Name: QNZ (EVP4593)
Brand: Selleck Chemicals
SKU: S4902
Availability: InStock
Rating: 5 (157 reviews)

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Quality Control

Batch: Purity: 99.94%

99.94

Related Targets	HDAC Antioxidant ROS IκB/IKK Nrf2 AP-1 MALT NOD
Other NF-κB Inhibitors	Omaveloxolone (RTA-408) DCZ0415 BAY 11-7082 (BAY 11-7821) JSH-23 SC75741 Caffeic Acid Phenethyl Ester DHA (Dihydroartemisinin) Andrographolide Evodiamine Withaferin A (WFA)

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
Jurkat	Kinase assay	~10 μM		causes NF-κB Inhibition with EC50 of 9 nM	21700213
Jurkat	Function assay	3 μM		causes SOC Inhibition	21700213
neurons	Function assay	300 nM		inhibit store-operated Ca2+ entry	21700213
SK-N-SH	Function assay	300 nM		inhibits TRPC1-Supported SOC Ca2+ currents	21700213
neurons	Function assay	300 nM		protect YAC128 MSN from glutamate toxicity	21700213
GABA MS-like neurons	Function assay	100 nM		rescues abnormal SOC-mediated calcium entry	27080129
GABA MS-like neurons	Function assay	1000 nM		normalizes the number of lysosomes/autophagosomes	27080129
GABA MS-like neurons	Function assay	100 nM		rescues aging neurons from cell death	27080129
HepG2	Function assay			HARVARD: Inhibition of liver stage Plasmodium berghei infection in HepG2 cells, IC50 = 0.012 μM.	22586124
HepG2	Function assay	0.1 to 10 uM	1 hr	Inhibition of TNFalpha-induced NFkappaB (unknown origin)-dependent transcriptional activity expressed in human HepG2 cells at 0.1 to 10 uM incubated for 1 hr prior to TNFalpha induction measured after 6 hrs by dual-luciferase reporter gene assay	23219854
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 7 mg/mL (19.63 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	0.5% hydroxyethyl cellulose Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	10.000mg/ml (28.06mM)	Taking the 1 mL working solution as an example, take 10 mg of this product, add it to 1 ml of 0.5% hydroxyethyl cellulose clear solution, and mix evenly to form a uniform suspension. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.550mg/ml (9.96mM)	Taking the 1 mL working solution as an example, add 50 μL of 71 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	356.42	Formula	C₂₂H₂₀N₄O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	545380-34-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1=CC=C(C=C1)OC2=CC=C(C=C2)CCNC3=NC=NC4=C3C=C(C=C4)N

Mechanism of Action

Targets/IC₅₀/Ki	TNF-α (Jurkat T cells) 7 nM NF-κB (Jurkat T cells) 11 nM
In vitro	QNZ (EVP4593) inhibits TNF-a production from murine splenocytes stimulated with LPS with IC50 of 7 nM. This compound (300 nM) entails a significant decrease of amplitude of store-operated currents (approximately by 60%), induced by application of 1 μM thapsigargin in Htt138Q cells, thus prevents abnormal store-operated calcium entry. It is able to inhibit the activity of channels containing TRPC1 as one of the subunits, but has no effect on homooligomer channels composed exclusively of TRPC1. At 40 nM, it completely abolishes the enhancement of neurite number and length evoked by laminin treatment of the schwann cells. QNZ reduces the neurite length by 61.36% of the schwann cells. It significantly inhibits laminin-induced neurite outgrowth. It also greatly diminishes the neurite elongation after 72 hours culture implying that both initial sprouting and longer term growth and extension seen in response to schwann cells seeded on laminin is mediated by NF-κB. At 10 nM, it abolishes LPS-induced up-regulation of CSE expression in rat neutrophil. At 100 nM, it blocks the induction effects of GRO/KC on K currents in IB4-negative neurons.
Kinase Assay	NF-κB assay
Kinase Assay	Human Jurkat T cells are cultured at 37℃ in a 5% CO₂ atmosphere in RPMI1640 containing 10% FCS. The cells are plated in 6-well plates (2×10⁶/well) and transiently transfected using the SuperFect Transfection Reagent with 1 μg of pNFκB-Luc. After transfection, the cells are cultured at 37℃ overnight. They are then collected, resuspended in fresh medium, and plated in 96-well plates (2×10⁵/well). QNZ (EVP4593) is dissolved in DMSO and added at the appropriate concentrations to the 96-well plates containing the cells, and the plates are then incubated at 37℃ for 1 hour. For induction of transcription, 10 ng/mL of PMA and 100 μg/mL of PHA are added to each well, and the cells are incubated for an additional 6 hours at 37℃. The culture media are removed, and cell lysis buffer containing luciferase substrate is added to each well. Each portion is transferred to a black 96-well plate, and then luminescence is immediately measured with a Packard Topcount. The 50% inhibitory concentration (IC₅₀) values are calculated by a nonlinear regression method.
In vivo	QNZ (EVP4593) (1 mg/kg, i.p.) dose-dependently inhibits carrageenin-induced paw edema in rats.
References	[1] https://pubmed.ncbi.nlm.nih.gov/12517433/ [2] http://link.springer.com/article/10.1134/S199074781201014X [3] https://pubmed.ncbi.nlm.nih.gov/18502047/ [4] https://pubmed.ncbi.nlm.nih.gov/19120693/ [5] https://pubmed.ncbi.nlm.nih.gov/19476648/

Applications

Methods	Biomarkers	Images	PMID
Western blot	NF-κB p65 / OPN / Tissue factor / Thrombin VEGF / TNF-α / IL-1β / IL-6 / MMP-2 / MMP-9 / NF-κB p65(Ser536)		29061995
Growth inhibition assay	Cell viability		28693192

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Handling Instructions

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QNZ (EVP4593) NF-κB inhibitor

Chemical Structure

Molecular Weight: 356.42