Home Apoptosis Wnt/beta-catenin activator - Apoptosis related inhibitor HLY78 For research use only.

HLY78

HLY78 inhibits apoptosis in tumor cells and embryonic cells caused by carbon ion radiation through activation of the Wnt/β-catenin pathway.

HLY78 Chemical Structure

HLY78 Chemical Structure

CAS No. 854847-61-3

Purity & Quality Control

Batch: S727401 DMSO]53 mg/mL]false]Ethanol]26 mg/mL]false]Water]Insoluble]false Purity: 99.83%
99.83

HLY78 Related Products

Signaling Pathway

Wnt/beta-catenin Signaling Pathways

Wnt/beta-catenin Signaling Pathway

Biological Activity

Description HLY78 inhibits apoptosis in tumor cells and embryonic cells caused by carbon ion radiation through activation of the Wnt/β-catenin pathway.
In vitro
In vitro

HLY78 is an activator for the Wnt/β-catenin signaling pathway, which acts upstream of β-catenin and functions only in the presence of the Wnt3a ligand, and targets the DIX domain of Axin and potentiates the Axin-LRP6 association, thus promoting LRP6 phosphorylation and Wnt signaling transduction.[1]
Cell Research Cell lines HEK293T cells
Concentrations 2.5, 5, 10, 20 μM
Incubation Time 1+6 h
Method

The HEK293T cells were transfected with the topFlash plasmid, Δn-β-catenin or both. the cells were treated 18 h later for 1 h with DMSO or HLY78 followed by the control conditioned medium, the Wnt3a conditioned medium or 8 mM LiCl plus the same dose of HLY78 for an additional 6 h before the luciferase activity assays.
In Vivo
In vivo

HLY78 attenuates neuronal apoptosis and the neurological deficits after SAH through the activation of low-density lipoprotein receptor-related protein 6 (LRP6), which subsequently increased the level of phosphorylated glycogen synthesis kinase 3 beta (p-GSK3β) (Ser9), β-catenin, and Bcl-2, accompanied by a decrease of p-β-catenin, Bax, and cleaved caspase 3.[2]

Chemical Information & Solubility

Molecular Weight 267.32 Formula

C17H17NO2
CAS No. 854847-61-3 SDF --
Smiles CCC1=C2N(C)CC3=CC4=C(OCO4)C=C3C2=CC=C1
Storage (From the date of receipt) 3 years-21°C powder

In vitro
Batch:

DMSO : 53 mg/mL ( (198.26 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 26 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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