HT29 |
Growth Inhibition Assay |
0-10 μM |
0-4 d |
|
inhibits cell growth in both time- and dose-dependent manner |
26702784 |
HepG2 |
Growth Inhibition Assay |
0-10 μM |
0-4 d |
|
inhibits cell growth in both time- and dose-dependent manner |
26702784 |
HT29 |
Function Assay |
50 nM |
24-72 h |
|
induced activation of caspase 3 after 48 h |
26702784 |
HepG2 |
Function Assay |
50 nM |
24-72 h |
|
induced activation of caspase 3 after 24 h |
26702784 |
HCC827 |
Growth Inhibition Assay |
5/7.5/10 nM |
72 h |
DMSO |
enhances the antiproliferative effect of erlotinib |
26675484 |
A549 |
Growth Inhibition Assay |
10/15/20 nM |
72 h |
DMSO |
enhances the antiproliferative effect of erlotinib |
26675484 |
NCI-H460 |
Growth Inhibition Assay |
10/20/30 nM |
72 h |
DMSO |
enhances the antiproliferative effect of erlotinib |
26675484 |
J89GFP |
Growth Inhibition Assay |
|
|
DMSO |
EC50=49.85 ± 12.65 nM |
26563568 |
THP89GFP |
Growth Inhibition Assay |
|
|
DMSO |
EC50=19.34 ± 6.43 nM |
26563568 |
SK-NEP-1 |
Growth Inhibition Assay |
0.01–10.0 μM |
24 h |
DMSO |
IC50=76.34 nM |
26176219 |
G401 |
Growth Inhibition Assay |
0.01–10.0 μM |
24 h |
DMSO |
IC50=143.02 nM |
26176219 |
SK-NEP-1 |
Cell Viability Assay |
50 nM |
1–4 d |
DMSO |
reduces cell survival in a time dependent manner |
26176219 |
G401 |
Cell Viability Assay |
50 nM |
1–4 d |
DMSO |
reduces cell survival in a time dependent manner |
26176219 |
SK-NEP-1 |
Apoptosis Assay |
50/100 nM |
24 h |
DMSO |
induces cell apoptosis in a dose-dependent manner |
26176219 |
G401 |
Apoptosis Assay |
50/100 nM |
24 h |
DMSO |
induces cell apoptosis in a dose-dependent manner |
26176219 |
SK-NEP-1 |
Function Assay |
50/100 nM |
24 h |
DMSO |
shows the induction of DNA fragmentation |
26176219 |
G401 |
Function Assay |
50/100 nM |
24 h |
DMSO |
shows the induction of DNA fragmentation |
26176219 |
SK-NEP-1 |
Function Assay |
50/100 nM |
24 h |
DMSO |
induces cell cycle disorder |
26176219 |
G401 |
Function Assay |
50/100 nM |
24 h |
DMSO |
induces cell cycle disorder |
26176219 |
RPMI 8226 |
Cell Survival Assay |
2/4/6 nM |
48 h |
|
induces a significant decrease in the cell growth |
26000292 |
OPM2 |
Cell Survival Assay |
2/4/6 nM |
48 h |
|
induces a significant decrease in the cell growth |
26000292 |
U266 |
Cell Survival Assay |
2/4/6 nM |
48 h |
|
induces a significant decrease in the cell growth |
26000292 |
H929 |
Cell Survival Assay |
2/4/6 nM |
48 h |
|
induces a significant decrease in the cell growth |
26000292 |
RPMI 8226 |
Apoptosis Assay |
4 nM |
24/48 h |
|
induces cell apoptosis in a time-dependent manner |
26000292 |
HCC827 |
Growth Inhibition Assay |
10 nM |
48 h |
DMSO |
enhances cisplatin sensitivity |
25944617 |
NCI-H23 |
Growth Inhibition Assay |
10 nM |
48 h |
DMSO |
enhances cisplatin sensitivity |
25944617 |
AML3 |
Function Assay |
0-1 μM |
24 h |
|
induces DNA fragmentation in a dose-dependent manner |
25612941 |
ML-1 |
Function Assay |
0-1 μM |
24 h |
|
induces DNA fragmentation in a dose-dependent manner |
25612941 |
RPMI-8226vr10 |
Function Assay |
0-1 μM |
24 h |
|
induces DNA fragmentation in a dose-dependent manner |
25612941 |
ML-1 |
Function Assay |
1 μM |
24 h |
|
increases caspase-3 activity 4-fold |
25612941 |
RPMI-8226vr10 |
Function Assay |
1 μM |
24 h |
|
increases caspase-3 activity 2.5-fold |
25612941 |
SK-N-BE (2) |
Growth Inhibition Assay |
