Nigericin sodium salt

Catalog No.S6653 Batch:S665302

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Technical Data

Formula

C40H67NaO11
Molecular Weight 746.94 CAS No. 28643-80-3
Solubility (25°C)* In vitro Ethanol 65 mg/mL (87.02 mM)
DMSO 50 mg/mL (66.93 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution (main for i.p.)
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Nigericin sodium salt is an antibiotic derived from Streptomyces hygroscopicus that works by acting as an H+, K+, and Pb2+ ionophore.Nigericin can activate NLRP3 inflammasome to induce pro-inflammatory and immunostimulatory processes.
Targets
Potassium channel [1] Hydrogen channel [1] Pb 2+ [1] NLRP3 [2]
In vitro

In vitro model of tumor heterogeneity the antitumor effects of Nigericin (NIG) that works by acting as an H+, K+, and Pb2+ ionophore. NIG decreased the viability and clonogenicity of H460 cells, as well as their ability to migrate and invade[1] .

Using a combination of live-cell imaging and biochemical approaches, we demonstrate that prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death. [2]
In vivo

Nigericin is a NOD-like receptor protein 3 (NLRP3) activator.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    H460 cells

  • Concentrations

    1 µM

  • Incubation Time

    24 h

  • Method

    H460 cells are seeded in 8-µm pore size Transwell® Inserts in RPMI 1640 (without FBS) with or without drugs (DMSO as control) or NIG (1µM). Inserts are placed in 24-well plates with RPMI 1640 containing 10% FBS in the presence of DMSO (control) or NIG (1 µM).
Animal Study:

[3]
  • Animal Models

    Male C57BL/6 mice

  • Dosages

    4 mg/kg

  • Administration

    i.p.

Selleck's Nigericin sodium salt has been cited by 29 publications

Chinese medicinal formula Fu Xin decoction against chronic heart failure by inhibiting the NLRP3/caspase-1/GSDMD pyroptotic pathway [ Biomed Pharmacother, 2024, 174:116548] PubMed: 38599064
Pre-treatment with notoginsenoside R1 from Panax notoginseng protects against high-altitude-induced pulmonary edema by inhibiting pyroptosis through the NLRP3/caspase-1/GSDMD pathway [ Biomed Pharmacother, 2024, 180:117512] PubMed: 39353320
Methionine deficiency inhibited pyroptosis in primary hepatocytes of grass carp (Ctenopharyngodon idella): possibly via activating the ROS-AMPK-autophagy axis [ J Anim Sci Biotechnol, 2024, 15(1):116] PubMed: 39218924
3'-Deoxyadenosin alleviates methamphetamine-induced aberrant synaptic plasticity and seeking behavior by inhibiting the NLRP3 inflammasome [ Neural Regen Res, 2024, 19(10):2270-2280] PubMed: 38488561
ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2 [ EMBO J, 2023, e113118.] PubMed: 37646198
Lipocalin-2-mediated astrocyte pyroptosis promotes neuroinflammatory injury via NLRP3 inflammasome activation in cerebral ischemia/reperfusion injury [ J Neuroinflammation, 2023, 20(1):148] PubMed: 37353794
Stellate ganglion block ameliorated central post-stroke pain with comorbid anxiety and depression through inhibiting HIF-1α/NLRP3 signaling following thalamic hemorrhagic stroke [ J Neuroinflammation, 2023, 20(1):82] PubMed: 36944982
A novel sorbicillinoid compound as a potent anti-inflammation agent through inducing NLRP3 protein degradation [ Br J Pharmacol, 2023, 10.1111/bph.16058] PubMed: 36788033
Takinib inhibits microglial M1 polarization and oxidative damage after subarachnoid hemorrhage by targeting TAK1-dependent NLRP3 inflammasome signaling pathway [ Front Immunol, 2023, 14:1266315] PubMed: 38035075
Hydrogen-rich saline regulates NLRP3 inflammasome activation in sepsis-associated encephalopathy rat model [ Int Immunopharmacol, 2023, 123:110758] PubMed: 37556997

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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