Home Stem Cells & Wnt Wnt/beta-catenin activator Methyl Vanillate For research use only.

Methyl Vanillate

Methyl Vanillate, one of the ingredients in Hovenia dulcis Thunb, activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro.

Methyl Vanillate Chemical Structure

Methyl Vanillate Chemical Structure

CAS No. 3943-74-6

Purity & Quality Control

Batch: S363001 DMSO]36 mg/mL]false]Ethanol]36 mg/mL]false]Water]12 mg/mL]false Purity: 99.92%
99.92

Methyl Vanillate Related Products

Signaling Pathway

Wnt/beta-catenin Signaling Pathways

Wnt/beta-catenin Signaling Pathway

Biological Activity

Description Methyl Vanillate, one of the ingredients in Hovenia dulcis Thunb, activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro.
Targets
Wnt/β-catenin [1]
In vitro
In vitro Methyl vanillate (MV) activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro. It increases the expression of differentiation markers RUNX2, BMP2, ALP, and OCN in a dose-dependent manner. MV decreases and increases the expression of RANKL and OPG, respectively. Methyl vanillate dose-dependently elevates ALP activity in calvarial osteoblasts[1].
Cell Research Cell lines Calvarial osteoblasts
Concentrations 10 and 20 μM
Incubation Time 24 h
Method Calvarial osteoblasts are seeded onto a coverslip in 12-well plates at a density of 3×104 cells/well. After 24 h, HDT extract or MV is added for another 24 h and the cells are subsequently fixed with 4% PFA for 10 min. Cells are permeabilized with 0.2% Triton X-100 for 15 min and incubated for 30 min with 5% bovine serum albumin (BSA) blocking solution. The cells are incubated with the primary antibody (β-catenin) overnight at 4°C and then washed with PBS three times. Cells are then incubated with Alexa Fluor 502-conjugated IgG secondary antibody is incubated for 1 h, followed by incubation with DAPI for 5 min. The cells are mounted in Gel/Mount media. The fluorescence signal is captured using confocal microscopy.
In Vivo
In vivo Methyl vanillate rescues trabecular or cortical femoral bone loss in the ovariectomized mice without inducing any significant weight changes or abnormality in liver tissue when administrated orally[1].
Animal Research Animal Models ICR mice
Dosages 25 and 100 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 182.17 Formula

C9H10O4
CAS No. 3943-74-6 SDF Download Methyl Vanillate SDF
Smiles COC1=C(C=CC(=C1)C(=O)OC)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 36 mg/mL ( (197.61 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 36 mg/mL

Water : 12 mg/mL


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In vivo
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