human colon carcinoma cell line HCT116 |
Function assay |
|
|
|
Concentration required for growth inhibition of human colon carcinoma cell line HCT116, IC50=6 nM. |
15537345 |
human PBMC |
Function assay |
|
24 h |
|
Suppression of IL2 production in human PBMC after 24 hrs by ELISA, IC50=16 nM. |
18585046 |
endothelial cells |
Function assay |
|
|
|
Effective dose against plasminogen activator activity stimulated by phorbol ester in endothelial cells, ED50 = 7.5 μM. |
8709095 |
Sf9 insect cells |
Function assay |
|
|
|
Inhibition of protein kinase C(1:1mixture of PKC alpha & beta) expressed in Sf9 insect cells, IC50 = 0.02 μM. |
9873605 |
LoVo cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against human colon cancer cell line (LoVo cells) using MTT assay, IC50 = 0.001 μM. |
11591505 |
umbilical vein endothelial cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against human umbilical vein endothelial cells (HUVECs) using MTT assay, IC50 = 0.004 μM. |
11591505 |
ST-486 cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against burkit lymphoma cell line (ST-486 cells) using MTT assay, IC50 = 0.007 μM. |
11591505 |
DLD-1 cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against human colon cancer cell line (DLD-1 cells) using MTT assay, IC50 = 0.009 μM. |
11591505 |
HEK293 cells |
Function assay |
|
|
|
Inhibition of PKC-beta II mediated IL-8 release from HEK293 cells by ELISA, IC50 = 0.077 μM. |
12941331 |
P19 cells |
Function assay |
|
|
|
Inhibition of Platelet-derived growth factor receptor in P19 cells, IC50 = 0.002 μM. |
15771419 |
P19 cells |
Function assay |
|
|
|
Inhibition of Platelet-derived growth factor receptor in P19 cells, IC50 = 0.002 μM. |
15771419 |
P19 cells |
Function assay |
|
|
|
Inhibition of Protein Kinase A in P19 cells, IC50 = 0.004 μM. |
15771419 |
P19 cells |
Function assay |
|
|
|
Inhibition of Calcium/calmodulin-dependent protein kinase type II in P19 cells, IC50 = 0.006 μM. |
15771419 |
P19 cells |
Function assay |
|
|
|
Inhibition of Cyclin-dependent kinase 1 in P19 cells, IC50 = 0.008 μM. |
15771419 |
P19 cells |
Function assay |
|
|
|
Inhibition of Vascular endothelial growth factor receptor in P19 cells, IC50 = 0.014 μM. |
15771419 |
HEK293 cells |
Function assay |
|
|
|
Inhibition of IL-8 release by HEK293 cells expressing PKC-beta2, IC50 = 0.077 μM. |
15771419 |
P19 cells |
Function assay |
|
|
|
Inhibition of Insulin receptor kinase in P19 cells, IC50 = 0.2 μM. |
15771419 |
P19 cells |
Function assay |
|
|
|
Inhibition of Casein kinase 1 in P19 cells, IC50 = 1.4 μM. |
15771419 |
FL5.12-Akt1 cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against FL5.12-Akt1 cells by MTT assay, IC50 = 0.29 μM. |
16403626 |
MiaPaCa-2 cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against MiaPaCa-2 cells by MTT assay, IC50 = 0.37 μM. |
16403626 |
FL5.12-Akt1 cells |
Function assay |
|
|
|
Activity against GSK3 in FL5.12-Akt1 cells, EC50 = 0.46 μM. |
16403626 |
MiaPaCa-2 cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against human MiaPaCa-2 cells, IC50 = 0.37 μM. |
16413780 |
FL5.12-Akt1 cells |
Function assay |
|
|
|
Activity against GSK3 phosphorylation in FL5.12-Akt1 cells, EC50 = 0.46 μM. |
16413780 |
Sf21 cells |
Function assay |
|
|
|
Inhibition of JAK3 expressed in Sf21 cells, IC50 = 0.006 μM. |
17088059 |
U937 cells |
Cytotoxicity assay |
|
|
|
Cytotoxicity against human U937 cells, IC50 = 2 μM. |
17088067 |
Sf9 cells |
Function assay |
|
1 min |
|
