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Leonurine

Leonurine, an active alkaloid extracted from Traditional Chinese Medicine Herba leonuri, exerts several biological effects, such as antidiabetic, cardiovascular, and bovine mastitis protection.

Leonurine Chemical Structure

Leonurine Chemical Structure

CAS No. 24697-74-3

Purity & Quality Control

Batch: S389001 DMSO]Insoluble]false]]]false]]]false Purity: 99.74%
99.74

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Biological Activity

Description Leonurine, an active alkaloid extracted from Traditional Chinese Medicine Herba leonuri, exerts several biological effects, such as antidiabetic, cardiovascular, and bovine mastitis protection.
In vitro
In vitro Leonurine inhibits U937 cells adhesion to TNF-α-activated HUVEC in a concentration dependent manner. Treatment with leonurine blocks TNF-α-induced mRNA and protein expression of adhesion molecules (intercellular adhesion molecule-1 and vascular cell adhesion molecule-1), cyclooxygenase-2, and monocyte chemoattractant protein-1 in endothelial cells. In addition, leonurine attenuates TNF-α-induced intracellular ROS production in HUVEC. Furthermore, leonurine suppresses the TNF-α-activated p38 phosphorylation and IκBα degradation. Subsequently reduced NF-κB p65 phosphorylation, nuclear translocation, and DNA-binding activity are also observed[1]. Leonurine attenuates DOX-induced apoptosis in H9c2 cell by increasing anti-oxidant, anti-apoptotic ability and protecting mitochondrial function[4].
Cell Research Cell lines Rat embryonic ventricularmyocardial H9c2 cell line
Concentrations 10 μM
Incubation Time 2 h
Method

Cells are using for Hoechst staining after treatement. DOX treatment: cells are exposed to 1 μM DOX for 24 h; Leo treatment: cells are pre-treated with 10 μM Leo for 2 h before exposure to DOX.
In Vivo
In vivo Leonurine attenuates hyperalgesia in mice with induced adenomyosis via down-regulating expressions of p-P65, COX-2, and OTR, and could be beneficial for treating adenomyosis[2]. Leonurine exerts antidepressant-like effects, which may be mediated, at least in part, by improving monoamine neurotransmitters and inhibiting neuroinflammation. Leonurine possesses neuroprotective effects in animal models of ischemic stroke, parkinson's disease, and alzheimer's disease. Leonurine treatment significantly rescues behavioral deficit of animals, promotes neuronal survival, and inhibits inflammation[3].
Animal Research Animal Models Male C57BL/6 (8–10 weeks) mice
Dosages 30 and 60 mg/kg
Administration intragastric administration

Chemical Information & Solubility

Molecular Weight 311.33 Formula

C14H21N3O5
CAS No. 24697-74-3 SDF Download Leonurine SDF
Smiles COC1=CC(=CC(=C1O)OC)C(=O)OCCCCN=C(N)N
Storage (From the date of receipt)

In vitro
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DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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