Home Proteases Serine Protease inhibitor - Cysteine Protease inhibitor - Cathepsin B inhibitor Leupeptin Hemisulfate For research use only.

Leupeptin Hemisulfate

Leupeptin Hemisulfate is a reversible inhibitor of serine and cysteine proteases. It inhibits cathepsin B (Ki = 6 nM), calpain (Ki = 10 nM), trypsin (Ki = 35 nM), plasmin (Ki = 3.4 μM), and kallikrein (Ki = 19 μM), and has no effect against chymotrypsin, elastase, renin, or pepsin.

Leupeptin Hemisulfate Chemical Structure

Leupeptin Hemisulfate Chemical Structure

CAS No. 103476-89-7

Purity & Quality Control

Leupeptin Hemisulfate Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 Function assay 20 mins Inhibition of human recombinant matriptase catalytic domain expressed in HEK293 cells after 20 mins, Ki = 1.9 μM. 20684597
HEK293 Function assay 20 mins Inhibition of human recombinant matriptase 2 expressed in HEK293 cells in conditioned medium after 20 mins, Ki = 2.4 μM. 20684597
HEK293 Function assay 20 mins Inhibition of human purified matriptase 2 catalytic domain expressed in HEK293 cells after 20 mins, Ki = 4.1 μM. 20684597
Click to View More Cell Line Experimental Data

Biological Activity

Description Leupeptin Hemisulfate is a reversible inhibitor of serine and cysteine proteases. It inhibits cathepsin B (Ki = 6 nM), calpain (Ki = 10 nM), trypsin (Ki = 35 nM), plasmin (Ki = 3.4 μM), and kallikrein (Ki = 19 μM), and has no effect against chymotrypsin, elastase, renin, or pepsin.
Targets
Cysteine protease [1] serine protease [1]
In vitro
In vitro

Leupeptin, produced by various species of actinomycetes, strongly inhibit proteolysis. [1]

Leupeptin hemisulfate protects tubulin from endogenous proteolytic activities during the isolation procedure and results in increased tubulin purity. [2]

Leupeptin hemisulfate could restore up to 50% of hepatitis B surface antigen (HBsAg) expression in cell suspension cultures. [3]

Chemical Information & Solubility

Molecular Weight 475.59 Formula

C20H38N6O4.1/2H2SO4
CAS No. 103476-89-7 SDF Download Leupeptin Hemisulfate SDF
Smiles CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCCN=C(N)N)C=O)NC(=O)C.CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCCN=C(N)N)C=O)NC(=O)C.OS(=O)(=O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 95 mg/mL ( (199.75 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 95 mg/mL

Ethanol : 95 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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