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 1 umol/L ATP, IC50 = 0.005 μM. |
17315853 |
Sf9 cells |
Function assay |
|
20 mins |
|
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 1 umol/L ATP, IC50 = 0.015 μM. |
17315853 |
Sf9 cells |
Function assay |
|
1 min |
|
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 10 umol/L ATP, IC50 = 0.036 μM. |
17315853 |
Sf9 cells |
Function assay |
|
20 mins |
|
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 10 umol/L ATP, IC50 = 0.083 μM. |
17315853 |
Sf9 cells |
Function assay |
|
1 min |
|
Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 100 umol/L ATP, IC50 = 0.27 μM. |
17315853 |
Sf9 cells |
Function assay |
|
20 mins |
|
Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 100 umol/L ATP, IC50 = 0.51 μM. |
17315853 |
A549 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B method, GI50 = 0.0024 μM. |
18484775 |
MDA-MB-231 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human MDA-MB-231 cells after 72 hrs by sulforhodamine B method, GI50 = 0.0071 μM. |
18484775 |
HT29 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human HT29 cells after 72 hrs by sulforhodamine B method, GI50 = 0.0109 μM. |
18484775 |
MCF7 cells |
Function assay |
|
|
|
Inhibition of PKA in human MCF7 cells by array-based fluorescence assay, Ki = 0.005 μM. |
18656369 |
MCF7 cells |
Function assay |
|
|
|
Inhibition of PKA in human MCF7 cells by array-based fluorescence assay, IC50 = 2 μM. |
18656369 |
Sf9 cells |
Function assay |
|
|
|
Inhibition of human Syk expressed in Sf9 cells, IC50 = 0.003 μM. |
18823784 |
Sf9 cells |
Function assay |
|
|
|
Inhibition of human ZAP70 expressed in Sf9 cells, IC50 = 0.053 μM. |
18823784 |
insect cells |
Function assay |
|
|
|
Inhibition of human recombinant Pim1 expressed in insect cells by HTRF, IC50 = 0.01 μM. |
19179076 |
HT-29 cells |
Apoptosis assay |
|
2 h |
|
Induction of apoptosis in human HT-29 cells assessed as change in mitochondrial transmembrane potential after 2 hrs by JC1 staining based fluorescence assay, ED50 = 0.0026 μM. |
19422206 |
insect Sf21 cells |
Function assay |
|
|
|
Inhibition of JAK3 expressed in insect Sf21 cells assessed as inhibition of biotinylated substrate phosphorylation, IC50 = 0.006 μM. |
19427203 |
Jurkat cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against JAK3 expressing IL2-stimulated human Jurkat cells, IC50 = 0.071 μM. |
19427203 |
Jurkat cells |
Antiproliferative activity assay |
|
|
|
Antiproliferative activity against JAK3 expressing human resting Jurkat cells, IC50 = 0.311 μM. |
19427203 |
HUE cells |
Function assay |
|
90 mins |
|
Inhibition of VEGFR2 in HUE cells assessed as inhibition of VEGF-induced autophosphorylation treated for 90 mins before VEGF challenge by ELISA, IC50 = 0.07 μM. |
20170163 |
Sf9 cells |
Function assay |
|
60 mins |
|
Inhibition of human recombinant GST-fused NIK expressed in Sf9 cells after 60 mins by radiometric protein kinase assay, IC50 = 2.3 μM. |
20580552 |
Sf21 cells |
Function assay |
30 uM |
|
|
Inhibition of human CHK2 kinase expressed in insect Sf21 cells using phospho-CREBtide as substrate at 30 uM, IC50 = 0.027 μM. |
21334796 |
Sf21 cells |
Function assay |
30 uM |
|
|
Inhibition of human recombinant IKK-alpha expressed in insect Sf21 cells using phospho-Ulight-IkappaB-alpha as substrate at 30 uM, IC50 = 0.039 μM. |
21334796 |
Sf21 cells |
Function assay |
30 uM |
|
|
Inhibition of human recombinant IKK-beta expressed in insect Sf21 cells using phospho-Ulight-IkappaB-alpha as substrate at 30 uM, IC50 = 0.49 μM. |
