ADOX (Adenosine Dialdehyde)

Catalog No.S8608 Batch:S860802

Technical Data

Formula	C₁₀H₁₁N₅O₄
Molecular Weight	265.23	CAS No.	34240-05-6
Solubility (25°C)*	In vitro	DMSO	19 mg/mL (71.63 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

ADOX (Adenosine Dialdehyde) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.

Targets

AdoHcy hydrolase ^[1]
(Cell-free)

40 nM

In vitro

Adenosine dialdehyde (AdOx), an indirect inhibitor that can be incorporated by cells. AdOx inhibits S-adenosyl-L-homocystein hydrolase, resulting in the accumulation of S-adenosyl-L-homo cystein (Adoicy), a product inhibitor of methyltransferases that utilize S-adenosyl-L-methionine (AdoMet) as the methyl group donor^[2]. AdOx inhibited the Tax-activated NF-κB pathway, resulting in reactivation of p53 and induction of p53 target genes. Analysis of the NF-κB pathway demonstrated that AdOx treatment resulted in degradation of the IκB kinase complex and inhibition of NF-κB through stabilization of the NF-κB inhibitor IκBα. AdOx induced G2/M cell cycle arrest and cell death in HTLV-1-transformed but not control lymphocytes^[4].

In vivo

AdOx exerts a potent inhibitory effect on the in situ growth of established murine neuroblastoma (MNB) tumors, prolongs the life span of tumor bearing mice, and does not suppress hematopoiesis when administered by steady state infusion. AdOx inhibits the replication of L1210 leukemia cells and increased life span approximately 40% when administered i.p. at a dose of 20 mg/kg/day until death^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Hela cells Concentrations 0, 10, 20 or 40 μM Incubation Time 24 and 48 h Method Hela cells are grown in MEM medium supplemented with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. Treatment of cells with AdOx for various time periods was performed. The cells are harvested and washed with phosphate-buffered saline, resuspended in buffer A (phosphate-buffered saline with 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, 0.5% Triton X-100 and Complete protease inhibitor cocktail).
Animal Study:^{^[3]}	Animal Models Murine Neuroblastoma Tumor Model (adult male A/J mice) Dosages 1.5 to 2.5 mg/kg/day Administration s.c.

Cell Assay:^{^[1]}

Cell lines
Hela cells
Concentrations
0, 10, 20 or 40 μM
Incubation Time
24 and 48 h
Method
Hela cells are grown in MEM medium supplemented with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. Treatment of cells with AdOx for various time periods was performed. The cells are harvested and washed with phosphate-buffered saline, resuspended in buffer A (phosphate-buffered saline with 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, 0.5% Triton X-100 and Complete protease inhibitor cocktail).

Animal Study:^{^[3]}

Animal Models
Murine Neuroblastoma Tumor Model (adult male A/J mice)
Dosages
1.5 to 2.5 mg/kg/day
Administration
s.c.

References

[4] Arindam Dasgupta, et al. J Virol. 2008, 82(1): 49-59.

+ Expand

Selleck's ADOX (Adenosine Dialdehyde) has been cited by 9 publications

Methionine-SAM metabolism-dependent ubiquinone synthesis is crucial for ROS accumulation in ferroptosis induction [ Nat Commun, 2024, 15(1):8971]	PubMed: 39420002
MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression [ EMBO J, 2023, e114558.]	PubMed: 37905571
Dynamic assembly of the mRNA m6A methyltransferase complex is regulated by METTL3 phase separation [ PLoS Biol, 2022, 20(2):e3001535]	PubMed: 35143475
A biochemical reconstitution approach to identify autophagy receptors for aggrephagy in mammalian cells [ STAR Protoc, 2022, 3(3):101662]	PubMed: 36097383
AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner [ Autophagy, 2021, 1-11]	PubMed: 33993848
AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner [ Autophagy, 2021, 1-11]	PubMed: 33993848
ESRP1 regulates alternative splicing of CARM1 to sensitize small cell lung cancer cells to chemotherapy by inhibiting TGF-β/Smad signaling [ Aging (Albany NY), 2021, 13(3):3554-3572]	PubMed: 33495408
Hypoxia stimulates SUMOylation-dependent stabilization of KDM5B [ Research Square, 2021, 10.21203/rs.3.rs-620216/v1]	PubMed: None
Inhibition of S-adenosyl-L-homocysteine hydrolase alleviates alloimmune response by down-regulating CD4+ T-cell activation in a mouse heart transplantation model [ Ann Transl Med, 2020, 8(23):1582]	PubMed: 33437781

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SHIPPING AND STORAGE
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