In vitro	1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change. 2. Hydrate the samples as follows. i. Transfer the slides through three changes of 100% ethanol for 2 min per change. ii. Transfer to 95% ethanol for 2 min. iii. Transfer to 70% ethanol for 2 min. iv. Rinse the slides in running tap water at room temperature for at least 2 min. 3. Stain the samples in Hematoxylin solution for 3 min. 4. Place the slides under running tap water at room temperature for at least 5 min. 5. Stain the samples in working eosin Y solution for 2 min. 6. Dehydrate the samples as follows. i. Dip the slides in 95% ethanol about 20 times. ii. Transfer to 95% ethanol for 2 min. iii. Transfer through two changes of 100% ethanol for 2 min per change. 7. Clear the samples in three changes of xylene for 2 min per change. 8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.

Description

Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.

In vitro

1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change.
2. Hydrate the samples as follows.
i. Transfer the slides through three changes of 100% ethanol for 2 min per change.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer to 70% ethanol for 2 min.
iv. Rinse the slides in running tap water at room temperature for at least 2 min.
3. Stain the samples in Hematoxylin solution for 3 min.
4. Place the slides under running tap water at room temperature for at least 5 min.
5. Stain the samples in working eosin Y solution for 2 min.
6. Dehydrate the samples as follows.
i. Dip the slides in 95% ethanol about 20 times.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer through two changes of 100% ethanol for 2 min per change.
7. Clear the samples in three changes of xylene for 2 min per change.
8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.

Protocol (from reference)

References

[1] Godwin A, J Pharm Clin Sci, 2011, 1, 24-34.

Selleck's Hematoxylin has been cited by 5 publications

Telmisartan inhibits bladder smooth muscle fibrosis in neurogenic bladder rats [ Exp Ther Med, 2022, 23(3):216]	PubMed: 35126719
Divergent and Overlapping Roles for Selected Phytochemicals in the Regulation of Pathological Cardiac Hypertrophy [ Molecules, 2021, 26(5)1210]	PubMed: 33668293
An antiviral drug screening system for enterovirus 71 based on an improved plaque assay: A potential high-throughput method [ J Med Virol, 2019, 91(8):1440-1447]	PubMed: 30900754
Homeobox B8 Targets Sterile Alpha Motif Domain-Containing Protein 9 and Drives Glioma Progression. [ Neurosci Bull, 2019, 10.1007/s12264-019-00436-y]	PubMed: 31646435
Effect of TW37 on the growth of H1975 EGFR‑TKI‑resistant lung cancer cells and its underlying mechanisms. [ Mol Med Rep, 2018, 17(2):2509-2514]	PubMed: 29207200

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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