Home Endocrinology & Hormones Estrogen/progestogen Receptor agonist Medroxyprogesterone For research use only.

Medroxyprogesterone

Synonyms: MP

Medroxyprogesterone (MP) is a synthetic pregnane steroid and a derivative of progesterone. It is a potent progesterone receptor agonist.

Medroxyprogesterone Chemical Structure

Medroxyprogesterone Chemical Structure

CAS No. 520-85-4

Purity & Quality Control

Medroxyprogesterone Related Products

Signaling Pathway

Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway

Biological Activity

Description Medroxyprogesterone (MP) is a synthetic pregnane steroid and a derivative of progesterone. It is a potent progesterone receptor agonist.
In vitro
In vitro Medroxyprogesterone acetate (MPA, 10 nM) treatment for 48 h induces proliferation of the cells (1.6-fold induction). MPA induces cyclin D1 expression (3.3-fold induction). MPA increases both the protein level (2.2-fold induction) and promoter activity (2.7-fold induction) of cyclin D1 in MCF-7 cells transfected with PRB but not with PRA. Although MPA transcriptionally activates cyclin D1 expression, cyclin D1 promoter does not have progesterone-responsive element-related sequence. MPA induces the transient phosphorylation of Akt (2.7-fold induction at 5 min) and also phosphorylation of inhibitor of NFkappaBalpha (IkappaBalpha) (2.3-fold induction)[1]. MPA activates glucocorticoid receptors, which could mimic part of the anti-atherosclerotic effects of glucocorticoids, and has, on the other hand, also anti-androgenic activity, which might diminish protective oestrogen effects[2].
Cell Research Cell lines T47D human breast cancer cells
Concentrations 10 nM
Incubation Time 4 days
Method The cells are plated at a density of 10 or 40 × 104 cells/well in 12-well plates and allowed to attach overnight. The cells are then growth arrested by incubation in phenol red-free DMEM with 10% charcoal-stripped serum for 24 h followed by treatment with vehicle or MPA by exchanging the culture medium containing these agents with fresh medium every 24 h for 4 d. A Neubauer chamber is used to count cells, and trypan blue experiments are carried out in quadruplicate.
In Vivo
In vivo Long-term treatment with MPA and MPA + E2 increases arterial thrombosis despite inhibitory effects of MPA on atherosclerosis in ApoE-deficient mice. Increased thrombin formation, reduced smooth muscle content and remodelling of non-collagenous plaque matrix may be involved in the pro-thrombotic effects. Thus, MPA exhibits differential effects on arterial thrombosis and on atherosclerosis. In monkeys, MPA interferes with anti-atherosclerotic oestrogen effects whereas progesterone does not[2].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05188222 Not yet recruiting
Left Ventricular Dysfunction|Quality of Recovery|Right Ventricular Dysfunction
Jewish General Hospital
 September 1 2024 Phase 4
NCT06325293 Not yet recruiting
Community Acquired Pneumonia in Children|Mycoplasma Pneumoniae|Mycoplasma Pneumoniae Pneumonia
Christoph Berger|University Children''s Hospital Zurich
 July 1 2024 Not Applicable

Chemical Information & Solubility

Molecular Weight 344.49 Formula

C22H32O3
CAS No. 520-85-4 SDF Download Medroxyprogesterone SDF
Smiles CC1CC2C(CCC3(C2CCC3(C(=O)C)O)C)C4(C1=CC(=O)CC4)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 68 mg/mL ( (197.39 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 14 mg/mL

Water : Insoluble


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In vivo
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