21334796 |
HeLa cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay, IC50 = 0.000004 μM. |
21388191 |
MCF7 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay, IC50 = 0.05 μM. |
21388191 |
HT-29 cells |
Function assay |
|
2 h |
|
Effect on mitochondrial membrane potential in human HT-29 cells after 2 hrs using JC-1 staining by fluorescence assay, IC50 = 0.002 μM. |
21428375 |
HeLa cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay, IC50 = 0.000004 μM. |
21488655 |
MCF7 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay, IC50 = 0.05 μM. |
21488655 |
HT-29 cells |
Function assay |
|
|
|
Inhibition of mitochondrial membrane potential in human HT-29 cells using JC1 dye staining by fluorescence plate reader assay, IC50 = 0.0025 μM. |
21513293 |
SF268 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human SF268 cells after 48 hrs by SRB assay, GI50 = 0.044 μM. |
21513294 |
CHOK1 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human CHOK1 cells after 48 hrs by SRB assay, GI50 = 0.13 μM. |
21513294 |
H460 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human H460 cells after 48 hrs by SRB assay, GI50 = 3.6 μM. |
21513294 |
HT-29 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human HT-29 cells after 48 hrs by SRB assay, GI50 = 3.6 μM. |
21513294 |
MCF7 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay, GI50 = 11 μM. |
21513294 |
SW1116 cells |
Function assay |
|
24 h |
|
Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay, IC50 = 8.32 μM. |
21962523 |
HT-29 cells |
Apoptosis assay |
|
2 h |
|
Induction of apoptosis in human HT-29 cells assessed reduction of mitochondrial membrane potential after 2 hrs by using JC1 staining by fluorescence cell-based assay, ED50 = 0.0026 μM. |
21973101 |
LoVo cells |
Antiproliferative activity assay |
|
48 to 72 h |
|
Antiproliferative activity against human LoVo cells after 48 to 72 hrs by MTT assay, IC50 = 0.001 μM. |
22182929 |
ST486 cells |
Antiproliferative activity assay |
|
48 to 72 h |
|
Antiproliferative activity against human ST486 cells after 48 to 72 hrs by MTT assay, IC50 = 0.007 μM. |
22182929 |
DLD1 cells |
Antiproliferative activity assay |
|
48 to 72 h |
|
Antiproliferative activity against human DLD1 cells after 48 to 72 hrs by MTT assay, IC50 = 0.009 μM. |
22182929 |
B16F10 cells |
Antiproliferative activity assay |
|
48 h |
|
Antiproliferative activity against mouse B16F10 cells after 48 hrs by MTT assay, IC50 = 2.82 μM. |
22503364 |
MCF7 cells |
Antiproliferative activity assay |
|
48 h |
|
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay, IC50 = 3.07 μM. |
22503364 |
A431 cells |
Cytotoxicity assay |
|
24 h |
|
Cytotoxicity against human A431 cells after 24 hrs using Annexin VeFITC/propidium iodide staining by MTT assay, IC50 = 0.07 μM. |
22541051 |
MCF7 cells |
Cytotoxicity assay |
|
24 h |
|
Cytotoxicity against human MCF7 cells after 24 hrs using Annexin VeFITC/propidium iodide staining by MTT assay, IC50 = 0.18 μM. |
22541051 |
BJ cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human BJ cells after 72 hrs by Calcein AM assay, IC50 = 0.002 μM. |
22921081 |
CEM cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human CEM cells after 72 hrs by Calcein AM assay, IC50 = 0.023 μM. |
22921081 |
MCF7 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay, IC50 = 0.064 μM. |
22921081 |
HeLa cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human HeLa cells after 72 hrs by Calcein AM assay, IC50 = 0.175 μM. |
