Home Immunology & Inflammation ROS inhibitor Pyrroloquinoline quinone For research use only.

Pyrroloquinoline quinone

Synonyms: PQQ, Methoxatin

Pyrroloquinoline quinone (PQQ, Methoxatin) is a natural anti-oxidant with anti-oxidative and anti-aging effects. Pyrroloquinoline quinone prevents estrogen deficiency-induced osteoporosis.Pyrroloquinoline quinone can alleviate allergic airway inflammation in mice by improving the immune microenvironment and regulating the JAK-STAT signaling pathway. This product has poor solubility, animal experiments are available, cell experiments please choose carefully!

Pyrroloquinoline quinone Chemical Structure

Pyrroloquinoline quinone Chemical Structure

CAS No. 72909-34-3

Purity & Quality Control

Batch: S357801 DMSO]Insoluble]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.88%
99.88

Pyrroloquinoline quinone Related Products

Biological Activity

Description Pyrroloquinoline quinone (PQQ, Methoxatin) is a natural anti-oxidant with anti-oxidative and anti-aging effects. Pyrroloquinoline quinone prevents estrogen deficiency-induced osteoporosis.Pyrroloquinoline quinone can alleviate allergic airway inflammation in mice by improving the immune microenvironment and regulating the JAK-STAT signaling pathway. This product has poor solubility, animal experiments are available, cell experiments please choose carefully!
In vitro
In vitro

The activation of AMPK by PQQ is required for the regulation of mitochondrial biogenesis in rotenone-injured SH-SY5Y cells, the upregulation of PGC-1α induced by PQQ is reduced by inhibition of AMPK.[2]
Cell Research Cell lines SH-SY5Y human neuroblastoma cells
Concentrations 100 μM
Incubation Time 30 min
Method

SH-SY5Y human neuroblastoma cells were cultured in DMEM supplemented with 10% FBS at 37 °C in a humidified atmosphere of 95% air and 5% CO2. The day before treatment, cells were plated in a 10-cm culture dish at a density of 1 × 106 cells/mL. The cells were divided into four groups: the control group (no rotenone or PQQ), PQQ group (100 μM PQQ), Rotenone group (100 μM rotenone), and PQQ + Rotenone group (100 μM PQQ + 100 μM rotenone). Thirty minutes after treatment, the cells were washed once with cold PBS and scraped in urea lysis buffer (9 M sequanal-grade urea, 20 mM HEPES [pH 8.0], 1 mM β-glyceropho_x005fsphate, 1 mM sodium vanadate, 2.5 mM sodium pyrophosphate). The cells were sonicated in lysis buffer and centrifuged for 15 min at 4 °C and 20,000 × g. Supernatants were collected and reduced with 4.5 mM DTT for 30 min at 55 °C. Reduced lysates were alkylated with iodoacetamide (0.019 g/mL of H2O) for 15 min in the dark. The samples were diluted 1:4 with 20 mM HEPES [pH 8.0] and digested with 10 g/mL trypsin-tosylphenylalanyl chloromethyl ketone in 1 mM HCl overnight. Digested peptide lysates were acidified with 1% TFA, and the peptides were desalted over 360- mg SEP PAK Classic C18 columns. Peptides were eluted with 40% acetonitrile in 0.1% TFA, dried under a vacuum, and stored at −80 °C. Differentially expressed phosphoproteins were identified and analyzed.
In Vivo
In vivo

PQQ dose-dependently alleviates the locomotor deficits and nigral dopaminergic neuron loss in PD mice, significantly diminishes the reduction of mitochondria number and their pathological change in the midbrain, also dose-dependently blocks rotenone-caused reduction in the expression of PGC-1α and TFAM, two key activators of mitochondrial gene transcription, in the midbrain.[2]
Animal Research Animal Models Adult ICR mice of PD model
Dosages 0.8, 4, and 20 mg/kg/d
Administration i.p.

Chemical Information & Solubility

Molecular Weight 330.21 Formula

C14H6N2O8
CAS No. 72909-34-3 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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