Isoxazole 9 (ISX-9)

Catalog No.S7914 Batch:S791402

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Technical Data

Formula

C11H10N2O2S
Molecular Weight 234.27 CAS No. 832115-62-5
Solubility (25°C)* In vitro DMSO 46 mg/mL (196.35 mM)
Ethanol 28 mg/mL (119.52 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Isoxazole 9 (Isx-9) is a synthetic promotor of adult neurogenesis by triggering neuronal differentiation of adult neural stem/precursor cells (NSPCs). Isoxazole 9 (Isx-9) activates multiple pathways including TGF-β induced epithelial–mesenchymal transition (EMT) signaling, canonical and non-canonical Wnt signaling at different stages of cardiac differentiation.
In vitro In NSPCs, isoxazole 9 increases cell number, and promotes cell differentiation. In OPCs, isoxazole 9 induces cell damage. In outgrowth EPCs, isoxazole 9 decreases tube formation without effect on early EPCs. [1]
In vivo In mice, isoxazole 9 (20 mg/kg, i.p.) crosses the BBB and increases proliferation of neuroblasts and neurogenesis via Mef2-specific mechanisms in the hippocampal SGZ. Isoxazole 9 (20 mg/kg, i.p.) also increases differentiation and dendritic complexity of immature neurons and improves memory. In MWM, isoxazole 9 improves spatial memory. [2]

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    NSPCs, OPCs, early EPCs, and outgrowth EPCs

  • Concentrations

    ~50 μM

  • Incubation Time

    48 h

  • Method

    Cells are treated with different concentrations of Isx-9 (0, 6.25, 12.5, 25, or 50 μM), and 2 days later, cell numbers are counted in a blinded manner to evaluate the effects of Isx-9 on the proliferation/survival of four types of progenitor/precursor cells.
Animal Study:[2]
  • Animal Models

    Transgenic Nkx2.5-luciferase reporter mice

  • Dosages

    20 mg/kg

  • Administration

    i.p.

Selleck's Isoxazole 9 (ISX-9) has been cited by 3 publications

ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude [ Commun Biol, 2022, 5(1):750] PubMed: 35902736
Cochlear Sox2+ Glial Cells Are Potent Progenitors for Spiral Ganglion Neuron Reprogramming Induced by Small Molecules [ Front Cell Dev Biol, 2021, 9:728352] PubMed: 34621745
Cochlear Sox2+ Glial Cells Are Potent Progenitors for Spiral Ganglion Neuron Reprogramming Induced by Small Molecules [ Front Cell Dev Biol, 2021, 9:728352] PubMed: 34621745

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.