Home Metabolism Transferase inhibitor SGN-2FF For research use only.

SGN-2FF

Synonyms: 2-fluorofucose

SGN‐2FF (2-fluorofucose) is an orally bioavailable small‐molecule inhibitor of fucosyltransferase that demonstrated encouraging preclinical antitumor activity in mouse models.

SGN-2FF Chemical Structure

SGN-2FF Chemical Structure

CAS No. 2089647-47-0

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SGN-2FF Related Products

Signaling Pathway

Transferase Signaling Pathways

Transferase Signaling Pathway

Biological Activity

Description

SGN‐2FF (2-fluorofucose) is an orally bioavailable small‐molecule inhibitor of fucosyltransferase that demonstrated encouraging preclinical antitumor activity in mouse models.
Targets
fucosyltransferase [1]
In vitro
In vitro

SGN‐2FF can fully inhibit the fucosylation of the h1F6 mAb at the lowest screening concentration in CHO cells treated with SGN‐2FF. The functional effect of inhibiting fucosylation of LS174T cells with SGN‐2FF is apparent from its diminished adherence to E-selectin–coated plates.[2]
Cell Research Cell lines human LS174T colorectal cells, CHO cells
Concentrations 100 μM
Incubation Time 10 days
Method

LS174T cells are cultured in Eagle MEM with 10% FBS with or without 100 μM SGN‐2FF for 10 d. LS174T xenografts from SGN‐2FF-treated or untreated mice are disaggregated by treatment with collagenase, and loose cells are collected. Cells from either source are washed with PBS solution containing 2% FBS and 0.02% sodium azide and stained with biotinylated LCA, cBR96-Alexa Fluor 488, or SSEAI–Alexa Fluor 488 (1 h on ice). Cells are washed and resuspended in PBS solution/FBS/sodium azide at 4 °C. The lectin-stained cells are treated with FITC-conjugated avidin D (30 min on ice), washed, and then analyzes by flow cytometry.
In Vivo
In vivo

In mice, SGN‐2FF inhibits fucosylation of endogenously produced antibodies, tumor xenograft membranes, and neutrophil adhesion glycans. SGN‐2FF treatment affords complete protection from tumor engraftment in a syngeneic tumor vaccine model, inhibits neutrophil extravasation, and delays the outgrowth of tumor xenografts in immune-deficient mice. [2]
Animal Research Animal Models Mouse
Dosages 1 mM, 10 mM, 100 mM
Administration i.g.

Chemical Information & Solubility

Molecular Weight 166.15 Formula

C6H11FO4
CAS No. 2089647-47-0 SDF --
Smiles CC1OC(O)C(F)C(O)C1O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 33 mg/mL ( (198.61 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 33 mg/mL

Ethanol : 17 mg/mL


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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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