Home Immunology & Inflammation Nrf2 inhibitor - Ferroptosis modulator ML385 For research use only.

ML385

ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.

ML385 Chemical Structure

ML385 Chemical Structure

CAS No. 846557-71-9

Purity & Quality Control

Products often used together with ML385

Brusatol

ML385 and Brusatol use significantly reduces ABCC5 expression and downregulates NRF2 expression in Sk-Hep-1 cells.

Huang W, et al. Neoplasia. 2021 Dec;23(12):1227-1239.

RSL3

ML385 and RSL3, when used together, synergistically and significantly inhibit cell proliferation in MV4;11/Kasumi-1/KG1α cells.

Liu X, et al. Exp Hematol Oncol. 2023 May 17;12(1):47.

ML385 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MG-63 cells Function assay 2 μM 2 h and 4 h ML385 (2 µmol/L) could significantly increase cellular ROS level by ~2–4-fold in MG-63 cells after co-incubation (with graphene oxide) 29844673
A549 Function assay 0, 5 and 10 μM 24 and 48 h A549 cells treated with ML385 demonstrated a dose-dependent reduction in NRF2 protein levels and its target genes 27552339
Click to View More Cell Line Experimental Data

Biological Activity

Description ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.
Targets
Ferroptosis [3] Nrf2 [1]
1.9 μM
In vitro
In vitro

ML385 shows anti-tumor activity in NSCLC (subcutaneous and orthotopic NSCLC models) both as a single agent and in combination. The PK profile tested in CD-1 mice shows that ML385 has a half-life (t1/2 = 2.82 h) after IP injection (30 mg/kg)[1]. ML385 could alleviate the pancreatic injury[2].
Cell Research Cell lines A549 cells
Concentrations 5 μM
Incubation Time 24, 48 and 72 h
Method

--
Experimental Result Images Methods Biomarkers Images PMID
Western blot Nrf2 31456942
Immunofluorescence Nrf2 31089407

Chemical Information & Solubility

Molecular Weight 511.59 Formula

C29H25N3O4S
CAS No. 846557-71-9 SDF --
Smiles CC1=CC=CC=C1C(=O)N2CCC3=C2C=CC(=C3)C4=C(SC(=N4)NC(=O)CC5=CC6=C(C=C5)OCO6)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 20 mg/mL ( (39.09 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy ML385 | ML385 supplier | purchase ML385 | ML385 cost | ML385 manufacturer | order ML385 | ML385 distributor