|
24 h |
|
IC50=104.0 ± 7.8 nM |
25308916 |
SK-N-BE (2), PAN MK |
Growth Inhibition Assay |
|
24 h |
|
IC50=104.0 ± 7.8 nM |
25308916 |
SK-N-BE (2), MK PAN |
Growth Inhibition Assay |
|
24 h |
|
IC50=382.0 ± 43.2 nM |
25308916 |
SK-N-AS |
Growth Inhibition Assay |
|
24 h |
|
IC50=37.1 ± 2.4 nM |
25308916 |
SK-N-DZ |
Growth Inhibition Assay |
|
24 h |
|
IC50=17.1 ± 0.4 nM |
25308916 |
Caki-1 |
Growth Inhibition Assay |
10/25/50 nM |
48 h |
|
inhibits cell growth in a dose dependent manner synergistically with ritonavir |
25279191 |
ACHN |
Growth Inhibition Assay |
10/25/50 nM |
48 h |
|
inhibits cell growth in a dose dependent manner synergistically with ritonavir |
25279191 |
769-P |
Growth Inhibition Assay |
10/25/50 nM |
48 h |
|
inhibits cell growth in a dose dependent manner synergistically with ritonavir |
25279191 |
786-O |
Growth Inhibition Assay |
10/25/50 nM |
48 h |
|
inhibits cell growth in a dose dependent manner synergistically with ritonavir |
25279191 |
Caki-1 |
Apoptosis Assay |
50 nM |
48 h |
|
induces cell apoptosis combined ritonavir |
25279191 |
ACHN |
Apoptosis Assay |
50 nM |
48 h |
|
induces cell apoptosis combined ritonavir |
25279191 |
769-P |
Apoptosis Assay |
50 nM |
48 h |
|
induces cell apoptosis combined ritonavir |
25279191 |
786-O |
Apoptosis Assay |
50 nM |
48 h |
|
induces cell apoptosis combined ritonavir |
25279191 |
Caki-1 |
Growth Inhibition Assay |
25/50 nM |
48 h |
DMSO |
inhibits cell growth in a dose dependent manner synergistically with bortezomib |
25176354 |
ACHN |
Growth Inhibition Assay |
25/50 nM |
48 h |
DMSO |
inhibits cell growth in a dose dependent manner synergistically with bortezomib |
25176354 |
769-P |
Growth Inhibition Assay |
25/50 nM |
48 h |
DMSO |
inhibits cell growth in a dose dependent manner synergistically with bortezomib |
25176354 |
Caki-1 |
Colony Formation Assay |
50 nM |
7-14 d |
DMSO |
suppressed colony formation significantly combined with with bortezomib |
25176354 |
ACHN |
Colony Formation Assay |
50 nM |
7-14 d |
DMSO |
suppressed colony formation significantly combined with with bortezomib |
25176354 |
769-P |
Colony Formation Assay |
50 nM |
7-14 d |
DMSO |
suppressed colony formation significantly combined with with bortezomib |
25176354 |
Caki-1 |
Apoptosis Assay |
50 nM |
48 h |
DMSO |
induces cell apoptosis |
25176354 |
ACHN |
Apoptosis Assay |
50 nM |
48 h |
DMSO |
induces cell apoptosis |
25176354 |
769-P |
Apoptosis Assay |
50 nM |
48 h |
DMSO |
induces cell apoptosis |
25176354 |
MDA-MB-231 |
Morphological Crystal Violet (CV) Assay |
10 nM |
3 d |
DMSO |
alters cell morphology |
24810497 |
BT-549 |
Morphological Crystal Violet (CV) Assay |
10 nM |
3 d |
DMSO |
alters cell morphology |
24810497 |
MCF-7 |
Morphological Crystal Violet (CV) Assay |
10 nM |
3 d |
DMSO |
alters cell morphology |
24810497 |
MCF-7 |
Function Assay |
5-50 nM |
24 h |
DMSO |
reduced the level of expression of ERα, PR and FoxA1 |
24366407 |
CTS |
Apoptosis Assay |
0–40 nM |
48 h |
|
induces apoptosis in a dose-dependent manner |
24244429 |
OCI-AML3 |
Apoptosis Assay |
0–40 nM |
48 h |
|
induces apoptosis in a dose-dependent manner |
24244429 |
U937 |
Apoptosis Assay |
0–40 nM |
48 h |
|
induces apoptosis in a dose-dependent manner |
24244429 |
PC3 |
Apoptosis Assay |
0-100 nM |
24/48 h |
|
induces apoptosis in a dose-dependent manner |
