Home Autophagy Autophagy activator Spermidine For research use only.

Spermidine

Spermidine is a natural polyamine that stimulates cytoprotective macroautophagy/autophagy.

Spermidine Chemical Structure

Spermidine Chemical Structure

CAS No. 124-20-9

Purity & Quality Control

Spermidine Related Products

Signaling Pathway

Autophagy Signaling Pathways

Autophagy Signaling Pathway

Biological Activity

Description Spermidine is a natural polyamine that stimulates cytoprotective macroautophagy/autophagy.
In vitro
In vitro

Spermidine and spermine significantly increases the viability and proliferation of SKOV-3 cells in a time-dependent manner, and decreases the apoptotic effect of Difluoromethylornithine (DFMO) in SKOV-3 cells. [1]
Cell Research Cell lines SKOV-3 cells
Concentrations 50 μM
Incubation Time 1–5 days
Method

SKOV-3 cells are incubated with 50 μM spermidine or spermine for 1–5 days, and then cell viability and proliferation are determined using the PrestoBlue or CellTiter-Glo assays, respectively.
In Vivo
In vivo

Spermidine, a selective activator of TC45, rescues exacerbated phenotype of Hematopoietic stem and progenitor cells (HSPCs) in IFN‐γ‐treated Hemgn-/- mice.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06017219 Active not recruiting
Healthy Subjects
Chrysea Labs Lda
 May 5 2023 Not Applicable
NCT05421546 Completed
COVID-19|Vaccine Reaction
University of Oxford
 August 1 2022 Not Applicable
NCT04138134 Active not recruiting
Venous Disease
Neuromed IRCCS|Principal investigator: Giacomo Frati|Co-investigator: Francesco Pompeo
 February 1 2022 --
NCT04823806 Active not recruiting
Depression|Healthy
University Hospital Bonn
 December 1 2020 Not Applicable
NCT02855918 Completed
Major Depression
University Hospital Montpellier|Institut National de la Santé Et de la Recherche Médicale France|Sys2Diag Mixt laboratory CNRS/Alcediag Montpellier
 September 23 2016 Not Applicable

Chemical Information & Solubility

Molecular Weight 145.25 Formula

C7H19N3
CAS No. 124-20-9 SDF --
Density 1g/ml
Storage (From the date of receipt) 2 years -20°C liquid

In vitro
Batch:

Water : 100 mg/mL

DMSO : 20 mg/mL ( (137.69 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Spermidine | Spermidine supplier | purchase Spermidine | Spermidine cost | Spermidine manufacturer | order Spermidine | Spermidine distributor