Home Stem Cells & Wnt Wnt/beta-catenin inhibitor MSAB For research use only.

MSAB

MSAB is a selective inhibitor of Wnt/β-catenin signaling that binds to β-catenin, promoting its degradation, and specifically downregulates Wnt/β-catenin target genes. MSAB shows potent anti-tumor effects.

MSAB Chemical Structure

MSAB Chemical Structure

CAS No. 173436-66-3

Purity & Quality Control

Batch: S690101 DMSO]61 mg/mL]false]Ethanol]10 mg/mL]false]Water]Insoluble]false Purity: 99.85%
99.85

MSAB Related Products

Signaling Pathway

Wnt/beta-catenin Signaling Pathways

Wnt/beta-catenin Signaling Pathway

Biological Activity

Description MSAB is a selective inhibitor of Wnt/β-catenin signaling that binds to β-catenin, promoting its degradation, and specifically downregulates Wnt/β-catenin target genes. MSAB shows potent anti-tumor effects.
Targets
β-catenin [1]
In vitro
In vitro

MSAB impedes RAW264.7 cells and human PMBCs differentiating into OCs. MSAB decreases the expression of β‐catenin in RAW264.7 and ARP1 WT cells.[2]
Cell Research Cell lines Human blood mononuclear cells (PBMCs), Human MM cell lines, HEK293, RAW264.7 cells
Concentrations 0, 2.5, 5, 10, 20, 40 μM
Incubation Time 6 days, 15 days
Method

RAW264 cells are seeded in a 24‐well plate at the density of 3,000 cells/well. Humen PBMCs cells are seeded in a 24‐well plate at the density of 1 × 106 cells/well. After 6 days (RAW264 cells) or 15 days (PBMCs), the degree of osteoclast differentiation is determined by detecting the TRAP activity of the cells.
In Vivo
In vivo

MSAB (10-20 mg/kg; i.p. daily for 2 weeks) inhibits tumor growth of Wnt-dependent cancer cells in xenograft mice and MMTV-Wnt1 transgenic mice.[3]

Chemical Information & Solubility

Molecular Weight 305.35 Formula

C15H15NO4S
CAS No. 173436-66-3 SDF --
Smiles COC(=O)C1=CC=CC(=C1)N[S](=O)(=O)C2=CC=C(C)C=C2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 61 mg/mL ( (199.77 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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