22921081 |
HepG2 cells |
Function assay |
|
24 h |
|
Inhibition of telomerase in human HepG2 cells after 24 hrs by TRAP-PCR-ELISA assay, IC50 = 4.14 μM. |
23018096 |
SW1116 cells |
Antiproliferative activity assay |
|
48 h |
|
Antiproliferative activity against human SW1116 cells after 48 hrs by MTT method, IC50 = 4.95 μM. |
23018096 |
HepG2 cells |
Antiproliferative activity assay |
|
48 h |
|
Antiproliferative activity against human HepG2 cells after 48 hrs by MTT method, IC50 = 6.73 μM. |
23018096 |
BGC823 cells |
Antiproliferative activity assay |
|
48 h |
|
Antiproliferative activity against human BGC823 cells after 48 hrs by MTT method, IC50 = 6.83 μM. |
23018096 |
HeLa cells |
Antiproliferative activity assay |
|
48 h |
|
Antiproliferative activity against human HeLa cells after 48 hrs by MTT method, IC50 = 9.12 μM. |
23018096 |
HCT116 cells |
Function assay |
|
|
|
Inhibition of TNIK-mediated TCF4/beta-casein transcription in human HCT116 cells by beta-lactamase reporter gene assay, IC50 = 0.039 μM. |
23232060 |
HepG2 cells |
Function assay |
|
48 h |
|
Inhibition of telomerase in human HepG2 cells after 48 hrs by TRAP-PCR-ELISA, IC50 = 8.3 μM. |
23279864 |
SW1116 cells |
Function assay |
|
24 h |
|
Inhibition of telomerase in human SW1116 cells after 24 hrs by TRAP-PCR-ELISA assay, IC50 = 4.18 μM. |
24286761 |
KE-97 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human KE-97 cells after 72 hrs by CellTitre-Glo luminescent cell viability assay, IC50 = 0.13 μM. |
24328283 |
Jurkat cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human Jurkat cells after 72 hrs by CellTitre-Glo luminescent cell viability assay, IC50 = 0.14 μM. |
24328283 |
HuH7 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human HuH7 cells after 72 hrs by CellTitre-Glo luminescent cell viability assay, IC50 = 0.23 μM. |
24328283 |
BGC823 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human BGC823 cells after 72 hrs by CellTitre-Glo luminescent cell viability assay, IC50 = 0.38 μM. |
24328283 |
MCF7 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human MCF7 cells after 72 hrs by CellTitre-Glo luminescent cell viability assay, IC50 = 0.52 μM. |
24328283 |
NIH/3T3 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay, IC50 = 0.2 μM. |
24361521 |
A2780 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human A2780 cells after 96 hrs by SRB assay, IC50 = 0.2 μM. |
24361521 |
HT-29 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay, IC50 = 0.2 μM. |
24361521 |
8505C cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human 8505C cells after 96 hrs by SRB assay, IC50 = 0.2 μM. |
24361521 |
518A2 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay, IC50 = 0.2 μM. |
24361521 |
MCF7 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay, IC50 = 0.4 μM. |
24361521 |
A549 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human A549 cells after 96 hrs by SRB assay, IC50 = 0.6 μM. |
24361521 |
V79 MZ cells |
Function assay |
|
|
|
Inhibition of human aldosterone synthase expressed in V79 MZ cells assessed as inhibition of aldosterone synthesis, IC50 = 0.011 μM. |
24422519 |
HEK293 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against HEK293 cells after 72 hrs by CellTiterGlo assay, IC50 = 0.056 μM. |
24763262 |
HeLa cells |
Antiproliferative activity assay |
|
24 to 48 h |
|
Antiproliferative activity against human HeLa cells after 24 to 48 hrs by CCK-8 assay, IC50 = 2.72 μM. |
