Home Endocrinology & Hormones Estrogen/progestogen Receptor agonist Progesterone For research use only.

Progesterone

Synonyms: NSC 64377, Pregn-4-ene-3,20-dione,NSC 9704

Progesterone is an endogenous steroid hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. A potent agonist of the nuclear progesterone receptor (nPR) with Kd of 1 nM; An agonist of the membrane progesterone receptors(mPRs); An antagonist of the σ1 receptor.

Progesterone Chemical Structure

Progesterone Chemical Structure

CAS No. 57-83-0

Purity & Quality Control

Progesterone Related Products

Signaling Pathway

Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human T47D cells Function assay 20 h Activation of progesterone receptor in human T47D cells after 20 hrs by PRE-tagged luciferase reporter gene assay, EC50=0.1 nM 19863083
CV-1 cells Function assay Effective concentration for half-maximal activation of human progesterone receptor expressed in CV-1 cells, EC50=1.5 nM 9667968
HEK293 cells Function assay Agonist activity at human PRGR expressed in HEK293 cells by luciferase reporter gene assay, EC50=2 nM 25305688
SF-12 cells Function assay Displacement of DHT from human androgen receptor expressed in baculovirus SF-12 cells, Ki=9.5 nM 12954062
CHO cells Function assay Agonist activity at human TGR5 expressed in CHO cells by luciferase assay, EC50=2.77 μM 18307294
human K562/R7 cells Function assay 1 μM 72 h Potentiation of doxorubicin-induced cytotoxicity against doxorubicin-resistant human K562/R7 cells assessed as doxorubicin IC50 at 1 uM after 72 hrs by MTT assay, IC50=10.6 μM 25634041
A2780 cells Function assay Inhibition of P-gp in human adriamycin-resistant A2780 cells by Hoechst 33342 assay, IC50=47.863 μM 18678495
HeLa cells Cytotoxicity assay Cytotoxicity against human HeLa cells assessed as inhibition of DNA replication by imaging analysis 18066055
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Biological Activity

Description Progesterone is an endogenous steroid hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. A potent agonist of the nuclear progesterone receptor (nPR) with Kd of 1 nM; An agonist of the membrane progesterone receptors(mPRs); An antagonist of the σ1 receptor.
Targets
progestogen Receptor [1]
In vitro
In vitro

Progesterone has biphasic effects on proliferation of breast cancer cells; it stimulates growth in the first cell cycle, then arrests cells at G1/S of the second cycle accompanied by up-regulation of the cyclin-dependent kinase inhibitor, p21. Progesterone-mediated transcription is further prevented by overexpression of E1A, suggesting that CBP/p300 is required. [1] Progesterone drives a series of events where luminal cells probably provide Wnt4 and RANKL signals to basal cells which in turn respond by upregulating their cognate receptors, transcriptional targets and cell cycle markers. [2] Progesterone treatment increases the sensitivity of cortical synaptoneurosomes to GABA (i.e., decreased the EC50) and increases the maximal efficacy with which GABA stimulated Cl- transport (i.e., increased the Emax). [3]
In Vivo
In vivo

Progesterone blocks the beneficial effect of estrogen on Abeta accumulation but not on behavioral performance in female 3xTg-AD mice. Progesterone significantly reduces tau hyperphosphorylation when administered both alone and in combination with estrogen. [4] Progesterone-treated rats are less impaired on a Morris water maze spatial navigation task than rats treated with the oil vehicle. Progesterone-treated rats also show less neuronal degeneration 21 days after injury in the medial dorsal thalamic nucleus, a structure that has reciprocal connections with the contused area. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06089395 Not yet recruiting
Poor Ovarian Response
Shi Yun|Capital Medical University|Dongzhimen Hospital Beijing
 June 1 2024 Early Phase 1
NCT06359457 Not yet recruiting
Primary Dysmenorrhea
Cairo University
 May 2024 --
NCT06334315 Not yet recruiting
Contraception|Pharmacogenomic Drug Interaction
Yale University|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
 May 2024 Phase 4

Chemical Information & Solubility

Molecular Weight 314.46 Formula

C21H30O2
CAS No. 57-83-0 SDF Download Progesterone SDF
Smiles CC(=O)C1CCC2C1(CCC3C2CCC4=CC(=O)CCC34C)C
Storage (From the date of receipt)

In vitro
Batch:

Ethanol : 31.5 mg/mL

DMSO : 22 mg/mL ( (69.96 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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