24163230 |
PC3-AR |
Apoptosis Assay |
0-100 nM |
24/48 h |
|
induces apoptosis in both time- and dose-dependent manner |
24163230 |
PC3 |
Growth Inhibition Assay |
0-100 nM |
24/48 h |
|
induces accumulation of subG1 population |
24163230 |
PC3-AR |
Growth Inhibition Assay |
0-100 nM |
24/48 h |
|
induces cell cycle arrest in the G2M phase |
24163230 |
PC3 |
Function Assay |
0-100 nM |
24 h |
|
suppresses expression of activated ATM, Akt and Erk1/2 protein |
24163230 |
PC3-AR |
Function Assay |
0-100 nM |
24 h |
|
suppresses expression of activated ATM, Akt and Erk1/2 protein |
24163230 |
OS-RC-2 |
Cell Viability Assay |
0-1000 nM |
24/48/72 h |
DMSO |
decreases cell viability in both time- and dose-dependent manner |
24144737 |
OS-RC-2 |
Growth Inhibition Assay |
50 nM |
48 h |
DMSO |
induces G2/M arrest |
24144737 |
OS-RC-2 |
Apoptosis Assay |
50 nM |
48 h |
DMSO |
induces cell apoptosis |
24144737 |
SK-N-AS |
Growth Inhibition Assay |
0–80 nM |
48 h |
|
IC50=27.4 nM |
24098799 |
SK-N-DZ |
Growth Inhibition Assay |
0–80 nM |
48 h |
|
IC50=21.9 nM |
24098799 |
SK-N-SH |
Growth Inhibition Assay |
0–80 nM |
48 h |
|
IC50=72.3 nM |
24098799 |
SK-N-BE |
Growth Inhibition Assay |
0–80 nM |
48 h |
|
IC50=75.4 nM |
24098799 |
SK-N-AS |
Apoptosis Assay |
0–80 nM |
48 h |
|
potently induced apoptosis in a dose-dependent fashion |
24098799 |
SK-N-DZ |
Apoptosis Assay |
0–80 nM |
48 h |
|
potently induced apoptosis in a dose-dependent fashion |
24098799 |
SK-N-SH |
Apoptosis Assay |
0–40 nM |
48 h |
|
potently induced apoptosis in a dose-dependent fashion |
24098799 |
SK-N-BE |
Apoptosis Assay |
0–40 nM |
48 h |
|
potently induced apoptosis in a dose-dependent fashion |
24098799 |
SK-N-AS |
Function Assay |
0–80 nM |
48 h |
|
induces a dose-dependent cleavage of caspase 3 and PARP |
24098799 |
SK-N-DZ |
Function Assay |
0–80 nM |
48 h |
|
induces a dose-dependent cleavage of caspase 3 and PARP |
24098799 |
SK-N-SH |
Function Assay |
0–40 nM |
48 h |
|
induces a dose-dependent cleavage of caspase 3 and PARP |
24098799 |
SK-N-BE |
Function Assay |
0–40 nM |
48 h |
|
induces a dose-dependent cleavage of caspase 3 and PARP |
24098799 |
HCC-LM3 |
Growth Inhibition Assay |
1-1000 nM |
24/48/72 h |
DMSO |
inhibits cell growth in both time- and dose-dependent manner |
24093956 |
HepG2 |
Growth Inhibition Assay |
1-1000 nM |
24/48/72 h |
DMSO |
inhibits cell growth in both time- and dose-dependent manner |
24093956 |
SMMC-7721 |
Growth Inhibition Assay |
1-1000 nM |
24/48/72 h |
DMSO |
inhibits cell growth in both time- and dose-dependent manner |
24093956 |
HCC-LM3 |
Apoptosis Assay |
50 nM |
48 h |
DMSO |
induces cell apoptosis significantly in a caspase-dependent manner by cleavage of caspases 3, 8 and 9 |
24093956 |
HepG2 |
Apoptosis Assay |
50 nM |
48 h |
DMSO |
induces cell apoptosis significantly in a caspase-dependent manner by cleavage of caspases 3, 8 and 9 |
24093956 |
SMMC-7721 |
Apoptosis Assay |
50 nM |
48 h |
DMSO |
induces cell apoptosis significantly in a caspase-dependent manner by cleavage of caspases 3, 8 and 9 |
24093956 |
HCC-LM3 |
Function Assay |
50/100 nM |
24 h |
DMSO |
decreases the levels of p-STAT3 and p-Akt |
24093956 |
HepG2 |
Function Assay |
50/100 nM |
24 h |
DMSO |
decreases the levels of p-STAT3 and p-Akt |
24093956 |
SMMC-7721 |
Function Assay |
50/100 nM |
24 h |
DMSO |
