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Propidium Iodide

Synonyms: PI

Propidium iodide (PI) is a red-fluorescent DNA stain which penetrates only damaged cellular membranes. Propidium iodide (PI) is described for use in the determination of cell viability in cell suspension.

Propidium Iodide Chemical Structure

Propidium Iodide Chemical Structure

CAS No. 25535-16-4

Purity & Quality Control

Batch: S687401 DMSO]100 mg/mL]false]Water]10 mg/mL]false]Ethanol]1 mg/mL]false Purity: 99.79%
99.79

Propidium Iodide Related Products

Biological Activity

Description Propidium iodide (PI) is a red-fluorescent DNA stain which penetrates only damaged cellular membranes. Propidium iodide (PI) is described for use in the determination of cell viability in cell suspension.
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Chemical Information & Solubility

Molecular Weight 668.39 Formula

C27H34N4.2I
CAS No. 25535-16-4 SDF --
Smiles CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (149.61 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 10 mg/mL

Ethanol : 1 mg/mL


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO % % Tween 80 % ddH2O
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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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