Home Metabolism Transferase inhibitor 2-BP (2-Bromohexadecanoic acid) For research use only.

2-BP (2-Bromohexadecanoic acid)

Synonyms: 2-bromopalmitate

2-BP (2-Bromohexadecanoic acid) is a nonmetabolisable analogue of palmitate and acts as a palmitoylation inhibitor, which inhibits multiple palmitoyl-acyl transferase (PAT) activity towards MyrGCK(NBD) and FarnCNRas(NBD) with identical IC50s of approx. 4 µM. 2-Bromohexadecanoic acid can directly and irreversibly inhibit the palmitoyltransferase activity of all DHHC (Asp-His-His-Cys) proteins.

2-BP (2-Bromohexadecanoic acid) Chemical Structure

2-BP (2-Bromohexadecanoic acid) Chemical Structure

CAS No. 18263-25-7

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2-BP (2-Bromohexadecanoic acid) Related Products

Signaling Pathway

Transferase Signaling Pathways

Transferase Signaling Pathway

Biological Activity

Description 2-BP (2-Bromohexadecanoic acid) is a nonmetabolisable analogue of palmitate and acts as a palmitoylation inhibitor, which inhibits multiple palmitoyl-acyl transferase (PAT) activity towards MyrGCK(NBD) and FarnCNRas(NBD) with identical IC50s of approx. 4 µM. 2-Bromohexadecanoic acid can directly and irreversibly inhibit the palmitoyltransferase activity of all DHHC (Asp-His-His-Cys) proteins.
Targets
palmitoyltransferase [1] multiple palmitoyl-acyl transferase (PAT) [2]
4 µM
In vitro
In vitro

2-Bromohexadecanoic acid induces the interactions between GSDME-N and GSDME-C, and inhibits the GSDME-C palmitoylation, exhibiting chemotherapy induced pyroptosis.[1]
Cell Research Cell lines Hela cells
Concentrations 50 μM
Incubation Time 3, 6, 9, 12 h
Method

Cells are pre-seeded overnight until cell density reached ~60%. The culture medium is switched to fresh medium containing relevant drugs and incubated for a period of time as indicated. After 2-BP and TNFα+CHX treatment, Hela cells are collected at the indicated time points and stained with FITC-Annexin V and PI, and subjected to Hoechst 33342 and PI double staining. The culture supernatants are collected to measure the percentage of LDH release.

Chemical Information & Solubility

Molecular Weight 335.32 Formula

C16H31BrO2
CAS No. 18263-25-7 SDF --
Smiles CCCCCCCCCCCCCCC(Br)C(O)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (199.8 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 67 mg/mL

Water : Insoluble


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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