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Technical Data
| Formula | C22H29N7O5.2HCl |
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| Molecular Weight | 544.43 | CAS No. | 58-58-2 | |
| Solubility (25°C)* | In vitro | DMSO | 100 mg/mL (183.67 mM) | |
| Water | 100 mg/mL (183.67 mM) | |||
| Ethanol | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation. |
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| In vitro | The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. [1] Puromycin can be used for the selection of recombinant cells from noncultured cells. [2] |
| In vivo | Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.[4] |
Protocol (from reference)
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References
Selleck's Puromycin 2HCl has been cited by 209 publications
| FOSL1 transcriptionally dictates the Warburg effect and enhances chemoresistance in triple-negative breast cancer [ J Transl Med, 2025, 23(1):1] | PubMed: 39748430 |
| PRRSV-2 nsp2 Ignites NLRP3 inflammasome through IKKβ-dependent dispersed trans-Golgi network translocation [ PLoS Pathog, 2025, 21(1):e1012915] | PubMed: 39869629 |
| Neoadjuvant PARPi or chemotherapy in ovarian cancer informs targeting effector Treg cells for homologous-recombination-deficient tumors [ Cell, 2024, 187(18):4905-4925.e24] | PubMed: 38971151 |
| Neoadjuvant PARPi or chemotherapy in ovarian cancer informs targeting effector Treg cells for homologous-recombination-deficient tumors [ Cell, 2024, S0092-8674(24)00653-6] | PubMed: 38971151 |
| Single-cell exome sequencing reveals polyclonal seeding and TRPS1 mutations in colon cancer metastasis [ Signal Transduct Target Ther, 2024, 9(1):247] | PubMed: 39307879 |
| Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis [ Signal Transduct Target Ther, 2024, 9(1):58] | PubMed: 38438346 |
| The p-MYH9/USP22/HIF-1α axis promotes lenvatinib resistance and cancer stemness in hepatocellular carcinoma [ Signal Transduct Target Ther, 2024, 9(1):249] | PubMed: 39300073 |
| GRP75 triggers white adipose tissue browning to promote cancer-associated cachexia [ Signal Transduct Target Ther, 2024, 9(1):253] | PubMed: 39327432 |
| Dual impacts of serine/glycine-free diet in enhancing antitumor immunity and promoting evasion via PD-L1 lactylation [ Cell Metab, 2024, S1550-4131(24)00415-7] | PubMed: 39577415 |
| O-GlcNAcylation Facilitates the Interaction between Keratin 18 and Isocitrate Dehydrogenases and Potentially Influencing Cholangiocarcinoma Progression [ ACS Cent Sci, 2024, 10(5):1065-1083] | PubMed: 38799671 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.