Pyrroloquinoline quinone

Catalog No.S3578 Batch:S357801

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Technical Data

Formula

C14H6N2O8

Molecular Weight 330.21 CAS No. 72909-34-3
Solubility (25°C)* In vitro DMSO Insoluble
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Pyrroloquinoline quinone (PQQ, Methoxatin) is a natural anti-oxidant with anti-oxidative and anti-aging effects. Pyrroloquinoline quinone prevents estrogen deficiency-induced osteoporosis.Pyrroloquinoline quinone can alleviate allergic airway inflammation in mice by improving the immune microenvironment and regulating the JAK-STAT signaling pathway. This product has poor solubility, animal experiments are available, cell experiments please choose carefully!
In vitro

The activation of AMPK by PQQ is required for the regulation of mitochondrial biogenesis in rotenone-injured SH-SY5Y cells, the upregulation of PGC-1α induced by PQQ is reduced by inhibition of AMPK.[2]

In vivo

PQQ dose-dependently alleviates the locomotor deficits and nigral dopaminergic neuron loss in PD mice, significantly diminishes the reduction of mitochondria number and their pathological change in the midbrain, also dose-dependently blocks rotenone-caused reduction in the expression of PGC-1α and TFAM, two key activators of mitochondrial gene transcription, in the midbrain.[2]

Protocol (from reference)

Cell Assay:

[2]

  • Cell lines

    SH-SY5Y human neuroblastoma cells

  • Concentrations

    100 μM

  • Incubation Time

    30 min

  • Method

    SH-SY5Y human neuroblastoma cells were cultured in DMEM supplemented with 10% FBS at 37 °C in a humidified atmosphere of 95% air and 5% CO2. The day before treatment, cells were plated in a 10-cm culture dish at a density of 1 × 106 cells/mL. The cells were divided into four groups: the control group (no rotenone or PQQ), PQQ group (100 μM PQQ), Rotenone group (100 μM rotenone), and PQQ + Rotenone group (100 μM PQQ + 100 μM rotenone). Thirty minutes after treatment, the cells were washed once with cold PBS and scraped in urea lysis buffer (9 M sequanal-grade urea, 20 mM HEPES [pH 8.0], 1 mM β-glyceropho_x005fsphate, 1 mM sodium vanadate, 2.5 mM sodium pyrophosphate). The cells were sonicated in lysis buffer and centrifuged for 15 min at 4 °C and 20,000 × g. Supernatants were collected and reduced with 4.5 mM DTT for 30 min at 55 °C. Reduced lysates were alkylated with iodoacetamide (0.019 g/mL of H2O) for 15 min in the dark. The samples were diluted 1:4 with 20 mM HEPES [pH 8.0] and digested with 10 g/mL trypsin-tosylphenylalanyl chloromethyl ketone in 1 mM HCl overnight. Digested peptide lysates were acidified with 1% TFA, and the peptides were desalted over 360- mg SEP PAK Classic C18 columns. Peptides were eluted with 40% acetonitrile in 0.1% TFA, dried under a vacuum, and stored at −80 °C. Differentially expressed phosphoproteins were identified and analyzed.

Animal Study:

[2]

  • Animal Models

    Adult ICR mice of PD model

  • Dosages

    0.8, 4, and 20 mg/kg/d

  • Administration

    i.p.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.