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ML385

Name: ML385
Brand: Selleck Chemicals
SKU: S8790
Rating: 5 (119 reviews)

Catalog No.S8790 Purity:99.92%

ML385 is a novel and specific NRF2 inhibitor with an IC50 of 1.9 μM. It inhibits the downstream target gene expression of NRF2. NRF2 regulates the activity of several ferroptosis and lipid peroxidation-related proteins.

ML385 Chemical Structure

CAS No. 846557-71-9

Purity & Quality Control

Batch: Purity: 99.92%

99.92

Products often used together with ML385

Brusatol

ML385 and Brusatol use significantly reduces ABCC5 expression and downregulates NRF2 expression in Sk-Hep-1 cells.

Huang W, et al. Neoplasia. 2021 Dec;23(12):1227-1239.

RSL3

ML385 and RSL3, when used together, synergistically and significantly inhibit cell proliferation in MV4;11/Kasumi-1/KG1α cells.

Liu X, et al. Exp Hematol Oncol. 2023 May 17;12(1):47.

ML385 Related Products

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Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MG-63 cells	Function assay	2 μM	2 h and 4 h	ML385 (2 µmol/L) could significantly increase cellular ROS level by ~2–4-fold in MG-63 cells after co-incubation (with graphene oxide)	29844673
A549	Function assay	0, 5 and 10 μM	24 and 48 h	A549 cells treated with ML385 demonstrated a dose-dependent reduction in NRF2 protein levels and its target genes	27552339
Click to View More Cell Line Experimental Data

Biological Activity

Description

Targets

Ferroptosis ^[3]	Nrf2 ^[1]
	1.9 μM

In vitro
In vitro	ML385 shows anti-tumor activity in NSCLC (subcutaneous and orthotopic NSCLC models) both as a single agent and in combination. The PK profile tested in CD-1 mice shows that ML385 has a half-life (t_1/2 = 2.82 h) after IP injection (30 mg/kg)^[1]. ML385 could alleviate the pancreatic injury^[2].
Cell Research	Cell lines	A549 cells
	Concentrations	5 μM
	Incubation Time	24, 48 and 72 h
	Method	--
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	Nrf2		31456942
	Immunofluorescence	Nrf2		31089407

References

Chemical Information & Solubility

Molecular Weight	511.59	Formula	C₂₉H₂₅N₃O₄S
CAS No.	846557-71-9	SDF	--
Smiles	CC1=CC=CC=C1C(=O)N2CCC3=C2C=CC(=C3)C4=C(SC(=N4)NC(=O)CC5=CC6=C(C=C5)OCO6)C
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 20 mg/mL ( (39.09 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	6%DMSO 1 40%PEG300 2 5%Tween80 3 49%ddH2O	1.5mg/ml (2.93mM)	Taking the 1 mL working solution as an example, add 60 μL 25 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 490 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	6%DMSO 1 40%PEG300 2 5%Tween80 3 49%ddH2O	1.5mg/ml (2.93mM)	Taking the 1 mL working solution as an example, add 60 μL 25 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 490 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	10% DMSO 1 40% PEG 300 2 5%Tween80 3 45%ddH2O	3.0mg/ml (5.86mM)	Taking the 1 mL working solution as an example, add 100 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 450 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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