Iodonitrotetrazolium chloride

Synonyms: INT

Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator, hence INT can be used for the colorimetric assay of various dehydrogenases.

Iodonitrotetrazolium chloride Chemical Structure

Iodonitrotetrazolium chloride Chemical Structure

CAS No. 146-68-9

Purity & Quality Control

Batch: E268701 DMSO]100 mg/mL]false]Ethanol]13 mg/mL]false]Water]Insoluble]false Purity: 98.35%
98.35

Iodonitrotetrazolium chloride Related Products

Biological Activity

Description Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator, hence INT can be used for the colorimetric assay of various dehydrogenases.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06358573 Not yet recruiting
Triple-negative Breast Cancer|TNBC - Triple-Negative Breast Cancer
Swiss Group for Clinical Cancer Research
June 30 2024 Phase 2
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Post Graduate Institute of Medical Education and Research Chandigarh
April 24 2024 Phase 2|Phase 3

Chemical Information & Solubility

Molecular Weight 505.7 Formula

C19H13IN5O2.Cl

CAS No. 146-68-9 SDF --
Smiles [Cl-].[O-][N+](=O)C1=CC=C(C=C1)[N+]2=NC(=N[N]2C3=CC=C(I)C=C3)C4=CC=CC=C4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (197.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 13 mg/mL

Water : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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