Methyl Vanillate

Catalog No.S3630 Batch:S363001

Technical Data

Formula	C₉H₁₀O₄
Molecular Weight	182.17	CAS No.	3943-74-6
Solubility (25°C)*	In vitro	DMSO	36 mg/mL (197.61 mM)
		Ethanol	36 mg/mL (197.61 mM)
		Water	12 mg/mL (65.87 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Methyl Vanillate, one of the ingredients in Hovenia dulcis Thunb, activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro.

Targets

Wnt/β-catenin ^[1]

In vitro

Methyl vanillate (MV) activates the Wnt/β-catenin pathway and induces osteoblast differentiation in vitro. It increases the expression of differentiation markers RUNX2, BMP2, ALP, and OCN in a dose-dependent manner. MV decreases and increases the expression of RANKL and OPG, respectively. Methyl vanillate dose-dependently elevates ALP activity in calvarial osteoblasts^[1].

In vivo

Methyl vanillate rescues trabecular or cortical femoral bone loss in the ovariectomized mice without inducing any significant weight changes or abnormality in liver tissue when administrated orally^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Calvarial osteoblasts Concentrations 10 and 20 μM Incubation Time 24 h Method Calvarial osteoblasts are seeded onto a coverslip in 12-well plates at a density of 3×10⁴ cells/well. After 24 h, HDT extract or MV is added for another 24 h and the cells are subsequently fixed with 4% PFA for 10 min. Cells are permeabilized with 0.2% Triton X-100 for 15 min and incubated for 30 min with 5% bovine serum albumin (BSA) blocking solution. The cells are incubated with the primary antibody (β-catenin) overnight at 4°C and then washed with PBS three times. Cells are then incubated with Alexa Fluor 502-conjugated IgG secondary antibody is incubated for 1 h, followed by incubation with DAPI for 5 min. The cells are mounted in Gel/Mount media. The fluorescence signal is captured using confocal microscopy.
Animal Study:^{^[1]}	Animal Models ICR mice Dosages 25 and 100 mg/kg Administration oral

Cell Assay:^{^[1]}

Cell lines
Calvarial osteoblasts
Concentrations
10 and 20 μM
Incubation Time
24 h
Method
Calvarial osteoblasts are seeded onto a coverslip in 12-well plates at a density of 3×10⁴ cells/well. After 24 h, HDT extract or MV is added for another 24 h and the cells are subsequently fixed with 4% PFA for 10 min. Cells are permeabilized with 0.2% Triton X-100 for 15 min and incubated for 30 min with 5% bovine serum albumin (BSA) blocking solution. The cells are incubated with the primary antibody (β-catenin) overnight at 4°C and then washed with PBS three times. Cells are then incubated with Alexa Fluor 502-conjugated IgG secondary antibody is incubated for 1 h, followed by incubation with DAPI for 5 min. The cells are mounted in Gel/Mount media. The fluorescence signal is captured using confocal microscopy.

Animal Study:^{^[1]}

Animal Models
ICR mice
Dosages
25 and 100 mg/kg
Administration
oral

References

[1] Cha PH, et al. PLoS One. 2014, 9(1):e85546.

Selleck's Methyl Vanillate has been cited by 1 publication

Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7]	PubMed: 34997030

Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7]

PubMed: 34997030

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.