Home Metabolism Transferase inhibitor - SAM MTase inhibitor ADOX (Adenosine Dialdehyde) For research use only.

ADOX (Adenosine Dialdehyde)

ADOX (Adenosine Dialdehyde) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.

ADOX (Adenosine Dialdehyde) Chemical Structure

ADOX (Adenosine Dialdehyde) Chemical Structure

CAS No. 34240-05-6

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ADOX (Adenosine Dialdehyde) Related Products

Signaling Pathway

Transferase Signaling Pathways

Transferase Signaling Pathway

Biological Activity

Description ADOX (Adenosine Dialdehyde) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.
Targets
AdoHcy hydrolase [1]
(Cell-free)
40 nM
In vitro
In vitro Adenosine dialdehyde (AdOx), an indirect inhibitor that can be incorporated by cells. AdOx inhibits S-adenosyl-L-homocystein hydrolase, resulting in the accumulation of S-adenosyl-L-homo cystein (Adoicy), a product inhibitor of methyltransferases that utilize S-adenosyl-L-methionine (AdoMet) as the methyl group donor[2]. AdOx inhibited the Tax-activated NF-κB pathway, resulting in reactivation of p53 and induction of p53 target genes. Analysis of the NF-κB pathway demonstrated that AdOx treatment resulted in degradation of the IκB kinase complex and inhibition of NF-κB through stabilization of the NF-κB inhibitor IκBα. AdOx induced G2/M cell cycle arrest and cell death in HTLV-1-transformed but not control lymphocytes[4].
Cell Research Cell lines Hela cells
Concentrations 0, 10, 20 or 40 μM
Incubation Time 24 and 48 h
Method

Hela cells are grown in MEM medium supplemented with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. Treatment of cells with AdOx for various time periods was performed. The cells are harvested and washed with phosphate-buffered saline, resuspended in buffer A (phosphate-buffered saline with 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, 0.5% Triton X-100 and Complete protease inhibitor cocktail).
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-Tau / Tau AUF1 23943618
In Vivo
In vivo AdOx exerts a potent inhibitory effect on the in situ growth of established murine neuroblastoma (MNB) tumors, prolongs the life span of tumor bearing mice, and does not suppress hematopoiesis when administered by steady state infusion. AdOx inhibits the replication of L1210 leukemia cells and increased life span approximately 40% when administered i.p. at a dose of 20 mg/kg/day until death[3].
Animal Research Animal Models Murine Neuroblastoma Tumor Model (adult male A/J mice)
Dosages 1.5 to 2.5 mg/kg/day
Administration s.c.

Chemical Information & Solubility

Molecular Weight 265.23 Formula

C10H11N5O4
CAS No. 34240-05-6 SDF Download ADOX (Adenosine Dialdehyde) SDF
Smiles C1=NC(=C2C(=N1)N(C=N2)C(C=O)OC(CO)C=O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 19 mg/mL ( (71.63 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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