decreases the levels of p-STAT3 and p-Akt |
24093956 |
HCC-LM3 |
Function Assay |
50/100 nM |
24 h |
DMSO |
downregulates Bcl-xL expression |
24093956 |
HepG2 |
Function Assay |
50/100 nM |
24 h |
DMSO |
downregulates Bcl-xL expression |
24093956 |
SMMC-7721 |
Function Assay |
50/100 nM |
24 h |
DMSO |
downregulates Bcl-xL expression |
24093956 |
FaDu |
Growth Inhibition Assay |
100 nM |
8/10/12 h |
|
displayed a significant and prolonged G2/M arrest at 8 and 12 h post release |
24026482 |
FaDu |
Function Assay |
100 nM |
2/4/8/12 h |
|
induced p21Waf1/Cip1 expression |
24026482 |
PC-3 |
Growth Inhibition Assay |
0-10 μM |
24/48/72 h |
|
inhibits cell growth in both time- and dose-dependent manner |
23991216 |
LNCaP |
Growth Inhibition Assay |
0-5 μM |
24/48/72 h |
|
inhibits cell growth in both time- and dose-dependent manner |
23991216 |
RWPE-1 |
Growth Inhibition Assay |
0-20 μM |
24/48/72 h |
|
inhibits cell growth in both time- and dose-dependent manner |
23991216 |
Capan-1 |
Function Assay |
25/50/100 nM |
8/24/48 h |
DMSO |
downregulated Ron mRNA and protein expression and downstream signaling |
23922886 |
L3.6pl |
Function Assay |
25/50/100 nM |
8/24/48 h |
DMSO |
downregulated Ron mRNA and protein expression and downstream signaling |
23922886 |
CFPAC-1 |
Function Assay |
25/50/100 nM |
8/24/48 h |
DMSO |
downregulated Ron mRNA and protein expression and downstream signaling |
23922886 |
Capan-1 |
Growth Inhibition Assay |
25/50/100 nM |
48 h |
DMSO |
reduces cell growth in a dose-dependent manner |
23922886 |
L3.6pl |
Growth Inhibition Assay |
25/50/100 nM |
48 h |
DMSO |
reduces cell growth in a dose-dependent manner |
23922886 |
CFPAC-1 |
Growth Inhibition Assay |
25/50/100 nM |
48 h |
DMSO |
reduces cell growth in a dose-dependent manner |
23922886 |
Capan-1 |
Apoptosis Assay |
25/50/100 nM |
48 h |
DMSO |
induces cell growth in a dose-dependent manner |
23922886 |
L3.6pl |
Apoptosis Assay |
25/50/100 nM |
48 h |
DMSO |
induces cell growth in a dose-dependent manner |
23922886 |
CFPAC-1 |
Apoptosis Assay |
25/50/100 nM |
48 h |
DMSO |
induces cell growth in a dose-dependent manner |
23922886 |
HN22 |
Growth Inhibition Assay |
0-20 nM |
24/48 h |
DMSO |
inhibits cell viability in both time- and dose- dependent manner |
23877235 |
HSC4 |
Growth Inhibition Assay |
0-20 nM |
24/48 h |
DMSO |
inhibits cell viability in both time- and dose- dependent manner |
23877235 |
HN22 |
Apoptosis Assay |
0-20 nM |
48 h |
DMSO |
induces cell apoptosis |
23877235 |
HSC4 |
Apoptosis Assay |
0-20 nM |
48 h |
DMSO |
induces cell apoptosis |
23877235 |
HN22 |
Growth Inhibition Assay |
0-20 nM |
48 h |
DMSO |
induces G1 phase cell cycle arrest |
23877235 |
HSC4 |
Growth Inhibition Assay |
0-20 nM |
48 h |
DMSO |
induces G1 phase cell cycle arrest |
23877235 |
HN22 |
Function Assay |
0-20 nM |
48 h |
DMSO |
suppresses Sp1 expression |
23877235 |
HSC4 |
Function Assay |
0-20 nM |
48 h |
DMSO |
suppresses Sp1 expression |
23877235 |
Cal62 |
Growth Inhibition Assay |
|
|
|
IC50=33 ± 4 nM |
23824064 |
Hth7 |
Growth Inhibition Assay |
|
|
|
IC50=15 ± 2 nM |
23824064 |
Hth83 |
Growth Inhibition Assay |
|
|
|
IC50=34 ± 5 nM |
23824064 |
C643 |
Growth Inhibition Assay |
|
|
|
IC50=71 ± 10 nM |
23824064 |
SW1736 |
Growth Inhibition Assay |
|
|
|
IC50=35 ± 8 nM |
23824064 |
T241 |
Growth Inhibition Assay |
|
|
|
IC50=65 ± 7 nM |
23824064 |
