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Fer-1 (Ferrostatin-1)

Name: Fer-1 (Ferrostatin-1)
Brand: Selleck Chemicals
SKU: S7243
Rating: 5 (482 reviews)

Catalog No.S7243 Purity:99.98%

Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.

Fer-1 (Ferrostatin-1) Chemical Structure

CAS No. 347174-05-4

Purity & Quality Control

Batch: Purity: 99.98%

99.98

Products often used together with Fer-1 (Ferrostatin-1)

RSL3

Ferrostatin-1 and RSL3 combination significantly inhibit NCX4040 cytotoxicity in colorectal cancer (CRC) cells, HT-29/HCT 116.

Sinha BK, et al. Cells. 2023 Jun 14;12(12):1626.

Z-VAD-FMK

Ferrostatin-1 and Z-VAD-FMK combination block the cell viability inhibited by CAY10566 in ovarian cancer cell lines OVCAR-5.

Zhao G, et al. Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2203480119.

Fer-1 (Ferrostatin-1) Related Products

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Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
human IMR32 neuroblastoma cells	Function assay		13 h	Antiferroptotic activity in human IMR32 neuroblastoma cells assessed as inhibition of erastin-induced cell death preincubated for 1 hr before erastin stimulation and measured after 13 hrs by fluorescence plate reader method, IC50=0.018 μM	26696014
HT1080 cells	Function assay			Antiferroptotic activity in human HT1080 cells assessed as inhibition of erastin-induced cell death by Alamar blue assay, IC50=0.095 μM	26696014
HRE cells	Cell viability assay	1 μM	48-72 hours	Induction of cell apoptosis	30794682
U57810 and C2C12 cells	Cell viability assay	10 μM	24, 48, and 72 h	Administration of ferrostatin-1 almost completely prevented the ferroptotic cell death.	29971532
Leishmania major Friedlin clone V1	Antileishmanial assay		72 h	LD50 = 28.75 μM	26410073
IMR32	Function assay		1 h	IC50 = 0.033 μM	30354101
Click to View More Cell Line Experimental Data

Biological Activity

Description

Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.

Targets

Ferroptosis ^[1]
(HT-1080 cells)

60 nM(EC50)

In vitro
In vitro	Ferrostatin-1 (2 μM) prevents erastin-induced ferroptosis in cancer cells, as well as glutamate-induced cell death in postnatal rat brain slices. Ferrostatin-1 is a lipid ROS scavenger, with the N-cyclohexyl moiety serving as a lipo-philic anchor within biological membranes. Ferrostatin-1 does not inhibit extracellular signal -regulated kinase (ERK) phos-phorylation or arrest the proliferation of HT-1080 cells, suggesting that it does not inhibit the MEK/ERK pathway, chelate iron, or inhibit protein synthesis. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 readily oxidizes the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) under cell-free conditions. ^[1]
Cell Research	Cell lines	HT-1080 cells
	Concentrations	60 nM (EC50)
	Incubation Time	24 h
	Method	Erastin treated cells were incubated with ferrostatin-1 at 37˚C for 24 hr and viability was assessed using Alamar Blue assay.
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	Nrf2 / Gpx4 / β-Actin gp91 phox / β-actin KIM-1 / NGAL / β-actin Collagen I / β-actin cleaved caspase-3 / β-actin		31571665
	Growth inhibition assay	Cell viability Cell viability		31899616
	Immunofluorescence	γH2AX morphological features of HT-22 cells renal apoptosis ROS formation		31899616

In Vivo
In vivo	Ferrostatin-1 (Fer-1), a synthetic antioxidant, is a potent and selective inhibitor of ferroptosis. It acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death.^[2]
Animal Research	Animal Models	C57BL/6 mice
	Dosages	10 mg/kg
	Administration	i.n.

References

Chemical Information & Solubility

Molecular Weight	262.35	Formula	C₁₅H₂₂N₂O₂
CAS No.	347174-05-4	SDF	Download Fer-1 (Ferrostatin-1) SDF
Smiles	CCOC(=O)C1=CC(=C(C=C1)NC2CCCCC2)N
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 55 mg/mL ( (209.64 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 55 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	5% DMSO 1 40% PEG 300 2 5%tween 80 3 50%ddH2O	1.0mg/ml (3.81mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O Dilute to 1 mL. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	2% DMSO 1 50% PEG 300 2 5% Tween 80 3 ddH2O	1.0mg/ml (3.81mM)	Taking the 1 mL working solution as an example, add 20 μL of 50 mg/ml clarified DMSO stock solution to 500 μL PEG300, mix evenly to clarify it; add 50 μL Tween-80 to the above system, mix evenly to clarify it; Then continue to add 430 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	1.65mg/ml (6.29mM)	Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml (19.06mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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