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Dihydroethidium

Name: Dihydroethidium
Brand: Selleck Chemicals
SKU: S6792
Rating: 5 (4 reviews)

Synonyms: DHE, HE, Hydroethidine, PD-MY 003

Catalog No.S6792 Purity:98.16%

Dihydroethidium (DHE, HE, Hydroethidine, PD-MY 003) is a cell-permeable blue fluorogenic probe used for detecting intracellular superoxide radical anion.

Dihydroethidium Chemical Structure

CAS No. 104821-25-2

Purity & Quality Control

Batch: S679201 DMSO]63 mg/mL]false]Ethanol]2 mg/mL]false]Water]Insoluble]false Purity: 98.16%

98.16

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Biological Activity

Description	Dihydroethidium (DHE, HE, Hydroethidine, PD-MY 003) is a cell-permeable blue fluorogenic probe used for detecting intracellular superoxide radical anion.

In vitro
In vitro	1.1 Preparation of the stock solution Dissolve 1 mg of Dihydroethidium in 0.31 mL of DMSO to obtain 10 mM of Dihydroethidium. Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Dihydroethidium working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of Dihydroethidium working solution. Note: Please adjust the concentration of Dihydroethidium working solution according to the actual situation. Cell staining 2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of Dihydroethidium working solution, and then incubate at room temperature for 30 minutes. 2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT05638763	Recruiting	Chronic Myeloid Leukemia Chronic Phase	Hospital Universitario Dr. Jose E. Gonzalez\|Fernando De la Garza Salazar	November 2024	Phase 2
NCT06385028	Not yet recruiting	Osteoarthritis	Luxembourg Institute of Health\|Œuvre Nationale de Secours Grande Duchesse Charlotte	October 1 2024	Not Applicable

References

[1] Jacek Zielonka, B Kalyanaraman. Free Radic Biol Med. 2010 Apr 15;48(8):983-1001.

Chemical Information & Solubility

Molecular Weight	315.41	Formula	C₂₁H₂₁N₃
CAS No.	104821-25-2	SDF	--
Smiles	CCN1C(C2=CC=CC=C2)C3=C(C=CC(=C3)N)C4=C1C=C(N)C=C4
Storage (From the date of receipt)	3 years -20°C(in the dark) powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C(in the dark) powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 63 mg/mL ( (199.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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