T351 |
Growth Inhibition Assay |
|
|
|
IC50=50 ± 10 nM |
23824064 |
BHP2-7 |
Growth Inhibition Assay |
|
|
|
IC50=37 ± 6 nM |
23824064 |
T238 |
Growth Inhibition Assay |
|
|
|
IC50=1,500 ± 200 nM |
23824064 |
HCT8 |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=12.9 ± 1.9 nM |
23299388 |
H630 |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=12.4 ± 3.1 nM |
23299388 |
cH630 5-FU-res |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=15.5 ± 1.2 nM |
23299388 |
HCT116 |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=10.7 ± 2.2 nM |
23299388 |
HCT116 p53−/− |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=8.6 ± 1.7 nM |
23299388 |
dHCT116 p21−/− |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=5.9 ± 1.3 nM |
23299388 |
HT29 |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=16.3 ± 2.3 nM |
23299388 |
LoVo |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=5.1 ± 0.6 nM |
23299388 |
RKO |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=7.9 ± 2.2 nM |
23299388 |
SW480 |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=17.5 ± 0.8 nM |
23299388 |
eSW620 |
Growth Inhibition Assay |
|
72 h |
DMSO |
IC50=9.1 ± 2.1 nM |
23299388 |
S2 |
Function assay |
|
|
|
Inhibition of Plasmodium falciparum HDAC1 expressed in Drosophila melanogaster S2 cells, IC50 = 0.0018 μM. |
19317450 |
COLO205 |
Antiproliferative assay |
|
96 hrs |
|
Antiproliferative activity against human COLO205 cells after 96 hrs by celltiter 96 assay, IC50 = 0.018 μM. |
21634430 |
PC3 |
Antiproliferative assay |
|
96 hrs |
|
Antiproliferative activity against human PC3 cells after 96 hrs by celltiter 96 assay, IC50 = 0.024 μM. |
21634430 |
A2780 |
Antiproliferative assay |
|
96 hrs |
|
Antiproliferative activity against human A2780 cells after 96 hrs by celltiter 96 assay, IC50 = 0.035 μM. |
21634430 |
HCT116 |
Antiproliferative assay |
|
96 hrs |
|
Antiproliferative activity against human HCT116 cells after 96 hrs by celltiter 96 assay, IC50 = 0.048 μM. |
21634430 |
HEK293 |
Function assay |
|
|
|
Inhibition of HDAC3 (unknown origin) expressed in HEK293 cells using [3H]acetylated human histone H4 peptide as substrate by scintillation counting, IC50 = 0.0021 μM. |
22344701 |
HEK293 |
Function assay |
|
|
|
Inhibition of HDAC1 (unknown origin) expressed in HEK293 cells using [3H]acetylated human histone H4 peptide as substrate by scintillation counting, IC50 = 0.0025 μM. |
22344701 |
HEK293 |
Function assay |
|
|
|
Inhibition of HDAC6 (unknown origin) expressed in HEK293 cells using [3H]acetylated human histone H4 peptide as substrate by scintillation counting, IC50 = 0.011 μM. |
22344701 |
SF21 |
Function assay |
|
|
|
Inhibition of flag-tagged HDAC2 (unknown origin) expressed in SF21 cells using [3H]acetylated human histone H4 peptide as substrate by scintillation counting, IC50 = 0.013 μM. |
22344701 |
HEK293 |
Function assay |
|
|
|
Inhibition of HDAC4 (unknown origin) expressed in HEK293 cells using [3H]acetylated human histone H4 peptide as substrate by scintillation counting, IC50 = 0.2 μM. |
22344701 |
SF9 |
Function assay |
|
|
|
Inhibition of his-strep-tagged HDAC8 (unknown origin) expressed in SF9 cells using [3H]acetylated human histone H4 peptide as substrate by scintillation counting, IC50 = 0.28 μM. |
22344701 |
B16 |
Growth inhibition assay |
|
48 hrs |
|
