ADOX (Adenosine Dialdehyde)

Catalog No.S8608 Batch:S860801

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Technical Data

Formula

C10H11N5O4
Molecular Weight 265.23 CAS No. 34240-05-6
Solubility (25°C)* In vitro DMSO 13.2 mg/mL (49.76 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
2.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description ADOX (Adenosine Dialdehyde) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.
Targets
AdoHcy hydrolase [1]
(Cell-free)
40 nM
In vitro Adenosine dialdehyde (AdOx), an indirect inhibitor that can be incorporated by cells. AdOx inhibits S-adenosyl-L-homocystein hydrolase, resulting in the accumulation of S-adenosyl-L-homo cystein (Adoicy), a product inhibitor of methyltransferases that utilize S-adenosyl-L-methionine (AdoMet) as the methyl group donor[2]. AdOx inhibited the Tax-activated NF-κB pathway, resulting in reactivation of p53 and induction of p53 target genes. Analysis of the NF-κB pathway demonstrated that AdOx treatment resulted in degradation of the IκB kinase complex and inhibition of NF-κB through stabilization of the NF-κB inhibitor IκBα. AdOx induced G2/M cell cycle arrest and cell death in HTLV-1-transformed but not control lymphocytes[4].
In vivo AdOx exerts a potent inhibitory effect on the in situ growth of established murine neuroblastoma (MNB) tumors, prolongs the life span of tumor bearing mice, and does not suppress hematopoiesis when administered by steady state infusion. AdOx inhibits the replication of L1210 leukemia cells and increased life span approximately 40% when administered i.p. at a dose of 20 mg/kg/day until death[3].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Hela cells

  • Concentrations

    0, 10, 20 or 40 μM

  • Incubation Time

    24 and 48 h

  • Method

    Hela cells are grown in MEM medium supplemented with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. Treatment of cells with AdOx for various time periods was performed. The cells are harvested and washed with phosphate-buffered saline, resuspended in buffer A (phosphate-buffered saline with 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, 0.5% Triton X-100 and Complete protease inhibitor cocktail).
Animal Study:

[3]
  • Animal Models

    Murine Neuroblastoma Tumor Model (adult male A/J mice)

  • Dosages

    1.5 to 2.5 mg/kg/day

  • Administration

    s.c.

Selleck's ADOX (Adenosine Dialdehyde) has been cited by 9 publications

Methionine-SAM metabolism-dependent ubiquinone synthesis is crucial for ROS accumulation in ferroptosis induction [ Nat Commun, 2024, 15(1):8971] PubMed: 39420002
MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression [ EMBO J, 2023, e114558.] PubMed: 37905571
Dynamic assembly of the mRNA m6A methyltransferase complex is regulated by METTL3 phase separation [ PLoS Biol, 2022, 20(2):e3001535] PubMed: 35143475
A biochemical reconstitution approach to identify autophagy receptors for aggrephagy in mammalian cells [ STAR Protoc, 2022, 3(3):101662] PubMed: 36097383
AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner [ Autophagy, 2021, 1-11] PubMed: 33993848
AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner [ Autophagy, 2021, 1-11] PubMed: 33993848
ESRP1 regulates alternative splicing of CARM1 to sensitize small cell lung cancer cells to chemotherapy by inhibiting TGF-β/Smad signaling [ Aging (Albany NY), 2021, 13(3):3554-3572] PubMed: 33495408
Hypoxia stimulates SUMOylation-dependent stabilization of KDM5B [ Research Square, 2021, 10.21203/rs.3.rs-620216/v1] PubMed: None
Inhibition of S-adenosyl-L-homocysteine hydrolase alleviates alloimmune response by down-regulating CD4+ T-cell activation in a mouse heart transplantation model [ Ann Transl Med, 2020, 8(23):1582] PubMed: 33437781

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.