Home Transmembrane Transporters Antineoplastic and Immunosuppressive Antibiotics modulator - Potassium Channel chemical - NLRP3 activator Nigericin sodium salt For research use only.

Nigericin sodium salt

Synonyms: NSC 292567

Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.

Nigericin sodium salt Chemical Structure

Nigericin sodium salt Chemical Structure

CAS No. 28643-80-3

Purity & Quality Control

Nigericin sodium salt Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9 Antiviral assay Antiviral activity against HIV1 RF in human H9 cells assessed as inhibition of virus-induced cytopathic effect by formazan-based conventional colorimetric technique, EC50=0.006μM. 11430019
H9 Cytotoxicity assay Cytotoxicity against human H9 cells, IC50=0.14μM. 11430019
Click to View More Cell Line Experimental Data

Biological Activity

Description Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.
Targets
Potassium channel [1] Hydrogen channel [1] Pb 2+ [1] NLRP3 [2]
In vitro
In vitro

In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H+, K+, and Pb2+ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade[1] .

Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death. [2]
Cell Research Cell lines H460 cells
Concentrations 1 µM
Incubation Time 24 h
Method

H460 cells are seeded in 8-µm pore size Transwell® Inserts in RPMI 1640 (without FBS) with or without drugs (DMSO as control) or NIG (1µM). Inserts are placed in 24-well plates with RPMI 1640 containing 10% FBS in the presence of DMSO (control) or NIG (1 µM).
In Vivo
In vivo

Nigericin is a NOD-like receptor protein 3 (NLRP3) activator.
Animal Research Animal Models Male C57BL/6 mice
Dosages 4 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 746.94 Formula

C40H67NaO11
CAS No. 28643-80-3 SDF --
Smiles CC1CCC(OC1C(C)C(=O)[O-])CC2CC(C(C3(O2)C(CC(O3)(C)C4CCC(O4)(C)C5C(CC(O5)C6C(CC(C(O6)(CO)O)C)C)C)C)C)OC.[Na+]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Ethanol : 100 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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