Home Stem Cells & Wnt Wnt/beta-catenin agonist WAY-262611 For research use only.

WAY-262611

Synonyms: BML-WN110

WAY-262611 (BML-WN110, Compound 5) is a beta-catenin (β-Catenin) agonist that increases bone formation rate with EC50 of 0.63 μM in TCF-Luciferase assay. WAY-262611 is also an inhibitor of dickkopf 1 (DKK1).

WAY-262611 Chemical Structure

WAY-262611 Chemical Structure

CAS No. 1123231-07-1

Purity & Quality Control

Batch: S982801 DMSO]64 mg/mL]false]Water]10 mg/mL]false]Ethanol]10 mg/mL]false Purity: 99.66%
99.66

WAY-262611 Related Products

Signaling Pathway

Wnt/beta-catenin Signaling Pathways

Wnt/beta-catenin Signaling Pathway

Biological Activity

Description WAY-262611 (BML-WN110, Compound 5) is a beta-catenin (β-Catenin) agonist that increases bone formation rate with EC50 of 0.63 μM in TCF-Luciferase assay. WAY-262611 is also an inhibitor of dickkopf 1 (DKK1).
Targets
DKK1 [2] β-Catenin [1]
(TCF-Luciferase assay)
0.63 μM(EC50)
In vitro
In vitro

WAY-262611, the DKK1 inhibitor, blocks DKK1 and restores the GDF5 reduced MMP13 gene expression.[2]
Cell Research Cell lines Surplus human chondrocytes
Concentrations 0.01 μM, 0.1 μM, 1 μM
Incubation Time 21 days
Method

The cell suspension is divided into aliquots of 200 μl to each well of an uncoated flat bottom 96-well plate. The plate is centrifuged at 1,500 rpm for 5 min and incubated for 48 h at 37°C in 5% CO2 and 90% relative humidity to allow for pellet formation. After 48 h the culture medium is changed to BDM supplemented with appropriate growth factors (GFs); 2, 10, 100 or 200 ng/ml recombinant GDF5, 50 ng/ml recombinant Wnt3a, 0.5 μM GSK3b inhibitor CHIR-99021, 0.01, 0.1 or 1 μM DKK1 inhibitor WAY-262611. Medium is changed three times per week for 21 days, whereafter the pellets are snap-frozen in liquid nitrogen and stored at -80°C.
In Vivo
In vivo

WAY-262611 has excellent pharmacokinetic properties and shows a dose dependent increase in the trabecular bone formation rate in ovariectomized rats following oral administration.[1]
Animal Research Animal Models OVX rats
Dosages 0.3 mg/kg, 1 mg/kg, 3 mg/kg, 10 mg/kg; 2 mg/kg
Administration Oral gavage; IV

Chemical Information & Solubility

Molecular Weight 318.42 Formula

C20H22N4
CAS No. 1123231-07-1 SDF --
Smiles NCC1CCN(CC1)C2=NC=CC(=N2)C3=CC=C4C=CC=CC4=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 64 mg/mL ( (200.99 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 10 mg/mL

Ethanol : 10 mg/mL


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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