Growth inhibition of mouse B16 cells incubated for 48 hrs by MTT assay, GI50 = 0.15 μM. |
23009203 |
HeLa |
Function assay |
|
|
|
Inhibition of HDAC in human HeLa cells using Fluor de Lys as substrate by fluorescence assay, IC50 = 0.03 μM. |
23639537 |
HuH7 |
Cytotoxicity assay |
|
3 days |
|
Cytotoxicity against human HuH7 cells assessed as inhibition of cell viability after 3 days by CellTiter 96 assay, CC50 = 0.0035 μM. |
25490700 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of full length C-terminal His/FLAG-tagged human recombinant HDAC1 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured a, IC50 = 0.00126 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of full length C-terminal His-tagged human recombinant HDAC3/NCOR2 (395 to 489 residues) expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substr, IC50 = 0.00227 μM. |
27186676 |
MV4-11 |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human MV4-11 cells assessed as growth inhibition after 24 hrs by MTT assay, IC50 = 0.00297 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of full length human recombinant HDAC2 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured after 1 hr by fluorescence a, IC50 = 0.00328 μM. |
27186676 |
HCT116 |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 24 hrs by MTT assay, IC50 = 0.00336 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of full length human recombinant HDAC6 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured after 1 hr by fluorescence a, IC50 = 0.00416 μM. |
27186676 |
Sf9 |
Inhibition of human |
|
15 mins |
|
Inhibition of human recombinant HDAC10 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured after 1 hr by fluorescence assay, IC50 = 0.00445 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of full length C-terminal His-tagged human recombinant HDAC8 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured after , IC50 = 0.00486 μM. |
27186676 |
A2780S |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human A2780S cells assessed as growth inhibition after 24 hrs by MTT assay, IC50 = 0.00832 μM. |
27186676 |
A2780S |
Function assay |
|
6 hrs |
|
Inhibition of HDAC6 in human A2780S cells assessed as tubulin acetylation incubated for 6 hrs by cytoblot assay, EC50 = 0.15071 μM. |
27186676 |
A2780S |
Function assay |
|
6 hrs |
|
Inhibition of HDAC1/2/3 in human A2780S cells assessed as histone H3 acetylation incubated for 6 hrs by cytoblot assay, EC50 = 0.1695 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of human recombinant HDAC5 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured after 1 hr by fluorescence assay, IC50 = 0.1903 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of N-terminal GST/C-terminal His-tagged human recombinant HDAC4 (627 to 1084 residues) expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrat, IC50 = 0.3378 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of C-terminal His-tagged human recombinant HDAC9 (604 to 1066 residues) expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition meas, IC50 = 0.8878 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of full length human recombinant HDAC11 expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measured after 1 hr by fluorescence , IC50 = 4.112 μM. |
27186676 |
Sf9 |
Function assay |
|
15 mins |
|
