Home Stem Cells & Wnt Wnt/beta-catenin activator - TGF-beta/Smad activator Isoxazole 9 (ISX-9) For research use only.

Isoxazole 9 (ISX-9)

Isoxazole 9 (Isx-9) is a synthetic promotor of adult neurogenesis by triggering neuronal differentiation of adult neural stem/precursor cells (NSPCs). Isoxazole 9 (Isx-9) activates multiple pathways including TGF-β induced epithelial–mesenchymal transition (EMT) signaling, canonical and non-canonical Wnt signaling at different stages of cardiac differentiation.

Isoxazole 9 (ISX-9) Chemical Structure

Isoxazole 9 (ISX-9) Chemical Structure

CAS No. 832115-62-5

Purity & Quality Control

Isoxazole 9 (ISX-9) Related Products

Signaling Pathway

Wnt/beta-catenin Signaling Pathways

Wnt/beta-catenin Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCN Function assay Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 5 uM Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of multiple of inhibitors 18552832
HCN Function assay 50 uM 3 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 25 uM 4 days Inhibition of gliogenesis differentiation in rat HCN cells at 25 uM after 4 days 18552832
HCN Function assay 5 uM Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis 18552832
HCN Function assay 5 uM Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 50 uM 3 hrs Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of multiple inhibitors 18552832
HCN Function assay Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells 18552832
HCN Function assay Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence multiple inhibitors 18552832
HCN Function assay 50 uM 24 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 50 uM 24 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine 18552832
HCN Function assay 50 uM 24 hrs Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of multiple inhibitors 18552832
HCN Function assay 8 to 64 uM 9 hrs Induction of neurogenesis in undifferentiated rat HCN cells assessed as MAP2AB protein level at 8 to 64 uM after 9 hrs by protein blotting analysis 18552832
HCN Function assay Induction of HDAC5 translocation in cytoplasm of rat HCN cells assessed as phosphorylated HDAC5 accumulation 18552832
HCN Function assay Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 2.5 uM potassium channel inhibitor KN92 18552832
HCN Function assay 8 to 64 uM 9 hrs Induction of neurogenesis in undifferentiated rat HCN cells assessed as GlR2/3 protein level at 8 to 64 uM after 9 hrs by protein blotting analysis 18552832
HCN Function assay 6 hrs Induction of HDAC5 phosphorylation in rat HCN cells after 6 hrs 18552832
HCN Function assay 24 hrs Induction of HDAC5 phosphorylation in rat HCN cells after 24 hrs 18552832
HCN Function assay Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as phosphorylated HDAC5 accumulation 18552832
HCN Function assay 5 uM Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 S258A S498A mutant fusion protein accumulation at 5 uM by fluorescence assay 18552832
HCN Function assay 50 uM 3 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine 18552832
HCN Function assay 20 uM Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 fusion protein accumulation at 20 uM by fluorescence assay 18552832
HCN Function assay Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 200 nM PKC inhibitor Go6976 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of multiple inhibitors 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist nifedipine 18552832
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Biological Activity

Description Isoxazole 9 (Isx-9) is a synthetic promotor of adult neurogenesis by triggering neuronal differentiation of adult neural stem/precursor cells (NSPCs). Isoxazole 9 (Isx-9) activates multiple pathways including TGF-β induced epithelial–mesenchymal transition (EMT) signaling, canonical and non-canonical Wnt signaling at different stages of cardiac differentiation.
In vitro
In vitro In NSPCs, isoxazole 9 increases cell number, and promotes cell differentiation. In OPCs, isoxazole 9 induces cell damage. In outgrowth EPCs, isoxazole 9 decreases tube formation without effect on early EPCs. [1]
Cell Research Cell lines NSPCs, OPCs, early EPCs, and outgrowth EPCs
Concentrations ~50 μM
Incubation Time 48 h
Method Cells are treated with different concentrations of Isx-9 (0, 6.25, 12.5, 25, or 50 μM), and 2 days later, cell numbers are counted in a blinded manner to evaluate the effects of Isx-9 on the proliferation/survival of four types of progenitor/precursor cells.
In Vivo
In vivo In mice, isoxazole 9 (20 mg/kg, i.p.) crosses the BBB and increases proliferation of neuroblasts and neurogenesis via Mef2-specific mechanisms in the hippocampal SGZ. Isoxazole 9 (20 mg/kg, i.p.) also increases differentiation and dendritic complexity of immature neurons and improves memory. In MWM, isoxazole 9 improves spatial memory. [2]
Animal Research Animal Models Transgenic Nkx2.5-luciferase reporter mice
Dosages 20 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 234.27 Formula

C11H10N2O2S
CAS No. 832115-62-5 SDF Download Isoxazole 9 (ISX-9) SDF
Smiles C1CC1NC(=O)C2=NOC(=C2)C3=CC=CS3
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 47 mg/mL ( (200.62 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 12 mg/mL

Water : Insoluble


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