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Puromycin 2HCl

Synonyms: CL13900 2HCl

Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.

Puromycin 2HCl Chemical Structure

Puromycin 2HCl Chemical Structure

CAS No. 58-58-2

Purity & Quality Control

Products often used together with Puromycin 2HCl

Blasticidin S HCl

Puromycin and Blasticidin S inhibits the proliferation and cell viability of metastatic castration-resistant prostate cancer (mCRPC) cells PC3/DU145.

Oseni SO, et al. bioRxiv (2021): 2021-08.

Melatonin

Melatonin potentiates puromycin-induced apoptosis with caspase-3 and AMPK activation in HL-60 cells.

Koh W, et al. J Pineal Res. 2011 May;50(4):367-73.

Puromycin 2HCl Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
K562 Growth inhibition assay 72 h Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay, IC50=26.83 μM. 18313307
HepG2 Growth inhibition assay 72 h Growth inhibition of human HepG2 cells after 72 hrs by MTT assay, IC50=0.23 μM. 18313307
K562 Growth inhibition assay 72 h Growth inhibition of human K562 cells after 72 hrs by SRB assay, IC50=0.22 μM. 18313307
HepG2 Growth inhibition assay 72 h Growth inhibition of human HepG2 cells after 72 hrs by SRB assay, IC50=0.21 μM. 18512984
Huh7 Cytotoxicity assay NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7), CC50=6.63 μM. 18579783
HEK239 Cytotoxicity assay 72 h Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay, IC50=0.42 μM. 20558060
J774A1 Cytotoxicity assay Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay, IC50=10 μM. 22934636
KB3-1 Growth inhibition assay 72 hrs Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay, IC50 = 0.21 μM. 18313307
HepG2/Dox Growth inhibition assay 72 hrs Growth inhibition of multidrug resistant human HepG2/Dox cells after 72 hrs by SRB assay, IC50 = 20.2 μM. 18512984
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis, IC50 = 0.361 μM. 26651537
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay, IC50 = 0.3505 μM. 27060763
A549 Cytotoxicity assay Cytotoxicity against human A549 cells, IC50 = 0.6 μM. 27089214
PC3 Cytotoxicity assay Cytotoxicity against human PC3 cells, IC50 = 0.7 μM. 27089214
HEK293 Cytotoxicity assay Cytotoxicity against HEK293 cells by alamar blue assay, IC50 = 0.00035 μM. 27212070
HEK293 Growth inhibition assay 72 hrs Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay, IC50 = 0.36 μM. 28001067
HEK293 Growth inhibition assay Growth inhibition of HEK293 cells, IC50 = 0.399 μM. 28400231
SU-DHL10 Cytotoxicity assay 72 hrs Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.11 μM. 28523103
MIAPaCa2 Cytotoxicity assay 72 hrs Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.2 μM. 28523103
MDA-MB-231 Cytotoxicity assay 72 hrs Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.48 μM. 28523103
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 0.46 μM. 28774427
HL60 Antiparasitic assay 72 hrs Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, EC50 = 0.055 μM. 28803047
MOLT4 Antiparasitic assay 72 hrs Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay, EC50 = 0.17 μM. 28803047
Vero Cytotoxicity assay 72 hrs Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay, CC50 = 2.8 μM. 28803047
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.45 μM. 29236492
HEK293T Cytotoxicity assay 72 hrs Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.42 μM. 29469575
HCT8 Antiparasitic assay 48 hrs Antiparasitic activity against Cryptosporidium parvum BGF infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.55 μM. 29469575
HCT8 Antiparasitic assay 48 hrs Antiparasitic activity against Cryptosporidium parvum SPL infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.6 μM. 29469575
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.83 μM. 29469575
HCT8 Cytotoxicity assay Cytotoxicity against human HCT8 cells, EC50 = 6.1 μM. 29469575
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay, IC50 = 0.46 μM. 29533611
HEK293 Cytotoxicity assay 72 hrs Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay, IC50 = 0.4 μM. 29969262
A549 Cytotoxicity assay 48 hrs Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM. 30418763
PC3 Cytotoxicity assay 48 hrs Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM. 30418763
Click to View More Cell Line Experimental Data

Biological Activity

Description Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.
In vitro
In vitro

The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1]

Puromycin can be used for the selection of recombinant cells from noncultured cells. [2]
Cell Research Cell lines HepG2 cells 
Concentrations 1.6 μM
Incubation Time 72 h
Method

Cells were treated with different concentrations of drug for 72 h.
Experimental Result Images Methods Biomarkers Images PMID
Western blot Cyclin D1 / CDK4 PARP / Bcl-xL / Bcl-2 / p-Akt / Akt p53 / p21 / c-Myc 31022952
Growth inhibition assay Cell viability 31022952
In Vivo
In vivo

Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.[4]
Animal Research Animal Models Male Sprague-Dawley rats
Dosages 5 mg/100 g
Administration i.v.

Chemical Information & Solubility

Molecular Weight 544.43 Formula

C22H29N7O5.2HCl
CAS No. 58-58-2 SDF Download Puromycin 2HCl SDF
Smiles CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (183.67 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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