Inhibition of N-terminal GST-tagged human recombinant HDAC7 (518 to end residues) expressed in baculovirus infected insect Sf9 cells using Ac-peptide-AMC as substrate assessed as release of AMC preincubated for 15 mins followed by substrate addition measu, IC50 = 4.354 μM. |
27186676 |
Sf9 |
Function assay |
|
|
|
Inhibition of C-terminal His-tagged and C-terminal FLAG-tagged full length human recombinant HDAC1 expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as substrate , IC50 = 0.001 μM. |
27377864 |
Sf9 |
Function assay |
|
60 mins |
|
Inhibition full length human recombinant HDAC2 expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as substrate after 60 mins by fluorescence assay, IC50 = 0.002 μM. |
27377864 |
Sf9 |
Function assay |
|
60 mins |
|
Inhibition of human recombinant HDAC6 expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as substrate after 60 mins by fluorescence assay, IC50 = 0.002 μM. |
27377864 |
Sf9 |
Function assay |
|
60 mins |
|
Inhibition of N-terminal GST-tagged full length human recombinant HDAC5 expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as substrate after 60 mins by fluorescen, IC50 = 0.092 μM. |
27377864 |
Sf9 |
Function assay |
|
60 mins |
|
Inhibition of C-terminal His-tagged full length human recombinant HDAC8 expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as substrate after 60 mins by fluorescen, IC50 = 0.231 μM. |
27377864 |
Sf9 |
Function assay |
|
|
|
Inhibition of N-terminal GST-tagged and C-terminal His-tagged human recombinant HDAC4 (627 to 1084 residues ) expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as, IC50 = 0.373 μM. |
27377864 |
Sf9 |
Function assay |
|
60 mins |
|
Inhibition of C-terminal His-tagged human recombinant HDAC9 (604 to 1066 residues) expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as substrate after 60 mins by, IC50 = 2.68 μM. |
27377864 |
Sf9 |
Function assay |
|
60 mins |
|
Inhibition of N-terminal GST-tagged human recombinant HDAC7 (518 to end residues) expressed in baculovirus coexpressed in fall armyworm Sf9 cells using carboxyfluorescein (FAM)-labeled acetylated/ trifluoroacetylated peptide as substrate after 60 mins by , IC50 = 2.83 μM. |
27377864 |
HEK293 |
Cytotoxicity assay |
|
48 hrs |
|
Cytotoxicity against HEK293 cells after 48 hrs by resazurin assay, IC50 = 0.07 μM. |
28241112 |
NFF |
Cytotoxicity assay |
|
72 hrs |
|
Cytotoxicity against human NFF cells after 72 hrs by SRB assay, IC50 = 0.07 μM. |
28241112 |
HUT78 |
Apoptosis assay |
|
18 hrs |
|
Pro-apoptotic activity in human HUT78 cells after 18 hrs by caspase-Glo 3/7 assay, EC50 = 0.0043 μM. |
30122227 |
NFF |
Cytotoxicity assay |
|
72 hrs |
|
Cytotoxicity against human NFF cells after 72 hrs by sulforhodamine B assay, IC50 = 0.07 μM. |
30245402 |
HEK293 |
Cytotoxicity assay |
|
48 hrs |
|
Cytotoxicity against HEK293 cells after 48 hrs by resazurin dye based assay, IC50 = 0.07 μM. |
30245402 |
M14 |
Apoptosis assay |
|
24 to 48 hrs |
|
Induction of apoptosis human M14 cells assessed as caspase activity after 24 to 48 hrs using DEVD peptide as substrate by ApoTox-Glo triplex assay |
24471466 |
U937 |
Function assay |
1 uM |
24 hrs |
|
Inhibition of HDAC6 in human U937 cells assessed as increase of intracellular acetylated alpha-tubulin level at 1 uM after 24 hrs by Western blot analysis |
24694055 |
U937 |
Function assay |
1 uM |
24 hrs |
|
