Leupeptin Hemisulfate

Catalog No.S7380 Batch:S738018

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Technical Data

Formula

C20H38N6O4.1/2H2SO4
Molecular Weight 475.59 CAS No. 103476-89-7
Solubility (25°C)* In vitro DMSO 95 mg/mL (199.75 mM)
Water 95 mg/mL (199.75 mM)
Ethanol 95 mg/mL (199.75 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Leupeptin Hemisulfate is a reversible inhibitor of serine and cysteine proteases. It inhibits cathepsin B (Ki = 6 nM), calpain (Ki = 10 nM), trypsin (Ki = 35 nM), plasmin (Ki = 3.4 μM), and kallikrein (Ki = 19 μM), and has no effect against chymotrypsin, elastase, renin, or pepsin.
Targets
Cysteine protease [1] serine protease [1]
In vitro

Leupeptin, produced by various species of actinomycetes, strongly inhibit proteolysis. [1]

Leupeptin hemisulfate protects tubulin from endogenous proteolytic activities during the isolation procedure and results in increased tubulin purity. [2]

Leupeptin hemisulfate could restore up to 50% of hepatitis B surface antigen (HBsAg) expression in cell suspension cultures. [3]

Protocol (from reference)

Animal Study:

[4]
  • Animal Models

    C57BL/6NCrl male mice

  • Dosages

    20 mg/kg

  • Administration

    i.p.

Selleck's Leupeptin Hemisulfate has been cited by 34 publications

FOXS1, frequently inactivated by promoter methylation, inhibited colorectal cancer cell growth by promoting TGFBI degradation through autophagy-lysosome pathway [ J Adv Res, 2025, S2090-1232(25)00056-6] PubMed: 39864590
Targeting deubiquitinase USP7-mediated stabilization of XPO1 contributes to the anti-myeloma effects of selinexor [ J Transl Med, 2025, 23(1):62] PubMed: 39806439
Hepatic Lamp2a deficiency promotes inflammation of murine autoimmune cholangitis via affecting bile acid metabolism [ iScience, 2025, 28(2):111804] PubMed: 39995863
Bafilomycin A1 induces colon cancer cell death through impairment of the endolysosome system dependent on iron [ Sci Rep, 2025, 15(1):5148] PubMed: 39934167
ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade [ Nat Commun, 2023, 14(1):4066] PubMed: 37429863
Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway [ Acta Pharm Sin B, 2022, 12(6):2869-2886] PubMed: 35755273
18F-FDG PET as an imaging biomarker for the response to FGFR-targeted therapy of cancer cells via FGFR-initiated mTOR/HK2 axis [ Theranostics, 2022, 12(14):6395-6408] PubMed: 36168616
CLP36 promotes p53 deficient sarcoma progression through suppression of atrophin-1 interacting protein-4 (AIP-4)-dependent degradation of YAP1 [ Theranostics, 2022, 12(11):5051-5068] PubMed: 35836803
Histone deacetylase 3 contributes to the antiviral innate immunity of macrophages by interacting with FOXK1 to regulate STAT1/2 transcription [ Cell Rep, 2022, 38(4):110302] PubMed: 35081346
Histone deacetylase 3 facilitates TNFα-mediated NF-κB activation through suppressing CTSB induced RIP1 degradation and is required for host defense against bacterial infection [ Cell Biosci, 2022, 12(1):81] PubMed: 35658939

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.