24792811 |
A549 cells |
Antiproliferative activity assay |
|
24 to 48 h |
|
Antiproliferative activity against human A549 cells after 24 to 48 hrs by CCK-8 assay, IC50 = 3.05 μM. |
24792811 |
HepG2 cells |
Cytotoxicity assay |
|
|
|
Cytotoxicity against human HepG2 cells, EC50 = 2 μM. |
25316317 |
Raji cells |
Cytotoxicity assay |
|
|
|
Cytotoxicity against human Raji cells, EC50 = 2 μM. |
25316317 |
BJ cells |
Cytotoxicity assay |
|
|
|
Cytotoxicity against human BJ cells, EC50 = 2 μM. |
25316317 |
HEK293 cells |
Cytotoxicity assay |
|
|
|
Cytotoxicity against HEK293 cells, EC50 = 2 μM. |
25316317 |
A549 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human A549 cells after 48 hrs by MTT assay, IC50 = 0.02 μM. |
25825934 |
HeLa cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay, IC50 = 0.025 μM. |
25825934 |
PC3 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human PC3 cells after 48 hrs by MTT assay, IC50 = 0.031 μM. |
25825934 |
MGC-803 cells |
Function assay |
|
24 h |
|
Inhibition of telomerase in human MGC-803 cells after 24 hrs by TRAP-PCR-ELISA assay, IC50 = 8.97 μM. |
26900656 |
Sf9 insect cells |
Function assay |
|
10 mins |
|
Inhibition of N-terminal GST tagged recombinant full-length human CHK1 expressed in baculovirus Sf9 insect cells after 10 mins by luminescent ADP-Glo assay, IC50 = 0.0012 μM. |
27089211 |
Sf21 cells |
Function assay |
|
60 mins |
|
Inhibition of human recombinant JAK2 expressed in Sf21 cells assessed as reduction in Ulight-CAGAGAIETDKEYYTVKD phosphorylation pre-incubated before substrate addition and measured after 60 mins by LANCE detection method, IC50 = 0.002 μM. |
27137359 |
Neuro2a cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against mouse Neuro2a cells assessed as cell viability after 72 hrs by MTT assay, IC50 = 0.0113 μM. |
28152427 |
MDA-MB-231 cells |
Function assay |
|
16 h |
|
Inhibition of retinoblastoma protein phosphorylation in human MDA-MB-231 cells after 16 hrs by Hoechst 33342 staining based fluorescence assay, IC50 = 0.066 μM. |
28431342 |
Sf9 insect cells |
Function assay |
|
20 mins |
|
Inhibition of recombinant human N-terminally GST-tagged EGFR L858R/T790M/C797S triple mutant expressed in baculovirus in Sf9 insect cells preincubated for 20 mins followed by addition of [33P]-ATP measured after 2 hrs by filter-binding method, IC50 = 0.00029 μM. |
28482151 |
HEK293 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against HEK293 cells assessed as decrease in cell viability after 72 hrs by CellTiter Glo luminescent assay, CC50 = 0.00354 μM. |
28624701 |
BT549 cells |
Cytotoxicity assay |
|
24 h |
|
Cytotoxic activity against human BT549 cells incubated for 24 hrs by MTT assay, IC50 = 0.08 μM. |
28705432 |
MDA-MB-468 cells |
Cytotoxicity assay |
|
24 h |
|
Cytotoxic activity against human MDA-MB-468 cells incubated for 24 hrs by MTT assay, IC50 = 0.15 μM. |
28705432 |
MDA-MB-231 cells |
Cytotoxicity assay |
|
24 h |
|
Cytotoxic activity against human MDA-MB-231 cells incubated for 24 hrs by MTT assay, IC50 = 0.24 μM. |
28705432 |
Vero cells |
Cytotoxicity assay |
|
2 days |
|
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 2 days by cell-titer-glo luminescent assay, TC50 = 0.0024 μM. |
28763645 |
L929 cells |
Antiproliferative activity assay |
|
5 days |
|
Antiproliferative activity against mouse L929 cells after 5 days by WST-1 assay, IC50 = 0.2 μM. |
28956915 |
KB-3-1 cells |
Antiproliferative activity assay |
|
5 days |
|