Inhibition of HDAC1 in human U937 cells assessed as increase of intracellular acetylated histone H3 level at 1 uM after 24 hrs by Western blot analysis |
24694055 |
U937 |
Function assay |
1 uM |
24 hrs |
|
Inhibition of HDAC2 in human U937 cells assessed as increase of intracellular acetylated histone H3 level at 1 uM after 24 hrs by Western blot analysis |
24694055 |
U937 |
Function assay |
1 uM |
24 hrs |
|
Inhibition of HDAC3 in human U937 cells assessed as increase of intracellular acetylated histone H4 level at 1 uM after 24 hrs by Western blot analysis |
24694055 |
MV4-11 |
Function assay |
10 to 1000 nM |
6 hrs |
|
Inhibition of HDAC6 in human MV4-11 cells assessed as upregulation of alpha tubulin acetylation at 10 to 1000 nM after 6 hrs by Western blot analysis |
26443078 |
HCT116 |
Function assay |
10 to 1000 nM |
6 hrs |
|
Inhibition of HDAC6 in human HCT116 cells assessed as upregulation of alpha tubulin acetylation at 10 to 1000 nM after 6 hrs by Western blot analysis |
26443078 |
HCT116 |
Function assay |
10 to 1000 nM |
6 hrs |
|
Inhibition of HDAC1/2/3 in human HCT116 cells assessed as upregulation of histone H3 acetylation at 10 to 1000 nM after 6 hrs by Western blot analysis |
26443078 |
MV4-11 |
Function assay |
10 to 1000 nM |
6 hrs |
|
Inhibition of HDAC1/2/3 in human MV4-11 cells assessed as upregulation of histone H3 acetylation at 10 to 1000 nM after 6 hrs by Western blot analysis |
26443078 |
Raji |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human Raji cells assessed as growth inhibition after 24 hrs by MTT assay |
27186676 |
Ramos |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human Ramos cells assessed as growth inhibition after 24 hrs by MTT assay |
27186676 |
U266 |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human U266 cells assessed as growth inhibition after 24 hrs by MTT assay |
27186676 |
RPMI8226 |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human RPMI8226 cells assessed as growth inhibition after 24 hrs by MTT assay |
27186676 |
HBL1 |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human HBL1 cells assessed as growth inhibition after 24 hrs by MTT assay |
27186676 |
MM1S |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human MM1S cells assessed as growth inhibition after 24 hrs by MTT assay |
27186676 |
OCI-LY1 |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human OCI-LY1 cells assessed as growth inhibition after 24 hrs by MTT assay |
27186676 |
SUDHL4 |
Cytotoxicity assay |
|
24 hrs |
|
Cytotoxicity against human SUDHL4 cells assessed as growth inhibition after 24 hrs by MTT assay |
27186676 |
MV4-11 |
Function assay |
50 nM |
24 hrs |
|
Induction of HDAC6 degradation in human MV4-11 cells at 50 nM after 24 hrs by Western blot method |
29589441 |
MV4-11 |
Function assay |
50 nM |
2 to 24 hrs |
|
Inhibition of HDAC6 in human MV4-11 cells assessed as induction of alpha-tubulin hyperacetylation at 50 nM after 2 to 24 hrs by Western blot method |
29589441 |
MV4-11 |
Cell cycle arrest assay |
30 to 50 nM |
24 hrs |
|
Cell cycle arrest in human MV4-11 cells assessed as accumulation at sub-G1 phase at 30 to 50 nM after 24 hrs by propidium iodide staining-based flow cytometric method |
29589441 |
MV4-11 |
Apoptosis assay |
30 nM |
24 to 48 hrs |
|
Induction of apoptosis in human MV4-11 cells at 30 nM after 24 to 48 hrs by Annexin V-PI staining based flow cytometry |
29738953 |