Home Metabolism Transferase inhibitor OSMI-1 For research use only.

OSMI-1

OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor with IC50 of 2.7 μM for full length human OGT (ncOGT).

OSMI-1 Chemical Structure

OSMI-1 Chemical Structure

CAS No. 1681056-61-0

Purity & Quality Control

OSMI-1 Related Products

Signaling Pathway

Transferase Signaling Pathways

Transferase Signaling Pathway

Biological Activity

Description OSMI-1 is a cell-permeable O-GlcNAc transferase (OGT) inhibitor with IC50 of 2.7 μM for full length human OGT (ncOGT).
Targets
ncOGT [1]
(Cell-free assay)
2.7 μM
In vitro
In vitro

OSMI-1 is cell-permeable and inhibits protein O-GlcNAcylation in several mammalian cell lines without qualitatively altering cell surface N- or O-linked glycans.[1]
Cell Research Cell lines CHO cells
Concentrations 10 μM, 25 μM, 50 μM, 100 μM
Incubation Time 24 h
Method

CHO cells grown to 50-60% confluency are treated with OSMI-1, at the indicated concentrations for 24 hours. After treatment, cells are harvested and cell lysates are immunoblotted with the indicated antibodies.
In Vivo
In vivo

OSMI-1 acts as a potential chemosensitizer by enhancing DOX-induced cell death. The combination of DOX and OSMI-1 stimulates cell death, dramatically reducing cell proliferation and tumor growth in vivo using a HepG2 xenograft mouse model.[2]
Animal Research Animal Models 5-week-old female BALB/c-Foxn1nu athymic nude mice
Dosages 1 mg/kg, 5 mg/kg
Administration IV

Chemical Information & Solubility

Molecular Weight 563.64 Formula

C28H25N3O6S2
CAS No. 1681056-61-0 SDF --
Smiles COC1=CC=CC=C1C(N[S](=O)(=O)C2=CC3=C(NC(=O)C=C3)C=C2)C(=O)N(CC4=CC=CO4)CC5=CC=CS5
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (177.41 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
Batch:

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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