Home GPCR & G Protein Dyes inhibitor - Guanylate Cyclase inhibitor - Microtubule Associated inhibitor Methylene Blue For research use only.

Methylene Blue

Synonyms: Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015

Methylene Blue (Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015) is used as a dye in chromoendoscopy. It Inhibits tau filament formation with IC50 of 1.9μM. Also it inhibits soluble guanylyl cyclase.

Methylene Blue Chemical Structure

Methylene Blue Chemical Structure

CAS No. 61-73-4

Purity & Quality Control

Methylene Blue Related Products

Biological Activity

Description Methylene Blue (Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015) is used as a dye in chromoendoscopy. It Inhibits tau filament formation with IC50 of 1.9μM. Also it inhibits soluble guanylyl cyclase.
Targets
guanylate cyclase [3] Tau aggregation [2]
1.9 μM
In vitro
In vitro MB may inhibit the action of nitric oxide synthase itself and could also inactivate nitric oxide directly, possibly through the generation of superoxide anions. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, MB has also been shown to directly activate calcium-dependent potassium channels and to enhance release of noradrenaline from intracellular stores[1].
Cell Research Cell lines Immature neuroblasts
Concentrations 10 μM
Incubation Time 2 h
Method

single cell culture model where immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are usd for investigations. Cultures are exposed at the sixth day in vitro to MB for 2 h and then we assess the survival and dendritic arbor architecture of these cells 48 h after this treatment paradigm.
In Vivo
In vivo Methylene Blue acts by inhibiting guanylate cyclase, thus decreasing C-GMP and vascular smooth muscle relaxation. When injected intravenously, MB selectively accumulates in parathyroid glands, thereby facilitating the identification of these structures during surgery. Pharmacokinetic studies in rodents revealed that intravascular administration of MB leads to a rapid and extensive accumulation of this drug in the central nervous system (CNS)[1].
Animal Research Animal Models Sprague- Dawley male rats
Dosages 5 and 50 mg/kg
Administration intravascular injection

Chemical Information & Solubility

Molecular Weight 319.85 Formula

C16H18ClN3S
CAS No. 61-73-4 SDF Download Methylene Blue SDF
Smiles CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.[Cl-]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 63 mg/mL ( (196.96 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 63 mg/mL

Ethanol : 10 mg/mL


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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