2-BP (2-Bromohexadecanoic acid)

Catalog No.E0120 Batch:E012002

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Technical Data

Formula

C16H31BrO2
Molecular Weight 335.32 CAS No. 18263-25-7
Solubility (25°C)* In vitro DMSO 67 mg/mL (199.8 mM)
Ethanol 67 mg/mL (199.8 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description 2-BP (2-Bromohexadecanoic acid) is a nonmetabolisable analogue of palmitate and acts as a palmitoylation inhibitor, which inhibits multiple palmitoyl-acyl transferase (PAT) activity towards MyrGCK(NBD) and FarnCNRas(NBD) with identical IC50s of approx. 4 µM. 2-Bromohexadecanoic acid can directly and irreversibly inhibit the palmitoyltransferase activity of all DHHC (Asp-His-His-Cys) proteins.
Targets
palmitoyltransferase [1] multiple palmitoyl-acyl transferase (PAT) [2]
4 µM
In vitro

2-Bromohexadecanoic acid induces the interactions between GSDME-N and GSDME-C, and inhibits the GSDME-C palmitoylation, exhibiting chemotherapy induced pyroptosis.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Hela cells

  • Concentrations

    50 μM

  • Incubation Time

    3, 6, 9, 12 h

  • Method

    Cells are pre-seeded overnight until cell density reached ~60%. The culture medium is switched to fresh medium containing relevant drugs and incubated for a period of time as indicated. After 2-BP and TNFα+CHX treatment, Hela cells are collected at the indicated time points and stained with FITC-Annexin V and PI, and subjected to Hoechst 33342 and PI double staining. The culture supernatants are collected to measure the percentage of LDH release.

Selleck's 2-BP (2-Bromohexadecanoic acid) has been cited by 3 publications

CLDN6 inhibits breast cancer growth and metastasis through SREBP1-mediated RAS palmitoylation [ Cell Mol Biol Lett, 2024, 29(1):112] PubMed: 39169280
Hepatitis E virus ORF3 protein hijacking thioredoxin domain-containing protein 5 (TXNDC5) for its stability to promote viral particle release [ J Virol, 2024, 98(4):e0164923] PubMed: 38548704
ZDHHC2-Mediated AGK Palmitoylation Activates AKT-mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell Carcinoma [ Cancer Res, 2023, 83(12):2034-2051] PubMed: 37078777

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.