Home PI3K/Akt/mTOR Antineoplastic and Immunosuppressive Antibiotics inhibitor - Akt inhibitor - MEK inhibitor - Apoptosis related activator - HCV inhibitor - Autophagy activator Honokiol For research use only.

Honokiol

Synonyms: NSC 293100

Honokiol is the active principle of magnolia extract that inhibits Akt-phosphorylation and promotes ERK1/2 phosphorylation. Honokiol causes G0/G1 phase arrest, induces apoptosis, and autophagy via the ROS/ERK1/2 signaling pathway. Honokiol inhibits hepatitis C virus (HCV) infection. Phase 3.

Honokiol Chemical Structure

Honokiol Chemical Structure

CAS No. 35354-74-6

Purity & Quality Control

Honokiol Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human UACC-903 cells Cytotoxicity assay Cytotoxicity against human UACC-903 cells after 72 hrs by MTS assay, IC50=5.1 μM 22533983
Vero E6 cells Function assay Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA, EC50=6.5 μM 17663539
human UACC-903 cells Cytotoxicity assay Cytotoxicity against human UACC-903 cells after 24 hrs by MTS assay, IC50=7.45 μM 22533983
human A549 cells Cytotoxicity assay Cytotoxicity against human A549 cells after 72 hrs by MTS assay, IC50=7.75 μM 22533983
human CEM cells Cytotoxicity assay Cytotoxicity against human CEM cells, IC50=10.9 μM 17587572
HEK293 cells Function assay Agonist activity at RXRalpha in HEK293 cells assessed as transcriptional activation after 48 hrs by luciferase reporter gene assay, EC50=11.8 μM 20695472
human A549 cell Cytotoxicity assay 24 h Cytotoxicity against human A549 cells after 24 hrs by MTS assay, IC50=12.51 μM 22533983
human HT-29 cells Cytotoxicity assay 72 h Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay, IC50=13.24 μM 22533983
human PBM cells Cytotoxicity assay Cytotoxicity against human PBM cells, IC50=16.1 μM 17587572
Hep-G2 cells Cytotoxicity assay Cytotoxicity of compound against human liver tumor cell line (Hep-G2) was determined, IC50=16.5 μM 15582432
human HepG2 cells Proliferation assay 24 h Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay, IC50=16.5 μM 21853991
human K562 cells Proliferation assay Antiproliferative activity against human K562 cells by MTT assay, IC50=21.1 μM 19589678
Vero cells Cytotoxicity assay Cytotoxicity against Vero cells, IC50=22.5 μM 16722664
human A2780 cells Proliferation assay Antiproliferative activity against cisplatin-sensitive human A2780 cells by MTT assay, IC50=30.5 μM 19589678
human SPC-A1 cells Proliferation assay Antiproliferative activity against human SPC-A1 cells by MTT assay, IC50=36.1 μM 19589678
HUVEC cells Function assay 20 μM 24 h Antimigratory activity against human HUVEC cells at 20 uM after 24 hrs by wound-healing assay 21853991
HEK293 cells Function assay 24-48 h Agonist activity at human RXR-alpha expressed in HEK293 cells coexpressing with pCMX-beta-gal after 24 to 48 hrs by luciferase reporter gene assay 24959987
human SH-SY5Y cells Function assay 10 μM 30 mins Neuroprotective activity in human SH-SY5Y cells assessed as inhibition of CHP and TBHP-induced cell death at 10 uM incubated for 30 mins prior to challenge measured after 3 hrs by MTT assay 22142539
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Biological Activity

Description Honokiol is the active principle of magnolia extract that inhibits Akt-phosphorylation and promotes ERK1/2 phosphorylation. Honokiol causes G0/G1 phase arrest, induces apoptosis, and autophagy via the ROS/ERK1/2 signaling pathway. Honokiol inhibits hepatitis C virus (HCV) infection. Phase 3.
Targets
Akt-phosphorylation [1] MEK [8]
In vitro
In vitro

Honokiol shows pro-apoptotic effects in melanoma, sarcoma, myeloma, leukemia, bladder, lung, prostate, oral squamous cell carcinoma and colon cancer cell lines. Honokiol is effective on inducing apoptosis in SVR angiosarcoma cells. Treatment of SVR cells with honokiol causes decreased phosphorylation of MAP kinase, akt, and c-src. In addition, honokiol potentiates TRAIL-mediated apoptosis, and honokiol cytotoxicity is partially abrogated by neutralizing antibodies to TRAIL. Honokiol also has direct antiangiogenic activity, in that honokiol blocks the phosphorylation and rac activation due to VEGF-VEGFR2 interactions. [1] Honokiol causes apoptosis in CLL cells through activation of caspase 8, followed by caspase 9 and 3 activation. Honokiol prevents interleukin-4-mediated survival of CLL cells, and potentiats the cytotoxicity of CB-1348, FaraA, and 2-CdA. [3] Honokiol kills myeloma cells from relapsed patients at doses that does not kill PBMCs. Caspase 3, 7, 8, and 9 are induced by honokiol treatment, as well as PARP cleavage. [2] Honokiol is found to induce apoptosis in the colon cancer cell lines RKO. [4] Honokiol potentiates apoptosis, suppresses osteoclastogenesis, and inhibits invasion through modulation of nuclear factor-kappaB activation pathway. [5] Honokiol may act as a potent anti-inflammatory agent with multipotential activities due to an inhibitory effect on the PI3K/Akt pathway. [6]
Experimental Result Images Methods Biomarkers Images PMID
Western blot p-EGFR / p-AKT / p-STAT3 / p-ERK / p-GSK3α/β / Bax / p-pRb / p-BAD / IκBα / CDK2 / CDK4 / Cyclin D1 p-Lyn / Lyn / p-PI3K 27458163
Immunofluorescence Vimentin / Occludin IκBα / NF-κB p65 24508063
In Vivo
In vivo

Honokiol is highly effective against SVR angiosarcoma in nude mice. [1] Honokiol inhibits the growth of RKO cells in murine xenografts. [4] Honokiol prevents the growth of MDA-MD-231 breast cancer cells in murine xenografts. [7]

Chemical Information & Solubility

Molecular Weight 266.334 Formula

C18H18O2
CAS No. 35354-74-6 SDF Download Honokiol SDF
Smiles C=CCC1=CC(=C(C=C1)O)C2=CC(=C(C=C2)O)CC=C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 53 mg/mL ( (198.99 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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