Home Immunology & Inflammation Nrf2 activator - HDAC inhibitor - NF-κB inhibitor - ROS inhibitor - Heme Oxygenase activator Sulforaphane For research use only.

Sulforaphane

Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.,

Sulforaphane Chemical Structure

Sulforaphane Chemical Structure

CAS No. 4478-93-7

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Sulforaphane Related Products

Biological Activity

Description Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.,
Targets
Nrf2 [1] HDAC [1] NF-κB [1] ROS [1] HO-1 [1]
In vitro
In vitro

Sulforaphane, reactive oxygen species (ROS) inhibitor, decrease the autophagy induced by CC90003, while cell death strengthens, confirming that CC90003 induces autophagy by activating ROS/p53.[3]
Cell Research Cell lines Human CRC cell HCT-116 and SW620
Concentrations 20 μM
Incubation Time 24 h
Method

Cells are cultured in a 96-well microplate at a density of 5×103 cells/well for 24 h. Then, the cells are divided into several groups and treated with different conditions. Cell viability is assessed with CCK-8 after treating drugs. The absorbance value at 450 nm is read with a 96-well plate reader. Nuclear staining with Hoechst33342 is used to analyze the apoptosis. For colony formation assays, an equal number of cells after different treatments are planted in six-well plates.Colonies are visualized by crystal violet staining 14 days after plating.
Experimental Result Images Methods Biomarkers Images PMID
Western blot β-catenin / Cyclin D1 EGFR / p-EGFR / STAT3 / p-STAT3 / AKT / p-AKT 20388854
Growth inhibition assay Cell viability 30226534
Immunofluorescence HER2 Gli1 / Gli2 30226534
In Vivo
In vivo

Natural isothiocyanate and potent NRF2 activator sulforaphane reverses diet-induced obesity through a predominantly, but not exclusively, NRF2-dependent mechanism that requires a functional leptin receptor signaling and hyperleptinemia.[4]
Animal Research Animal Models diet-induced obese mice
Dosages 25 μl
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03934905 Recruiting
Anthracycline Related Cardiotoxicity in Breast Cancer
Texas Tech University Health Sciences Center
 June 1 2022 Phase 1|Phase 2
NCT05073523 Completed
Healthy
Chalmers University of Technology
 September 27 2021 Not Applicable
NCT05367024 Enrolling by invitation
Pre-diabetes
Quadram Institute Bioscience|Norfolk and Norwich University Hospitals NHS Foundation Trust
 September 27 2021 Not Applicable

Chemical Information & Solubility

Molecular Weight 177.29 Formula

C6H11NOS2
CAS No. 4478-93-7 SDF --
Density 1.17 g/mL
Storage (From the date of receipt) 2 years -20°C(in the dark) liquid

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