Home Immunology & Inflammation ROS inhibitor MitoQ (Mitoquinone) mesylate For research use only.

MitoQ (Mitoquinone) mesylate

Synonyms: MitoQ10 mesylate, Mitoubiquinone mesylate

MitoQ (Mitoquinone) mesylate is a TPP-based, mitochondria-targeted antioxidant that blocks H2O2-induced intracellular ROS responses and protects against oxidative damage. This product is a waxy solid.

MitoQ (Mitoquinone) mesylate Chemical Structure

MitoQ (Mitoquinone) mesylate Chemical Structure

CAS No. 845959-50-4

Purity & Quality Control

Products often used together with MitoQ (Mitoquinone) mesylate

Visomitin (SKQ1)

Mitoquinone (MitoQ10) mesylate and Visomitin (SKQ1) specifically target the mitochondrial matrix and are highly effective at limiting reactive oxygen species (ROS) levels.

Moustapha A, et al. Cell Death Discov. 2015 Oct 26;1:15017.

Trolox

MitoQ10 completely inhibits mitochondrial lipid peroxidation, whereas Trolox only partially inhibits mitochondrial lipid peroxidation in pretreated hepatocytes.

Gonzalvez F, et al. PLoS One. 2010 Feb 22;5(2):e9342.

MitoQ (Mitoquinone) mesylate Related Products

Biological Activity

Description MitoQ (Mitoquinone) mesylate is a TPP-based, mitochondria-targeted antioxidant that blocks H2O2-induced intracellular ROS responses and protects against oxidative damage. This product is a waxy solid.
Targets
mitochondria [1] ROS [1]
In vitro
In vitro

Mitoquinone blocks H2O2-induced intracellular ROS responses inmurine pancreatic acinar cells. Mitoquinone does not reduce mitochondrial depolarisation induced by either cholecystokinin (CCK) or bile acid TLCS. Mitoquinone increases basal and CCK-induced cell death in a plate-reader assay.[1]
Cell Research Cell lines Pancreatic Acinar Cells
Concentrations 1 μM, 10 μM
Incubation Time 200 s, 400 s, 600 s, 800 s, 1000 s
Method

The cells are loaded with 40 nM TMRM for 30 minutes prior incubation with either 1 μM or 10 μM of MitoQ or dTPP. Cholecystokinin-8 (CCK-8, 10 nM) or bile acid taurolithocholic acid 3-sulphate (TLCS, 500 μM) is used to induce ΔΨm depolarisation. At the end of the perfusion, the protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 10 μM) is added to induce complete depolarisation of ΔΨm. The fluorescence of TMRM is excited at 543 nm and the emission is collected at 560–650 nm.
In Vivo
In vivo

In a TLCS-induced AP model Mitoquinone treatment is not protective. In AP induced by caerulein hyperstimulation (CER-AP), Mitoquinone exerts mixed effects. Thus, partial amelioration of histopathology scores is observed but without reduction of the biochemical markers pancreatic trypsin or serumamylase. Lungmyeloperoxidase and interleukin-6 are concurrently increased by Mitoquinone in CER-AP. Mitoquinone causes biphasic effects on ROS production in isolated polymorphonuclear leukocytes, inhibiting an acute increase but elevating later levels.[1]
Animal Research Animal Models C57BL/6J mice
Dosages 10 mg/kg, 25 mg/kg
Administration IP
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05539625 Not yet recruiting
Ulcerative Colitis
University of Edinburgh|The Jon Moulton Charity Trust|MitoQ
 September 2022 Phase 2
NCT04109820 Recruiting
Sickle Cell Disease
University of Pittsburgh
 March 1 2020 Not Applicable
NCT04098510 Unknown status
Healthy
University of Copenhagen
 September 8 2019 Not Applicable
NCT01167088 Terminated
Non-alcoholic Fatty Liver Disease
Antipodean Pharmaceuticals Inc.
 November 2010 Phase 2
NCT00433108 Completed
Chronic Hepatitis C
Antipodean Pharmaceuticals Inc.
 March 2007 Phase 2

Chemical Information & Solubility

Molecular Weight 678.81 Formula

C38H47O7PS
CAS No. 845959-50-4 SDF --
Smiles CC1=C(C(=O)C(=C(C1=O)OC)OC)CCCCCCCCCC[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4.CS(=O)(=O)[O-]
Storage (From the date of receipt) 2 years -20°C liquid

In vitro
Batch:

DMSO : 50 mg/mL ( (73.65 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 10 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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