Leonurine

Catalog No.S3890 Batch:S389001

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Technical Data

Formula

C14H21N3O5
Molecular Weight 311.33 CAS No. 24697-74-3
Solubility (25°C)* In vitro DMSO Insoluble
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Leonurine, an active alkaloid extracted from Traditional Chinese Medicine Herba leonuri, exerts several biological effects, such as antidiabetic, cardiovascular, and bovine mastitis protection.
In vitro Leonurine inhibits U937 cells adhesion to TNF-α-activated HUVEC in a concentration dependent manner. Treatment with leonurine blocks TNF-α-induced mRNA and protein expression of adhesion molecules (intercellular adhesion molecule-1 and vascular cell adhesion molecule-1), cyclooxygenase-2, and monocyte chemoattractant protein-1 in endothelial cells. In addition, leonurine attenuates TNF-α-induced intracellular ROS production in HUVEC. Furthermore, leonurine suppresses the TNF-α-activated p38 phosphorylation and IκBα degradation. Subsequently reduced NF-κB p65 phosphorylation, nuclear translocation, and DNA-binding activity are also observed[1]. Leonurine attenuates DOX-induced apoptosis in H9c2 cell by increasing anti-oxidant, anti-apoptotic ability and protecting mitochondrial function[4].
In vivo Leonurine attenuates hyperalgesia in mice with induced adenomyosis via down-regulating expressions of p-P65, COX-2, and OTR, and could be beneficial for treating adenomyosis[2]. Leonurine exerts antidepressant-like effects, which may be mediated, at least in part, by improving monoamine neurotransmitters and inhibiting neuroinflammation. Leonurine possesses neuroprotective effects in animal models of ischemic stroke, parkinson's disease, and alzheimer's disease. Leonurine treatment significantly rescues behavioral deficit of animals, promotes neuronal survival, and inhibits inflammation[3].

Protocol (from reference)

Cell Assay:

[4]
  • Cell lines

    Rat embryonic ventricularmyocardial H9c2 cell line

  • Concentrations

    10 μM

  • Incubation Time

    2 h

  • Method

    Cells are using for Hoechst staining after treatement. DOX treatment: cells are exposed to 1 μM DOX for 24 h; Leo treatment: cells are pre-treated with 10 μM Leo for 2 h before exposure to DOX.
Animal Study:

[3]
  • Animal Models

    Male C57BL/6 (8–10 weeks) mice

  • Dosages

    30 and 60 mg/kg

  • Administration

    intragastric administration

Selleck's Leonurine has been cited by 1 publication

Novel insights into the protective effects of leonurine against acute kidney injury: Inhibition of ER stress-associated ferroptosis via regulating ATF4/CHOP/ACSL4 pathway [ Chem Biol Interact, 2024, 395:111016] PubMed: 38670420

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.