Home Metabolism Ferroptosis inhibitor Liproxstatin-1 For research use only.

Liproxstatin-1

Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.

Liproxstatin-1 Chemical Structure

Liproxstatin-1 Chemical Structure

CAS No. 950455-15-9

Purity & Quality Control

Products often used together with Liproxstatin-1

RSL3

Liproxstatin-1 and RSL3, in combination with iFSP1, induce cell death in human esophageal squamous cell carcinoma (ESCC) cell lines KYSE30/KYSE510/KYSE520.

Miyauchi W, et al. Esophagus. 2023 Jul;20(3):492-501.

DHA (Dihydroartemisinin)

Liproxstatin-1 and Dihydroartemisinin (DHA) have an antagonistic effect on the growth of blood-stage parasites of malaria.

Li S, et al. Biomed Pharmacother. 2022 Apr;148:112742.

iFSP1

Liproxstatin-1 and RSL3 combination induce cell death in human esophageal squamous cell carcinoma (ESCC) cell lines KYSE30/KYSE510/KYSE520.

Miyauchi W, et al. Esophagus. 2023 Jul;20(3):492-501.

S63845

Liproxstatin-1 completely protects BAX/BAK double knock-out (DKO) U251 cells from cell death induced by the combination of S63845 and A1331852.

Moujalled D, et al. Cell Death Differ. 2022 Jul;29(7):1335-1348.

A-1331852

Liproxstatin-1 effectively reduces cell death of U251 cells when treated with A1331852 plus S63845.

Moujalled D, et al. Cell Death Differ. 2022 Jul;29(7):1335-1348.

Liproxstatin-1 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC-5 lung fibroblast cells DNDI: Cytotoxicity against human MRC-5 lung fibroblast cells, IC50 = 9.14 μM ChEMBL
Click to View More Cell Line Experimental Data

Biological Activity

Description Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.
Targets
ferroptosis [1]
(Cell-free assay)
22 nM
In vitro
In vitro Liproxstatin-1 is able to inhibit ferroptosis in the low nanomolar range and inhibit the growth of Gpx4−/−cells with IC50 of 22 nM. Liproxstatin-1 (50 nM) completely prevents lipid peroxidation in Gpx4−/−cells. Liproxstatin-1 (200 nM) protects against FINs, such as BSO (10 µM), erastin (1 µM) and RSL3 (0.5 µM), in a dose dependent manner, whereas it fails to rescue cell death induced by staurosporine (0.2 µM) and H2O2 (200 µM).[1]
Cell Research Cell lines Gpx4−/−cells
Concentrations ~200 nM
Incubation Time ~72 h
Method

Cell viability is assessed at different time points after treatment using AquaBluer according to the manufacturer’s recommendations. Alternatively, cell death is also quantified by measuring released lactate dehydrogenase activity using the cytotoxicity detection kit (LDH).
Experimental Result Images Methods Biomarkers Images PMID
Western blot COX2 / GPx4 / GAPDH β-actin / COX2 / GPx4 β-actin / COX2 / GPx4 NeuN / Syn / SNAP / GFAP / Actin / Tubulin GRP78 / CHOP / Actin GRP78 / CHOP / Actin GPX4 / β-actin 33889074
Growth inhibition assay Cell viability 31641008
Immunofluorescence β-actin / COX2 / GPx4 NeuN / ACSL4 mitochondrial Fe2+ 30737476
In Vivo
In vivo Liproxstatin-1 remarkably extends survival compared with the vehicle-treated group, delays ferroptosis in tubular cells, and mitigates tissue injury in ischaemia/reperfusion-induced liver injury. [1]
Animal Research Animal Models GreERT2; Gpx4fI/fI mice
Dosages 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 340.85 Formula

C19H21ClN4
CAS No. 950455-15-9 SDF Download Liproxstatin-1 SDF
Smiles C1CNCCC12C(=NCC3=CC(=CC=C3)Cl)NC4=CC=CC=C4N2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 68 mg/mL ( (199.5 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 18 mg/mL

Water : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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