Antiproliferative activity against human KB-3-1 cells after 5 days by WST-1 assay, IC50 = 0.2 μM. |
28956915 |
MCF7 cells |
Antiproliferative activity assay |
|
5 days |
|
Antiproliferative activity against human MCF7 cells after 5 days by WST-1 assay, IC50 = 1.8 μM. |
28956915 |
FS4-LTM cells |
Antiproliferative activity assay |
|
24 h |
|
Antiproliferative activity against human FS4-LTM cells after 24 hrs by WST-1 assay, IC50 = 2.8 μM. |
28956915 |
Vero cells |
Cytotoxicity assay |
|
2 days |
|
Cytotoxicity against African green monkey Vero cells after 2 days by CellTiter-Glo luminescent cell viability assay, IC50 = 0.0024 μM. |
28993106 |
HepG2 cells |
Cytotoxicity assay |
100 uM |
3 days |
|
Cytotoxicity against human HepG2 cells at 100 uM after 3 days by CellTiter-Glo luminescent cell viability assay, IC50 = 0.018 μM. |
28993106 |
NIH/3T3 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay, EC50 = 0.008 μM. |
29197730 |
518A2 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay, EC50 = 0.03 μM. |
29197730 |
A549 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human A549 cells after 96 hrs by SRB assay, EC50 = 0.04 μM. |
29197730 |
MCF7 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay, EC50 = 0.1 μM. |
29197730 |
A2780 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human A2780 cells after 96 hrs by SRB assay, EC50 = 0.12 μM. |
29197730 |
HT-29 cells |
Cytotoxicity assay |
|
96 h |
|
Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay, EC50 = 0.15 μM. |
29197730 |
PC3 cells |
Cytotoxicity assay |
|
|
|
Cytotoxicity against human PC3 cells by sulforhodamine B assay, IC50 = 0.017 μM. |
29389122 |
HCT116 cells |
Cytotoxicity assay |
|
|
|
Cytotoxicity against human HCT116 cells by sulforhodamine B assay, IC50 = 0.055 μM. |
29389122 |
insect cells |
Function assay |
|
30 mins |
|
Inhibition of recombinant human PDGFRbeta expressed in insect cells using Ulight-Poly GAT[EAY(1:1:1)]n as substrate after 30 mins by LANCE method, IC50 = 0.00076 μM. |
29549836 |
Sf9 insect cells |
Function assay |
|
60 mins |
|
Inhibition of recombinant human KDR expressed in Sf9 insect cells using Ulight-CAGAGAIETDKEYYTVKD as substrate after 60 mins by LANCE method, IC50 = 0.0021 μM. |
29549836 |
insect cells |
Function assay |
|
10 mins |
|
Inhibition of recombinant human Src expressed in insect cells using Ulight-Poly GAT[EAY(1:1:1)]n as substrate after 10 mins by LANCE method, IC50 = 0.005 μM. |
29549836 |
insect cells |
Function assay |
|
15 mins |
|
Inhibition of recombinant human CDK1/Cyclin B expressed in insect cells using Ulight-CFFKNIVTPRTPPPSQGK-amide as substrate after 15 mins by LANCE method, IC50 = 0.025 μM. |
29549836 |
insect cells |
Function assay |
|
15 mins |
|
Inhibition of recombinant human CDK1/Cyclin B expressed in insect cells using Ulight-CFFKNIVTPRTPPPSQGK-amide as substrate after 15 mins by LANCE method, IC50 = 0.025 μM. |
29549836 |
Sf9 insect cells |
Function assay |
|
15 mins |
|
Inhibition of recombinant human Flt1 expressed in Sf9 insect cells using Ulight-TK peptide as substrate after 15 mins by LANCE method, IC50 = 0.035 μM. |
29549836 |
Sf21 insect cells |
Function assay |
|
15 mins |
|
Inhibition of recombinant human Aurora-A expressed in Sf21 insect cells using Ulight-RRRSLLE as substrate after 15 mins by LANCE method, IC50 = 0.039 μM. |
29549836 |
insect cells |
Function assay |
|
60 mins |
|
Inhibition of recombinant human AKT1 expressed in insect cells using CREBtide as substrate after 60 mins by LANCE method, IC50 = 0.056 μM. |
