Home Stem Cells & Wnt Wnt/beta-catenin inhibitor - PARP inhibitor JW55 For research use only.

JW55

JW55 is a potent and selective inhibitor of the canonical Wnt pathway that functions via inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).

JW55 Chemical Structure

JW55 Chemical Structure

CAS No. 664993-53-7

Purity & Quality Control

Batch: S674501 DMSO]87 mg/mL]false]Ethanol]4 mg/mL]false]Water]Insoluble]false Purity: 99.95%
99.95

JW55 Related Products

Signaling Pathway

Wnt/beta-catenin Signaling Pathways

Wnt/beta-catenin Signaling Pathway

Biological Activity

Description JW55 is a potent and selective inhibitor of the canonical Wnt pathway that functions via inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).
Targets
Wnt [1]
In vitro
In vitro

JW55 inhibits the PARP domain of TNKS1/2, leading to the stabilization of AXIN2 followed by increased degradation of β-catenin. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by JW55 with an IC50 value of 470 nmol/L. JW55 decreases auto-PARsylation of TNKS1/2 in vitro with IC50 values of 1.9 μmol/L and 830 nmol/L, respectively. JW55-mediated inhibition of canonical Wnt signaling results in reduced cell-cycle progression, proliferation, and colony formation in the CRC cell line SW480 in vitro[1].
Cell Research Cell lines SW480 cells
Concentrations 10, 5, or 1 μmol/L
Incubation Time 9 days
Method

--
In Vivo
In vivo

JW55 reduces XWnt8-induced axis duplication in Xenopus embryos and tamoxifen-induced polyposis formation in conditional APC mutant mice[1].
Animal Research Animal Models Apc(CKO/CKO)Lgr5-CreERT2+ mice
Dosages 100 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 434.49 Formula

C25H26N2O5
CAS No. 664993-53-7 SDF --
Smiles COC1=CC=C(C=C1)C2(CCOCC2)CNC(=O)C3=CC=C(C=C3)NC(=O)C4=CC=CO4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 87 mg/mL ( (200.23 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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