Home NF-κB NF-κB inhibitor Withaferin A (WFA) For research use only.

Withaferin A (WFA)

Synonyms: WA

Withaferin A (WFA) potently inhibits NF-κB activation by preventing the tumor necrosis factor-induced activation of IκB kinase β via a thioalkylation-sensitive redox mechanism. Withaferin A binds to the intermediate filament (IF) protein, vimentin with antitumor and antiangiogenesis activity. Withaferin A is a steroidal lactone isolated from Withania somnifera.

Withaferin A (WFA) Chemical Structure

Withaferin A (WFA) Chemical Structure

CAS No. 5119-48-2

Purity & Quality Control

Withaferin A (WFA) Related Products

Signaling Pathway

NF-κB Signaling Pathways

NF-κB Signaling Pathway

Biological Activity

Description Withaferin A (WFA) potently inhibits NF-κB activation by preventing the tumor necrosis factor-induced activation of IκB kinase β via a thioalkylation-sensitive redox mechanism. Withaferin A binds to the intermediate filament (IF) protein, vimentin with antitumor and antiangiogenesis activity. Withaferin A is a steroidal lactone isolated from Withania somnifera.
Targets
NF-κB [1] vimentin [2]
In vitro
In vitro

Withaferin A potently inhibits NFκB activation by preventing the tumor necrosis factor-induced activation of IκB kinase β via a thioalkylation-sensitive redox mechanism.[1] Withaferin A binds to the intermediate filament (IF) protein, vimentin, by covalently modifying its cysteine residue, which is present in the highly conserved α-helical coiled coil 2B domain. Withaferin A induces vimentin filaments to aggregate in vitro.[2]
Cell Research Cell lines HUVECs, BAECs,MCF-7 breast cancer cell line, MFT-16 cells
Concentrations 5 μM, 10 μM
Incubation Time 1 h, 2 h, 24 h
Method

To assess the apoptosis activity of WFA and 12-D WS, embryonic fibroblast vimentindeficient cell lines (Vim−/−; MFT-16) and wild-type (Vim+/+; MFT-6) cells are treated with 5 μM WFA, 5 μM 12-D WS, or an equivalent amount of vehicle (DMSO) for 24 hr in 37℃-5% CO2 conditions in complete medium. Apoptotic cells are measured with the Vybrant Apoptosis Assay Kit according to the manufacturer's instructions.
In Vivo
In vivo

Withagerin A clearly displays anti-inflammatory characteristics in an acute inflammatory mouse model.[1] Withagerin A-induced inhibition of capillary growth in a mouse model of corneal neovascularization is compromised in vimentin-deficient mice.[2]
Animal Research Animal Models 8-week-old C57BL/6J mice
Dosages 0.5 mg/mouse
Administration IP
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01199250 Not yet recruiting
Lynch Syndrome|Recurrent Uterine Corpus Carcinoma|Stage I Uterine Corpus Cancer|Stage II Uterine Corpus Cancer|Stage III Uterine Corpus Cancer|Stage IV Uterine Corpus Cancer
Gynecologic Oncology Group|National Cancer Institute (NCI)|GOG Foundation
 January 2100 --
NCT04432610 Withdrawn
Nebivolol Adverse Reaction
Clinical Hospital Center Zemun
 January 2030 Phase 4
NCT06376812 Not yet recruiting
Elevated Blood Pressure|Perimenopause
The University of Texas Medical Branch Galveston
 May 2026 Not Applicable

Chemical Information & Solubility

Molecular Weight 470.60 Formula

C28H38O6
CAS No. 5119-48-2 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 94 mg/mL ( (199.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble


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In vivo
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