29549836 |
Sf9 insect cells |
Function assay |
|
60 mins |
|
Inhibition of recombinant human FAK expressed in Sf9 insect cells using Ulight-TK peptide as substrate after 60 mins by LANCE method, IC50 = 0.062 μM. |
29549836 |
insect cells |
Function assay |
|
60 mins |
|
Inhibition of recombinant human ABL expressed in insect cells using Ulight-TK peptide as substrate after 60 mins by LANCE method, IC50 = 0.29 μM. |
29549836 |
insect cells |
Function assay |
|
60 mins |
|
Inhibition of recombinant human c-MET expressed in insect cells using Ulight-CAGAGAIETDKEYYTVKD as substrate after 60 mins by LANCE method, IC50 = 0.31 μM. |
29549836 |
PC3 cells |
Cytotoxicity assay |
|
72 h |
|
Cytotoxicity against human PC3 cells after 72 hrs by SRB assay, IC50 = 0.039 μM. |
29558119 |
Sf9 cells |
Function assay |
|
45 mins |
|
Inhibition of recombinant human C-terminal His-tagged/N-terminal GST-tagged EGFR (668 to 1210 residues) expressed in baculovirus infected Sf9 cells using Poly-(Glu, Tyr) as substrate measured after 45 minutes by Kinase-Glo luminescent assay, IC50 = 0.047 μM. |
29902719 |
Sf9 cells |
Function assay |
|
45 mins |
|
Inhibition of recombinant human N-terminal GST-tagged HER2 (679 to 1255 residues) expressed in baculovirus infected Sf9 cells using Poly-(Glu, Tyr) as substrate measured after 45 minutes by Kinase-Glo luminescent assay, IC50 = 0.0608 μM. |
29902719 |
HT-29 cells |
Function assay |
|
3 h |
|
Inhibition of mitochondrial membrane potential in human HT-29 cells after 3 hrs by JC-1 staining based fluorescence assay, IC50 = 0.02 μM. |
30057155 |
Sf9 cells |
Function assay |
|
|
|
Inhibition of N-terminal FLAG-tagged human full-length CDK12 (1 to 1490 residues)/N-terminal His-tagged CycK (1 to 580 residues) expressed in Sf9 cells assessed as reduction in ATP-dependent ULight-4E-BP1 (Thr37/Thr46) substrate peptide phosphorylation pr, IC50 = 0.053 μM. |
30067358 |
Sf9 cells |
Function assay |
|
|
|
Inhibition of N-terminal FLAG-tagged human full-length CDK12 (1 to 1490 residues)/N-terminal His-tagged CycK (1 to 580 residues) expressed in Sf9 cells assessed as reduction in ATP-dependent ULight-4E-BP1 (Thr37/Thr46) substrate peptide phosphorylation pr, IC50 = 0.053 μM. |
30067358 |
Sf9 cells |
Function assay |
|
|
|
Inhibition of N-terminal FLAG-tagged human full-length CDK13 (1 to 1512 residues)/N-terminal His-tagged CycK (1 to 580 residues) expressed in Sf9 cells assessed as reduction in ATP-dependent ULight-4E-BP1 (Thr37/Thr46) substrate peptide phosphorylation pr, IC50 = 0.057 μM. |
30067358 |
Sf9 cells |
Function assay |
|
|
|
Inhibition of N-terminal FLAG-tagged human full-length CDK13 (1 to 1512 residues)/N-terminal His-tagged CycK (1 to 580 residues) expressed in Sf9 cells assessed as reduction in ATP-dependent ULight-4E-BP1 (Thr37/Thr46) substrate peptide phosphorylation pr, IC50 = 0.057 μM. |
30067358 |
HCT116 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human HCT116 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 0.03 μM. |
30106291 |
Huh7.5 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human Huh7.5 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 0.6 μM. |
30106291 |
K562 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human K562 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 1.7 μM. |
30106291 |
LO2 cells |
Cytotoxicity assay |
|
48 h |
|
Cytotoxicity against human LO2 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50 = 9.5 μM. |
30106291 |